PPSC Chemistry Lecturer Preparation (Test # 22)- Malik Xufyan

Malik Xufyan WhatsApp # 0313-7355727
PPSC CHEMISTRY CLASS
Test Session
CH: Chromatography
Test # 22
For more WhatsApp # 0313-7355727
Topics:
1- Introduction and types of Chromatography
2- Thin Layer Chromatography and Applications
3. Coloum Chromatography and Applications
Introduction and types of Chromatography
1. Chromatography is a physical method that is used to separate and analyse
__________
a) Simple mixtures
b) Complex mixtures
c) Viscous mixtures
d) Metals
2. In which type of chromatography, the stationary phase held in a narrow tube and the
mobile phase is forced through it under pressure?
a) Column chromatography
b) Planar chromatography
c) Liquid chromatography
d) Gas chromatography
3. In chromatography, the stationary phase can be ___________ supported on a solid.
a) Solid or liquid
b) Liquid or gas
c) Solid only
d) Liquid only
4. In chromatography, which of the following can the mobile phase be made of?
a) Solid or liquid
b) Liquid or gas

c) Gas only
d) Liquid only
5. Which of the following cannot be used as an adsorbent in Column adsorption
chromatography?
a) Magnesium oxide
b) Silica gel
c) Activated alumina
d) Potassium permanganate
6. Which of the following types of chromatography involves the separation of substances
in a mixture over a 0.2mm thick layer of an adsorbent?
a) Gas liquid
b) Column
c) Thin layer
d) Paper
7. Chromatography cannot be used to purify volatile substances.
a) True
b) False
8. In Column chromatography, the stationary phase is made of _________ and the
mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas
9. Chromatography cannot be used to separate delicate products.
a) True
b) False
10. In Thin layer chromatography, the stationary phase is made of _________ and the
mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas
11. In which of the following type of paper, chromatography does the mobile phase
move horizontally over a circular sheet of paper?

a) Ascending paper chromatography
b) Descending paper chromatography
c) Radial paper chromatography
d) Ascending – descending chromatography
12. Liquid chromatography can be performed in which of the following ways?
a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces
13. Gas chromatography can be performed in which of the following ways?
a) Only in columns
b) Only on plane surfaces
c) Either in columns or on plane surfaces
d) Neither in columns nor on plane surfaces
14. In Gas-liquid phase chromatography, the stationary phase is composed of
_________ and the mobile phase is made of _________
a) Solid, liquid
b) Liquid, liquid
c) Liquid, gas
d) Solid, gas
15. Which of the following types of chromatography involves the process, where the
mobile phase moves through the stationary phase by the influence of gravity or
capillary action?
a) Column Chromatography
b) High Pressure Liquid Chromatography
c) Gas Chromatography
d) Planar Chromatography
16. Which of the following steps takes place after injection of feed in Column
chromatography?
a) Detection of components
b) Separation in the column
c) Elution from the column
d) Collection of eluted component

17. Components with a strong attraction to the support move more slowly than
components with weak attraction.
a) True
b) False
18. What happens during the ‘elution from the column’ phase in chromatography?
a) Components with greatest affinity elute first
b) Components with least affinity elute first
c) Components elute in a random manner
d) Components elute according to their concentration in the mixture
19. In chromatogram, the position of peaks on the time axis can be used to determine
which of the following?
a) Components of the sample
b) Amount of component in the sample
c) Column efficiency
d) Column resolution
20. In chromatogram, the area under the peak can be used to determine which of the
following?
a) Components of the sample
b) Amount of component in the sample
c) Column efficiency
d) Column resolution
21. The stationary phase could be a viscous liquid coated over a surface of solid
particles.
a) True
b) False
22. Given below is a diagram of separation of two components of a mixture in a
chromatographic column. From the diagram, infer which component has lesser affinity
to the stationary phase.

a) W
b) X
c) Y
d) Z
23. Using Chromatogram as detector in Chromatography, a graph is obtained between
____________ and time.
a) Quantity
b) Density
c) Concentration
d) Specific gravity
24. In older analytical methods, which of the following methods were used to allow
movement of the mobile phase?
a) Pumps
b) Pressure
c) Gravity
d) Blowing air into the column
25. Given below is the diagram of ‘Process of chromatographic column’. Identify the
unmarked component in the process.

