1. By Stephanie Wilson

2. How do you create a Transgenic
Mouse?
•Several methods but one most often used:
Transgenic Mouse Process Animation

3. First Method of Transgenic Mice Creation
• (basic steps) Standard Transgenic Approach:
1. Microinject fertilized mouse eggs with
transgene

4. Methods continued…
2. Injected eggs are implanted into a foster
mother

5. Methods continued…
3. Offspring are then born “Pups”; possible
founders (have the transgene)

6. Method Continued…
4. Potential founders (pups with the transgene) are
found using a PCR analysis and or southern blotting
(Black offspring = founders)
5. Founders can then be further analyzed for affects
of that transgene

7. Methods Continue…
Transgenic Mice Technology Part 1
Transgenic Mice Technology Part 2

8. Why create transgenic mice?
•Transgenic Mice have:
•Aided the study of Alchemies Disease
• Aided in study of pancreatic cancer
Transgenic Mice aids study of Pancreatic Cancer
•Acted as disease models
•Tested toxicants
•Produced specific proteins helpful in
biotechnology

9. Pros and Cons
• Pros
– Cheap
– Short generation time
– Defined genes
• Cons
– Ethical Concerns
• Are the animals treated with respect?
– Environmental Issues
• What if a genetically modified animal gets loose?
– Religious Views
• Do we have the right to act as God?

10. Animation And Picture Credits
• Experiments." Experiments. Web. 23 May 2012.
<http://student.biology.arizona.edu/honors2005/group07/Experiments.html>.
• "Transgenic Mice." Transgenic Mice. Web. 23 May 2012.
<http://www.accessexcellence.org/RC/VL/GG/transgenic.php>.
• Creating Transgenic Mice: On-Line Self-Study." Human Development. Professor Danton O'Day.
Web. <http://www.utm.utoronto.ca/~w3bio380/pdf/Creating%20Transgenic%20Mice.pdf>.
• "Transgenic Mouse Animation." Transgenic Mouse Animation. Web. 23 May 2012.
<http://spine.rutgers.edu/cellbio/assets/flash/transgen.htm>.
• Transgenic Animals. 13 June 2011. Web.
<http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/T/TransgenicAnimals.html>.
• "Fig002jme: Two Methods to Produce Transgenic Mice." Fig002jme: Two Methods to Produce
Transgenic Mice. Web. 23 May 2012.
<http://journals.cambridge.org/fulltext_content/ERM/ERM3_12/S1462399401002952sup00
4.htm>.
• Proneural. "The Science of Transgenic Technology Part 2 of 2." YouTube. YouTube, 25 Apr.
2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=EFNtTfhhQ70>.
• Proneural. "The Science of Transgenic Technology Part 1 of 2." YouTube. YouTube, 25 Apr.
2007. Web. 23 May 2012. <http://www.youtube.com/watch?v=ujZHrR1mro8>.