One page overview about how Mix&Go surface chemistry/coating works to attach fragile biomolecules to a variety of synthetic surfaces including beads of all sizes, glass slides, microtiter plates and biosensors

1. Mix&Go Surface Chemistry
An Introduction
Background
Damage-Free Protein Binding
Many tests in diagnostics and life
science
research
require
the
attachment
of
proteins,
e.g.
antibodies or antigens, to a synthetic
surface. A key objective in this
process is to attach proteins strongly
while maintaining their active tertiary
structure. This is a difficult challenge
as it requires strong interactions
between
the
relatively
fragile
proteins and a synthetic surface that
may not be biocompatible.
Since each chelation point created
by Mix&Go is too weak to damage
the protein, each protein can be
attached in its functional tertiary
form. Yet, the protein is attached
strongly to the synthetic surface due
to the multiple chelation points.
To make matters more difficult,
when designing a device or
consumable material, selection is
often driven by bulk properties and
not optimal surface characteristics.
Microtitre plates, for example, need
to be made from injection mouldable,
water insoluble plastics. The surface
properties of these plastics make it
necessary to denature and damage
the proteins to bind them to the
surface.
The binding of a protein to a
surface is not a static process:
micro-environmental stresses at the
surface can put strain on the bonds.
Another advantage of Mix&Go is that
chelation points can break and
reform releasing the stresses without
causing protein damage or the
protein being released from the
surface.
These attributes are:
•
Mix&Go acts like an all purpose
“glue” that is applicable to a
broad
variety
of
synthetic
surfaces and biomolecules
•
Proteins
immobilized
with
Mix&Go have minimal or no
protein damage
•
Mix&Go changes the underlying
surface to a common chelating
(binding) surface
•
Mix&Go is a water based
chemistry; once activated with
Mix&Go
synthetic
surfaces
remain active for years
•
Mix&Go facilitates very
protein binding kinetics
•
Mix&Go binds proteins very
strongly, binding strength is
comparable to the one between
biotin and streptavidin.
•
Different metal polymers, e.g.
length and shape give rise to
diverse binding characteristics.
Mix&Go Attributes
Mix&Go acts like “molecular
Velcro”. Its key attributes convey
technical and economic benefits,
that cannot be achieved with other
methods.
fast
The Mix&Go Approach
Mix&Go takes a novel approach to
attaching fragile proteins to a
synthetic surface. It relies on metal
ions chelating to electron donating
groups both on the protein and on
the chosen synthetic surface. Since,
individually, none of these chelation
points is strong enough to securely
anchor the protein to the surface a
polymer of metal ions is used. This
is an example of avidity or multicomponent interactions.
Although each chelation point is
relatively weak multiple metal ions in
polymeric form can form very strong
interactions with a variety of different
surfaces, e.g. glass/silica, metal
oxides, or polystyrene.
Fig. 1: Securely attaching a protein to a synthetic surface with only one metal
ion is impossible as the binding strength of one chelation point is too weak.
Multiple metal ions, however, provide multiple chelation points to both surface
and protein holding them together gently yet strongly without damaging the
protein.
Anteo Diagnostics, Ltd. p Unit 4, 26 Brandl Street p Brisbane Technology Park p Eight Mile Plains p QLD 4113, Australia
Overview V1.1 6/12

2. Mix&Go Overview
Mix&Go Benefits
Mix&Go Product Family
Mix&Go Sweet Spot
Mix&Go conveys the following
technical and economic benefits:
Mix&Go is a family of different
metal ions in polymeric form. The
members of this product family do
not have specificity to a particular
protein as biotin has to streptavidin.
Mix&Go binds to a “motif” or pattern
of electron donating species on the
surface of the protein and all
members of the Mix&Go product
family have a fair degree of flexibility
and redundancy in the selection of
proteins they can bind.
Mix&Go is a generic method of
protein immobilisation.
Its key
attribute is minimal protein damage
regardless of the type of surface
chosen.
The advantages of
Mix&Go are particularly relevant in
situations where:
• The available surface area for
binding antibodies is limited
(multiplexing, miniaturization)
•
Antibodies need to work on many
different surfaces
Different Mix&Go formulations with
various polymeric species, have
different
patterns
of
binding
strengths with different proteins. The
binding motifs are different for
different
metal
polymers
and
therefore optimum performance can
be achieved by selection of the
appropriate Mix&Go and by further
optimising the conditions for protein
binding.
•
The assay is developed on one
surface and will need to be
transferred to another surface for
commercialisation.
•
Creating a Mix&Go surface and
attaching proteins to it is generic,
easy and very fast (see Fig. 2)
•
Mix&Go surfaces have excellent
batch-to-batch uniformity as well
as reproducibility and consistency of performance across
different devices, formats, and
consumables.
•
Very fast kinetics allows for the
coupling to be completed in the
order of minutes. Improved time
to result is due to more functional
proteins per unit surface area.
•
Creating a Mix&Go surface and
attaching proteins to it are both
easily scalable processes.
•
Mix&Go saves as much as 90%
antibody at the same sensitivity
and dynamic range performance.
•
Mix&Go significantly improves
sensitivity and broadens dynamic
range in cases where surface
area for protein binding is limited.
This makes Mix&Go ideal for
miniaturization and multiplexing.
Fig. 2 Coating surfaces (here beads) with Mix&Go and attachment of proteins to
the Mix&Go surface are both fast and easy processes.
Anteo Dx Overview
Anteo Diagnostics is an Australian
company developing proprietary
surface coatings for applications in
the diagnostic and life sciences
industries.
The product family,
called Mix&Go™, enables the fast,
easy, and stable attachment of
biomolecules such as antibodies,
streptavidin and other proteins to a
variety
of
synthetic
surfaces
including beads, slides, and plates.
Anteo actively pursues commercial
opportunities across the healthcare,
biotech and diagnostics industries as
well as other sectors for its patented
technology.
Anteo Diagnostics,
founded in 2005, is headquartered in
Brisbane, Queensland, Australia and
is traded on the Australian Securities
Exchange (ASX:ADO).
For more information please see
our webpage: www.anteodx.com or
contact us at contact@anteodx.com
If you attach proteins to a surface we want to talk to you!
Anteo Diagnostics Ltd. p Unit 4, 26 Brandl Street p Brisbane Technology Park p Eight Mile Plains p QLD 4113, Australia
Overview V1.1 6/12