a) Reservoir
b) Collection tanks
c) Microprocessor
d) Detector
26. How is the molar concentration of solute in stationary phase related to molar
concentration of solute in the mobile phase?
a) Directly proportional
b) Inversely proportional
c) Equal
d) Not related
27. If the value of the distribution constant ‘k’ is one, then what could be inferred about
the distribution of solute?
a) Its distribution in stationary phase is greater
b) Its distribution in mobile phase is greater
c) It is equally distributed in stationary and mobile phase
d) It is distributed in a random manner
28. The time taken by the analyte after sample injection to reach the detector is called
_________
a) Dead time
b) Solute migration rate
c) Adjusted retention time
d) Retention time

29. The time required for a molecule of the mobile phase to pass through the column is
called ___________
a) Dead time
b) Solute migration rate
c) Adjusted retention time
d) Retention time
30. Adjusted retention time is the remaining retention time after subtracting
__________ from ___________
a) Solute migration rate and retention time
b) Retention time and solute migration rate
c) Dead time and retention time
d) Retention time and dead time
31. Which of the following is the volume of mobile phase required to make a solute band
move from the point of injection through the column to the detector?
a) Dead volume
b) Retention volume
c) Void volume
d) Adjusted retention volume
32. Adjusted retention volume is the remaining retention volume after subtracting
___________ from _____________
a) Solute migration rate and retention volume
b) Retention volume and solute migration rate
c) Dead volume and retention volume
d) Retention volume and dead volume
33. Which of the following is defined as the ratio of moles of solute in stationary phase
to the moles of solute in the mobile phase?
a) Distribution constant
b) Volumetric phase ratio
c) Retention factor
d) Total porosity
34. Which of the following is the ratio of the interstitial volume of packing to the volume
of its total mass?
a) Distribution constant
b) Volumetric phase ratio

c) Retention factor
d) Total porosity
35. Which of the following is the ratio of the length of column packing to dead time?
a) Average linear rate of solute migration
b) Average linear rate of mobile migration
c) Relative migration rate
d) Selectivity factor
36. Which of the following is the ratio of a length of column packing to retention time?
a) Average linear rate of solute migration
b) Average linear rate of mobile migration
c) Relative migration rate
d) Selectivity factor
37. Retention distance is the distance between point of injection and minimum peak in
the recorder or computer generated chart.
a) True
b) False
38. Retention volume can be obtained by finding the product of which of the following
parameters?
a) Dead time and total porosity
b) Retention time and volumetric flow rate
c) Adjusted retention time and volumetric flow rate
d) Retention time and total porosity.
39. Retention factor is also known as the capacitance factor.
a) True
b) False
40. What must be the value of the selectivity factor?
a) Equal to 1
b) Less than 1
c) Greater than 1
d) Greater than 0
Thin Layer Chromatography
41. The principle on which thin layer chromatography is based is that the ____________
a) Different compounds are absorbed on an absorbent to different degrees

b) Different compounds are absorbed on an absorbent to same degrees
c) Different compounds are adsorbed on an adsorbent to different degrees
d) Different compounds are absorbed on an absorbent to same degrees
42. The size of a thin layer of adsorbent is about ____________
a) 0.1 mm
b) 0.2 mm
c) 0.3 mm
d) 0.4 mm
43. The chromaplate or thin layer chromatography plate is made up of ____________
a) Glass
b) Wood
c) Fibre
d) Metal
44. The eluant filled in the closed jar is ____________
a) Mixture of gases
b) Mixture of a liquid and a gas
c) Mixture of solids
d) Mixture of liquids
45. Select the incorrect statement from the following options.
a) The spots of colorless compounds are invisible to the eyes
b) These spots can be detected by putting the plate under ultraviolet light
c) These spots can be detected by placing the plate in a covered jar containing iodine crystals
d) None of the mentioned
46. The relative adsorption of each component of the mixture is expressed in terms of its
____________
a) Acceleration factor
b) Retardation factor
c) Both acceleration and retardation factor
d) None of the mentioned
47. Select the correct statement from the following.
a) Paper chromatography is a type of partition chromatography
b) A special quality paper is used in paper chromatography
c) Chromatography paper contains water trapped in it, which acts as stationary phase
d) All of the mentioned

