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 Unprecedented heavy metals (HMs) in the environment have
become a dilemma for all living organisms including plants.
 key elements of these are chelating metals by forming
phytochelatins (PCs) or metallothioneins (MTs) ,proline (Pro) metal
complex at the intra- and intercellular level, which is followed by
the removal of HM ions from sensitive sites or vacuolar
sequestration of ligand-metal complex

 Bioactive-metals, based on their physicochemical properties,
are divided into two groups of redox-active metals such as Cr,
Cu, Mn, and Fe and non-redox active metals such as Cd, Ni,
Hg, Zn, and Al. The redox metals can directly generate
oxidative injury .
 DNA strand breakage, defragmentation of proteins, or cell
membrane and damage to photosynthetic pigments,


 It is well documented that Cr is a toxic agent for the
growth and development of plants.
 Causes of environmental pollution
 Cr is found in the forms of trivalent Cr3+ and
hexavalent Cr6+ species.
 The highest concentration of Cr occurs in the root
rather than other parts of plants.
 Al is known as an inhibitory element for the
growth of plants, especially in acidic soils with pH
values as low as 5 or 5.5
 The primary target of Al toxicity is roots of plants
the inhibition of root growth and change their color
to brown .Reduction of root respiration .
 Al toxicity stress negatively affects aerial parts of
plants, especially as a result of initial root damage
 Aluminum (Al)
 Mn2+ is the most stable and soluble form of
manganese in the soil environment However,
lower soil pH, less soil organic matter, and
decreased redox potential increase the
availability or toxicity of Mn2+ to plants.
 Mn toxicity are first visible in aerial
organs of plants.
 Manganese (Mn)
 Cu is an essential micronutrient that participates in many
vital physiological functions of plants including acting as a
catalyzer of redox reaction in mitochondria, chloroplasts, and
cytoplasm of cells or as an electron carrier during plant
respiration However, Cu becomes toxic when its
concentration in the tissue of plants rises above optimal
levels
 Chlorosis, necrosis, and leaf discoloration .
 Copper (Cu)

 Ni is a micronutrient that is required by both higher
and lower plants in very small amounts. divalent state
(Ni2+) is the most stable.
 The concentration level representing nickel toxicity in
plants varies greatly from 25 to 246 μg·g−1 dry weight
(DW) of plant tissue, depending on the plant species
and cultivars
Nickel (Ni)


 Plants possess a sophisticated and interrelated network of
defense strategies to avoid or tolerate HM intoxication.
Physical barriers are the first line of defense in plants against
metals. Some morphological structures like thick cuticle,
biologically active tissues like trichomes, and cell walls as
well as mycorrhizal symbiosis can act as barriers .
 Some of the biological molecules involved in cellular metal
detoxification can be multifunctional and have antiradical,
chelating, or antioxidant activities.
 Phytochelatin have a high affinity to bind to HMs when they
are at toxic levels Phytochelatins, as a pathway for metal
homeostasis and detoxification, have been identified in a wide
range of living organisms from yeast and fungi to many
different species of animals
 Cytosol is the place where PCs are manufactured and actively
shipped from there in the form of metal-phytochelatin
complexes of high molecular weight to vacuole as their final
destination.
 PCs can be both produced and accumulated in roots and aerial
organs.
Phytochelatins (PCs)

 MTs, found in a wide variety of eukaryotic
organisms including fungi, invertebrates,
mammals, and plants as well as some prokaryotes
 In addition to their role in HM detoxification

a constituent of cell wall, antioxidant, and
macromolecules stabilizer .
Response to tensions caused by a wide range of biotic and
abiotic stressors such as excessive salinity, drought,
increased solar ultraviolet (UV) radiation.
A number of mechanisms by which Pro increases the
resistance of plants to HM toxicity have been proposed

 Symbiotic mycorrhizal fungi such as AM form a
mutualistic symbiosis with roots of most vascular
plant species .
 They improve the mineral nutrition status of plants
and can also enhance their tolerance towards some
stresses and pollutants .
Stress ppt

