1. Diagnostic Products Corporation Beyond Reference Intervals: Interpretive Frameworks for Immunoassay A. Paul Durham Dir. Scientific Publications

12. Rio2003 Data from: Semjonow.

13. Data from: Semjonow. Rio2003 Mean and variance increase with age.

18. Insulin-like Growth Factor I (IGF-I) Soldin et al. Pediatric Reference Ranges, 4th Ed. AACC Press, 2003:125. Rio2003

19. AACC’s PRR4 — Typical Annotations Soldin et al. Pediatric Reference Ranges, 4th Ed. AACC Press, 2003:178, from T4 section. Hospitalized patients Hoffmann-like technique Regression transfer

20. Cortisol in Pregnancy, and Post Partum Gillian Lockitch Handbook of Diagnostic Biochemistry and Hematology in Normal Pregnancy. CRC Press, 1993.

29. Box and Whisker Box and Fishbone Rio2003 central 50%, 80%, 90%, 95% implementation- dependent “ outliers”

30. Busselton Study, 2002. Rio2003 Men: Calculated Free Testosterone (cFT)

31. Tromso Study Rio2003 Svartberg et al. JCEM 2003;88:3099. Publication’s use of means and parametric statistics directly, on data sets likely to be nongaussian, makes it unreliable to estimate month-related reference ranges by meta-analysis.

32. Elmlinger, 2002. IGF-I / IGFBP-3 Ratio, by Tanner Stage Rio2003

33. Bivariate Box-and-Fishbone Plots Log SHBG, on Body Mass Index Maastricht / Diepenbeek Postmenopausal Study, 2002. Central 95% Light Blue Patch Central 50% Blue Patch Central 80% Dark Blue Line

44. Interpretation via Two Distributions Data from: Stephan et al. Clin Chem 2002;48:1279. Density BPH, N=292 PCa, N=567 Likelihood Ratio Rio2003

48. Growth Chart “ The use of DS specific growth charts is important for diagnosis of associated diseases, such as coeliac disease and hypothyroidism, which may further impair growth.” Myrelid et al. Arch Dis Child 2002;87:97. Rio2003 Height, Boys Down Syndrome Children

49. National Height Velocity Standards Hermanussen et al. Horm Res 2002;58:71. Differences partly due to data analysis!

50. Elmlinger, 2002. Rio2003 IGF-I, in Children

52. sIgE Rio2003 Allergen-specific IgE assays: Concentrations translatable into “Class” scores. Decision Level 0.35 kU/L Detection Limits: A: 0.35 kU/L B: 0.10 kU/L No finite upper limit (dilutions).

53. ‘ Cumulative Distribution Analysis’ Plots “ CDA” plots: Krouwer. Clin Chem 1987;33:2305. sIgE assays compared to skin prick testing

54. “ Censored” ROC Plot ROC plot: receiver operating characteristic plot. Curves don’t extend to the corner.

55. Diagnostic Products Corporation www.DPCweb.com  technical documents [email_address] D922b Rio2003

60. Highest result from N=3 sample expected to fall at about 80th centile (Z=+0.85 SD), on average . Highest and lowest values expected to enclose central 95%, on average , only for N  28 & higher. Centile Z-score (SDI)