48. Amino acids detected by spraying the plate with ninhydrin solution is an example of
____________
b) Thin layer chromatography
c) Paper chromatography
d) Liquid chromatography
49. Paper chromatography is based on continuous differential partitioning of
components of a mixture between stationary and mobile phases.
a) True
b) False
50. Retardation factor is the ratio of ____________
a) Distance moved by substance from base line to distance moved by the solvent from base
line
b) Distance moved by solvent from base line to distance moved by the substance from base
line
c) Distance moved by substance from top line to distance moved by the solvent from top line
d) Distance moved by solvent from top line to distance moved by the substance from top line
Column Chromatography
51. Column chromatography is based on the principle of _______________
a) Ion-exchange
b) Exclusion principle
c) Differential adsorption
d) Absorption
52. Arrange the following compounds in order of their increasing adsorption tendencies.
a) Cellulose >> starch >> calcium carbonate >> alumina
b) Cellulose >> starch >> alumina >> charcoal
c) Charcoal >> cellulose >> alumina >> starch
d) Calcium carbonate >>; alumina >> starch >> cellulose
53. What is the factor responsible for the separation in column chromatography?
a) Polarity differences between the solvent
b) Polarity differences between the solute

c) Polarity indifference between the solvent
d) Polarity indifference between the solute
54. Select the correct statement from the following options.
a) The lesser the polarity of solute, more strongly it will be adsorbed on a polar surface
b) The greater the polarity of solute, more weakly it will be adsorbed on a polar surface
c) The greater the polarity of solute, more strongly it will be adsorbed on a polar surface
d) All of the mentioned option
55. The correct order of increasing strength of adsorption is ____________
a) Alkanes >> Esters >> Aldehydes >> Phenols >> Ketones
b) Aldehydes >> Phenols >> Ketones >> Esters >> Alkanes
c) Aldehydes >> Ketones >> Esters >> Alkanes >> Phenols
d) Alkanes >> Esters >> Ketones >> Aldehydes >> Phenols
56. The components of the mixture in column chromatography are eluted in order of
____________
a) Increasing polarity and decreasing distribution ratio
b) Increasing polarity and increasing distribution ratio
c) Decreasing polarity and increasing distribution ratio
d) Decreasing polarity and decreasing distribution ratio
57. The elution power of a solvent is determined by ____________
a) Its overall polarity
b) The polarity of the stationary phase
c) The nature of the sample components
58. Which of the following is separated through column chromatography?
a) Chlorophyll and carotenoids
b) Inorganic cations or complexes
c) Sugar derivatives
d) Amino acids formed by hydrolysis of a protein molecule
59. The mixture of petroleum ether and benzene is used in the elution ratio of
____________
a) 1 : 2
b) 1 : 5
c) 1 : 9
d) 1 : 12

60. Chloroform fraction is eluted from the column by passing chloroform through the
column which acts as ____________
a) Eluter
b) Eluant
c) Elution
d) None of the mentioned option
Applications
61. What are the uses of partition chromatography?
a) Separation of amino acids
b) Analysis of closely related aliphatic alcohols
c) Separation of sugar derivatives
62. Which type of chromatography is used for the structural analysis?
b) Paper chromatography
c) Partition chromatography
d) Affinity chromatography
63. Which of the following is not done using column chromatography?
a) The identification of unknown compounds
b) The determination of homogeneity of chemical substances
c) Separation of inorganic cations or complexes
d) Separation of geometric isomers
64. Affinity chromatography is used for the analysis and isolation of ____________
a) Insoluble starch substances
b) Enzyme tyrosinase
c) Antibodies bound with a covalently-attached antigen on cellulose column
65. Purification of enzymes and proteins is done using ____________

a) Affinity chromatography
b) Liquid chromatography
c) Column chromatography
d) Thin layer chromatography
66. Which of the following is not an application of high performance liquid
chromatography?
a) Analysis of proteins, drugs and explosives
b) Separation of pharmaceutical drugs
c) Elimination of undesirable substances from blood
d) Separation of lipids, fatty acids and steroids
67. The checking of purity of samples is the application of thin layer chromatography.
a) True
b) False
68. The analysis and separation of industrial products like soap and synthetic detergents
is done using ____________
a) Thin layer chromatography
b) Gas chromatography
c) Ion exchange chromatography
d) Partition chromatography
69. Which of the following is the application of ion exchange chromatography?
a) The softening of hard water
b) The demineralisation of water
c) The separation and determination of anions
70. The quantitative analysis is done using ____________
a) Ion exchange chromatography
b) Thin layer chromatography
c) Gas chromatography
d) Liquid chromatography

PPSC Chemistry Lecturer Preparation (Test # 22)- Malik Xufyan

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