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Stevia: A Natural Non-nutritive Sweetener and its Potential
                                Application in Food Products
                                                 Som Nath Khanal1 and Yakindra Prasad Timilsena2*
1SouthDakota State University, Brookings, SD, 57007, USA.
2Department of Food Technology and Quality Control, Babarmahal, Kathmandu, Nepal

Email: yakendra.timilsena@gmail.com

                                      Abstract                                          Composition and Processing Aspects
        Stevia rebaudiana is a small, perennial, semi-humid, and
        subtropical plant containing several sweetening compounds.
        Originated from Paraguay, Stevia has been used since ancient
        times for various purposes including as a sweetener. As the
        leaves of Stevia plants contain Stevioside, the sweet
        component 150 to 300 times sweeter than sucrose, but with
        no calorific value, they have functional and sensory properties
        superior to those of many other high-potency sweeteners.                                                                   Fatty acid composition
        This is the reason, Stevia has been considered as a major
        source of high-potency sweetener for the growing natural                                                                Fatty acids                contents
                                                                                                                                                           (g/100g)
        food market. In this presentation, different scientific and                                                             Palmitic acid (C16)        27.51
        technological aspects of Stevia plant and Stevia derived                                                                Palmitoleic acid (C16-1)   1.27
        sweeteners are described. This presentation tends to touch                                                              Stearic acid (C18)         1.18
        Stevia                                              agriculture;                                                        Oleic acid (C18-1)         4.36
        however, extraction, purification, processing, and application                                                          Linoleic acid (C18-2)      12.40
        of the stevia derived sweeteners are given major focus.                                                                 Linolenic acid (C18-3)     21.59
                                                                                    (Savita et al., 2004)
                                                                                                                                (Tadhani and Subhash, 2006)
                                  Objective
                                                                                   Sweet components
 • Explore the agricultural and processing technologies
 • Describe the chemical and biological properties of                              • Two main glycosides, Stevioside (St) and Rebaudioside A (R-A)
   stevia components and their food safety concerns                                  are sweet
                                                                                   • Stevioside makes up the majority of the sweetener (60 – 70% of
 • Highlight its potential application as non-nutritive
                                                                                     the total), 110 – 270 times sweeter than sugar, licorice like slight
   natural sweetener                                                                 after taste.
                                                                                   • Rebaudioside A is usually present as 30 – 40% of total
                               Introduction                                          sweetener,;180 – 400 times sweeter than sugar with no after-
                                                                                     taste.
        Origin                                                                     • The ratio R-A/St is the measure of sweetness quality – the more
                                                                                     R-A the better.
    •     Dr M.S. Bertoni discovered                                               • The minor glycosides are considered to be less sweet, 30 – 80
          Stevia rebaudiana in Paraguay in 1888.                                     times sweeter than sugar
    •     The plant was named in 1905 in honour
          of Paraguayan chemist Dr. Rebaudi.
    •     150 Stevia species have been identified but Stevia rebaudiana
          is the sweetest of all.                                                  Processing

    The Stevia Farming
    The native habitat-
           • latitude of 25° S in a subtropical
             region of northeastern Paraguay                                       Two sweet products of stevia exist
           • between 500–1,500 m above sea level,                                     • dried stevia leaf or powder
           • soil having a low phosphate content                                      • Purified stevia glycoside powder (commercialized)
           • annual average temperature of 75°F,
           • average rainfall of about 55 in. per year                             Processing involves four basic steps:
    • Seedlings are transplanted in April or May (on the basis of                  • Dissolving the sweetener in boiling water or other solvent
       study in Japan)                                                                (Methanol)
    • Requires frequent irrigation                                                 • Ion-exchange, membrane technology for purification and
    • is a poor competitor with weeds,                                                concentration
    • warm temperatures with minimal frost, and high light                         • Filtration with precipitation/coagulation
       intensities are required.                                                   • Crystallisation and drying.
    • Acidic soil pH (pH 4–5) favors rapid growth (80 cm).
    • Can be harvested in Summer and Autumn.                                                                  Conclusion
                                                                                   • Non-nutritive natural sweetener with high sweetness
                      Use of Stevia products                                         and medicinal value
•        Japan is the biggest user.                                                • No food safety concern, appropriate for diabetes
•        Approved by USFDA as a food additive                                      • Developed processing techniques
•        Can be used in most of the foods.
•        Preferably used in drinks
                                                                                   • Potential high value cash crop for Nepalese agri-sector.

