genetic engineering

1. Dr Arunima karkun

2.  Bacteriophages are viruses that infect
bacterial cells.
 Infected cells are used as hosts to replicate
the virus.
 E. coli phages used due to ease of culture
and quick regeneration

4.  It is used for cloning foreign genes with many
other applications
 In this process proteins and peptides are fused
to the Capsid(surface) of the phage
 The combination of the phage and peptide is
known as a Fusion Protein

5.  Different sets of genes are
inserted into the genomes
of multiple phages
 These separate phages will
only display one protein,
peptide, or antibody
 Collections of these phages
can comprise Libraries
 These Libraries are exposed
to selected targets and only
some phages will interact
with targets

6.  Recombinant DNA technology to
incorporate foreign cDNA of interest into
viral DNA.
 Spliced into gene for a coat protein so
the protein will be displayed on outside
of phage particles
 Unbound phages are washed away leaving
only those showing affinity for the
receptors
 Eluted phages showing specificity are
used to infect new host cells for
amplification.
 Cycle repeated 2-3 times for stepwise
selection of best binding sequence.

7.  The analysis process will be done where
phages can be propagated then
characterized with DNA sequencing.
 3 types of common phages used in phage
display are the M13 and F1
 Virus takes up a small amount of area
 Through using multiple Virions polypeptide
libraries can be created, and each phage
displays a random peptide

9.  Epitope mapping and mimicking
 Identification of new receptors & ligands
 Drug discovery
 Epitope discovery – new vaccines
 Creation of antibody libraries
 Organ targeting

10.  Proteins Isolated through phage display will
have a specific interactions with its target
 Whether this target is a antigen, protein, or
disease it allows a quick effective treatment
for it