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Stress ppt

  • 1.
  • 2.  Unprecedented heavy metals (HMs) in the environment have become a dilemma for all living organisms including plants.  key elements of these are chelating metals by forming phytochelatins (PCs) or metallothioneins (MTs) ,proline (Pro) metal complex at the intra- and intercellular level, which is followed by the removal of HM ions from sensitive sites or vacuolar sequestration of ligand-metal complex
  • 3.   Bioactive-metals, based on their physicochemical properties, are divided into two groups of redox-active metals such as Cr, Cu, Mn, and Fe and non-redox active metals such as Cd, Ni, Hg, Zn, and Al. The redox metals can directly generate oxidative injury .  DNA strand breakage, defragmentation of proteins, or cell membrane and damage to photosynthetic pigments,
  • 4.
  • 5.   It is well documented that Cr is a toxic agent for the growth and development of plants.  Causes of environmental pollution  Cr is found in the forms of trivalent Cr3+ and hexavalent Cr6+ species.  The highest concentration of Cr occurs in the root rather than other parts of plants.
  • 6.  Al is known as an inhibitory element for the growth of plants, especially in acidic soils with pH values as low as 5 or 5.5  The primary target of Al toxicity is roots of plants the inhibition of root growth and change their color to brown .Reduction of root respiration .  Al toxicity stress negatively affects aerial parts of plants, especially as a result of initial root damage  Aluminum (Al)
  • 7.  Mn2+ is the most stable and soluble form of manganese in the soil environment However, lower soil pH, less soil organic matter, and decreased redox potential increase the availability or toxicity of Mn2+ to plants.  Mn toxicity are first visible in aerial organs of plants.  Manganese (Mn)
  • 8.  Cu is an essential micronutrient that participates in many vital physiological functions of plants including acting as a catalyzer of redox reaction in mitochondria, chloroplasts, and cytoplasm of cells or as an electron carrier during plant respiration However, Cu becomes toxic when its concentration in the tissue of plants rises above optimal levels  Chlorosis, necrosis, and leaf discoloration .  Copper (Cu)
  • 9.   Ni is a micronutrient that is required by both higher and lower plants in very small amounts. divalent state (Ni2+) is the most stable.  The concentration level representing nickel toxicity in plants varies greatly from 25 to 246 μg·g−1 dry weight (DW) of plant tissue, depending on the plant species and cultivars Nickel (Ni)
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  • 12.   Plants possess a sophisticated and interrelated network of defense strategies to avoid or tolerate HM intoxication. Physical barriers are the first line of defense in plants against metals. Some morphological structures like thick cuticle, biologically active tissues like trichomes, and cell walls as well as mycorrhizal symbiosis can act as barriers .  Some of the biological molecules involved in cellular metal detoxification can be multifunctional and have antiradical, chelating, or antioxidant activities.
  • 13.  Phytochelatin have a high affinity to bind to HMs when they are at toxic levels Phytochelatins, as a pathway for metal homeostasis and detoxification, have been identified in a wide range of living organisms from yeast and fungi to many different species of animals  Cytosol is the place where PCs are manufactured and actively shipped from there in the form of metal-phytochelatin complexes of high molecular weight to vacuole as their final destination.  PCs can be both produced and accumulated in roots and aerial organs. Phytochelatins (PCs)
  • 14.   MTs, found in a wide variety of eukaryotic organisms including fungi, invertebrates, mammals, and plants as well as some prokaryotes  In addition to their role in HM detoxification
  • 15.  a constituent of cell wall, antioxidant, and macromolecules stabilizer . Response to tensions caused by a wide range of biotic and abiotic stressors such as excessive salinity, drought, increased solar ultraviolet (UV) radiation. A number of mechanisms by which Pro increases the resistance of plants to HM toxicity have been proposed
  • 16.   Symbiotic mycorrhizal fungi such as AM form a mutualistic symbiosis with roots of most vascular plant species .  They improve the mineral nutrition status of plants and can also enhance their tolerance towards some stresses and pollutants .