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Stevia a natural non-nutritive sweetener and its potential application in food products

  • 1. Stevia: A Natural Non-nutritive Sweetener and its Potential Application in Food Products Som Nath Khanal1 and Yakindra Prasad Timilsena2* 1SouthDakota State University, Brookings, SD, 57007, USA. 2Department of Food Technology and Quality Control, Babarmahal, Kathmandu, Nepal Email: yakendra.timilsena@gmail.com Abstract Composition and Processing Aspects Stevia rebaudiana is a small, perennial, semi-humid, and subtropical plant containing several sweetening compounds. Originated from Paraguay, Stevia has been used since ancient times for various purposes including as a sweetener. As the leaves of Stevia plants contain Stevioside, the sweet component 150 to 300 times sweeter than sucrose, but with no calorific value, they have functional and sensory properties superior to those of many other high-potency sweeteners. Fatty acid composition This is the reason, Stevia has been considered as a major source of high-potency sweetener for the growing natural Fatty acids contents (g/100g) food market. In this presentation, different scientific and Palmitic acid (C16) 27.51 technological aspects of Stevia plant and Stevia derived Palmitoleic acid (C16-1) 1.27 sweeteners are described. This presentation tends to touch Stearic acid (C18) 1.18 Stevia agriculture; Oleic acid (C18-1) 4.36 however, extraction, purification, processing, and application Linoleic acid (C18-2) 12.40 of the stevia derived sweeteners are given major focus. Linolenic acid (C18-3) 21.59 (Savita et al., 2004) (Tadhani and Subhash, 2006) Objective Sweet components • Explore the agricultural and processing technologies • Describe the chemical and biological properties of • Two main glycosides, Stevioside (St) and Rebaudioside A (R-A) stevia components and their food safety concerns are sweet • Stevioside makes up the majority of the sweetener (60 – 70% of • Highlight its potential application as non-nutritive the total), 110 – 270 times sweeter than sugar, licorice like slight natural sweetener after taste. • Rebaudioside A is usually present as 30 – 40% of total Introduction sweetener,;180 – 400 times sweeter than sugar with no after- taste. Origin • The ratio R-A/St is the measure of sweetness quality – the more R-A the better. • Dr M.S. Bertoni discovered • The minor glycosides are considered to be less sweet, 30 – 80 Stevia rebaudiana in Paraguay in 1888. times sweeter than sugar • The plant was named in 1905 in honour of Paraguayan chemist Dr. Rebaudi. • 150 Stevia species have been identified but Stevia rebaudiana is the sweetest of all. Processing The Stevia Farming The native habitat- • latitude of 25° S in a subtropical region of northeastern Paraguay Two sweet products of stevia exist • between 500–1,500 m above sea level, • dried stevia leaf or powder • soil having a low phosphate content • Purified stevia glycoside powder (commercialized) • annual average temperature of 75°F, • average rainfall of about 55 in. per year Processing involves four basic steps: • Seedlings are transplanted in April or May (on the basis of • Dissolving the sweetener in boiling water or other solvent study in Japan) (Methanol) • Requires frequent irrigation • Ion-exchange, membrane technology for purification and • is a poor competitor with weeds, concentration • warm temperatures with minimal frost, and high light • Filtration with precipitation/coagulation intensities are required. • Crystallisation and drying. • Acidic soil pH (pH 4–5) favors rapid growth (80 cm). • Can be harvested in Summer and Autumn. Conclusion • Non-nutritive natural sweetener with high sweetness Use of Stevia products and medicinal value • Japan is the biggest user. • No food safety concern, appropriate for diabetes • Approved by USFDA as a food additive • Developed processing techniques • Can be used in most of the foods. • Preferably used in drinks • Potential high value cash crop for Nepalese agri-sector.