CorDynamics conducts thrombosis experiments using small and large animals to examine pro-thrombotic activity from a safety perspective. Mouse models are used where ferric chloride is applied to the carotid artery to cause endothelial injury and monitor blood flow changes over time. Tests are done with various concentrations of ferric chloride to establish a pro-thrombotic window, showing that reducing the concentration reduces thrombosis as evidenced by increased occlusion times and incidence of non-occlusion.

1. Shifting Paradigms: Examining
Pro-Thrombotic Activity from a
Safety Perspective

2. CorDynamics conducts thrombosis experiments – using small and
large animals
Mouse ferric chloride model of arterial thrombosis
1) instrumentation to monitor carotid artery flow
- monitor artery flow prior to and after FeCl3
- FeCl3 migrates to lumen, results in endothelial injury
2) administer test article prior to FeCl3
-monitor for development of thrombus
-use real-time monitor of blood flow, time to occlusion
Validation
- reduced concentrations of FeCl3, vessel vulnerability present

3. Effects of various concentrations FeCl3 on carotid artery blood flow
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mL/min
Time (minutes)
Concetration-dependent effects of Ferric Chloride on
Carotid Arterial Thrombosis in CD-1 Mice
Carotid Artery Flow
Vehicle (PBS, 3.5% FeCl3- Lot SZBE1900V) (n=5)
Vehicle (PBS, 3.0% FeCl3- Lot SZBE1900V) (n=10)
Vehicle (PBS, 2.8% FeCl3- Lot SZBE1900V) (n=10)
Vehicle (PBS, 2.5% FeCl3- Lot SZBE1900V) (n=10)
FeCl3

4. Reducing FeCl3 reduces thrombosis – prothrombotic window opens
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3.5% FeCl3
Lot SZBE1900V
3.0% FeCl3
Lot SZBE1900V
2.8% FeCl3
Lot SZBE1900V
2.5% FeCl3
Lot SZBE1900V
ProThrombotic
OcclusionTime(min)
Incidenceofnon–Occlusion(%)
Concetration-dependent effects of Ferric Chloride on
Carotid Arterial Thrombosis in CD-1 Mice
Occlusion time
Incidence of non-occlusion
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0/7
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