Indo-Nippon Medical Trading Co is proud to be the exclusive distributor of all Vitrification products by Cryo Tech Lab in India.Cryo Tech India will be now be involved in the field of human in vitro fertilization.Cryo Tech India has developed vitrification for cryopreservation products for all procedures and the individual stages of embryo development. Cryo Tech India will facilitate rapid availability of all Cryo Tech Lab products in a controlled environment.Also IVf vitrification for cryopreservation products and disposables for infertility treatment with in vitro fertilization and assisted reproduction technologies. Cryo Tech Lab products for IVF Lab ,assisted reproduction technologies clinics: Cryo Tech Lab products for IVF clinics for high performance vitrification to assist the Artificial Reproductive Centers in the world in the preservation of oocytes and embryos with more than 99% of survival, increasing their efficiency and success. Vitrification Kit Vitri-Solution [Vitrification Solution] Solutions of the Cryotec vitrification for cryopreservation Cryotec method Vitri-Plate Warm-plate Cryocontainer-Cryotec Warming Kit Warm-Solution Cryo Tech Lab Vitrification cryopreservation products are ready-to-use and especially designed to meet specific requirements during fertilisation and culturing of early embryos,fertilisation of human oocytes,culture of embryos,embryo transfer,micromanipulation,oocyte,cryopreservation. To Buy IVF Vitrification cryopreservation products,Please visit Cryo Tech India online by visiting Website: www.cryotechindia.com or simply email at info.cryotech.india@gmail.com Or CONTACT AT: Indo-Nippon Medical Trading Co 304, 3rd Floor, B Wing, 36, Turner Road, Bandra (West), Mumbai - 400050, India Phone: +91 22 61996500 ; +91 22 26552000 www.cryotechindia.com

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Cryo Tech Lab was established to capacitate and widespread our technologies on cryop
high performance vitrification to assist the Artificial Reproductive Centers in the world in t

2. oocytes and embryos with more than 99% of survival, increasing their efficiency a
developments have been shared with other international researchers achieving amazing res
oocytes and embryos at all stages and the highest standards of pregnancy and live birth.
most efficient method and is leading the world in cryopreservation including ovarian tissue c
News
It’s like magic, but science. 100% Survival Vitrification!
The Final Best Vitrification protocol was established.
Highest Survival & Highest Safety Method is now being provided ...

3. CryoTech Products
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Cryo Tec
1,600 1 Cryotec
VC
1 vial of 1ml
Equilibration Solution
(ES)
2 vials of 1ml
Vitrification
101 12,800 Vitrification Solution
Kit
(VS)
4 Cryotecs
3 Vitriplates
1 vial of 1.8ml
Warming Solution
(TS)
1 vial of 0.5ml
Diluent Solution (DS)
Warming Kit 102 6,400
1 vial of 1.0ml
Washing Solution
(WS)
1 Warm plate

4. CryoTech India Products
2 vials of 1.8ml
Equilibration Solution
Vitri-
Solution (ES)
110 40,000
(10 times 4 vials of 1.8ml
set)
Vitrification Solution
(VS)
5 vials of 4.2ml
Warming Solution
(TS)
Warm- 1 vial of 1.8ml
Solution 205 20,000
(5 times set) Diluent Solution (DS)
2 vials of 1.8ml
Washing Solution
(WS)
Vitri Plate 1150 1 Vitriplate
Warm Plate 1150 1 Warm plate
*12.5% VAT will be additional
*Delivery charges will be extra at actuals
Vitrification Kit
• 1 vial of 1ml Equilibration Solution (ES)
• 2 vials of 1ml Vitrification Solution (VS)
• 4 Cryotecs
• 3 Vitriplates
Vitri-Solution (10 times set)
• 2 vials of 1.8ml Equilibration Solution (ES)
• 4 vials of 1.8ml Vitrification Solution (VS)
Warming Kit
• 1 vial of 1.8ml Warming Solution (TS)
• 1 vial of 0.5ml Diluent Solution (DS)
• 1 vial of 1.0ml Washing Solution (WS)

5. CryoTech India Products
• 1 Warm plate
Warm-Solution (5 times set)
• 5 vials of 4.2ml Warming Solution (TS)
• 1 vial of 1.8ml Diluent Solution (DS)
• 2 vials of 1.8ml Washing Solution (WS)

6. For Oocytes and Embryos
VITRIFICATION KIT (101)
Contents of the Kit
-Equilibration Solution (ES): 1 vial of 1.0ml. Quality Control Tests:
-Vitrification Solution (VS): 2 vials of 1.0ml. This Lot Nº JIHA0115 (All Solutions)
-4 Cryotecs: 1 cryotec can contain more than one oocyte/embryo
(recommend 3-4 maximum for oocytes and 4-cells embryos, and Successfully passed the following controls:
one for blastocyst for single embryo transfer). ・ Sterility : Sterility test .
-3 Vitri Plates with 3 wells each. ・ Endotoxin by ES methodology (Each component).
・ Efficiency: survival of 50/50 Mouse embryos and Porcine oocytes.
Instructions:
Preparation Storage and stability
-The whole process should be performed under room temperature Solutions and kits can be transported under the room temperature,
(25-27ºC). and then must be keep in the fridge at 2-8ºC until the expiration
-Fill a nitrogen container. date.
-Compare the thickness of the zona pellucida with the periviteline
space, and take note for oocyte. Composition
-Important: Use a Pasteur pipet with the right diameter for oocyte, -Modified HEPES Buffered MEM
embryo (140-150 µm) and Blastocyst (160-200 µm). -Hydroxy Propyl Cellulose
-Ethylene Glycol
Equilibration of oocytes and embryos -Dimethyl Sulfoxide
1. Fill the Vitri Plate with 300 µl of ES in the 1º well, and 300 µl of VS -Endotoxin free Trehalose
in the 2º and 3º well.
2. Put the oocyte/embryo on the surface of ES in the 1º well. References
3. The oocyte/embryo will sink and begin to shrink, and gradually • Kuwayama M. Highly efficient vitrification for cryopreservation of human
returns to the original size (maximum 15 min for oocyte and oocytes and embryos: The CryoTop method. Theriogenology 67, 73-80,
blastocysts, and 12 min for other stages of embryos). 2007.
• Cobo A, Kuwayama M. Comparison of concomitant outcome schieved with
fresh and cryopreserved donor oocytes vitrified by the Cryotop method.
Vitrification
Fertil Steril. J89(6): 1657-64, 2007.
Attention: The following steps must be made in no less than 25 sec • Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop
and a maximum time of 90 sec. vitrification of human oocytes results in high survival rate and healthy
deliveries. Reproductive BioMedicine Online 14, 5-667, 2007.
4. Transfer the oocyte/embryo to the half depth of the 2° well with • Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology
VS. (Not with minimum volume of ES at the first step) The 65(1), 236-44, 2006.
oocyte/embryo immediately floats to the surface of VS while • Kuwayama M. Highly efficient vitrification method for cryopreservation of
human oocytes. Reproductive BioMedicine Online 11:300-308, 2005.
washed.
• Ushijima J, Kuwayama M. High survival rate of bovine oocytes matured in
5. After washing the inside wall of the pipette with fresh VS media, vitro following vitrification. J Reprod Dev. 50:685-96, 2004.
take only the oocyte/embryo and transfer it to the bottom of the • Fukui Y, Kuwayama M. Effect of cryodevice type and donor’s sexal maturity
well. Wait until the oocyte/embryo floating stops in VS. on vitrification of minke whale oocytes at germinal vesicle stage. Zygote 12,
6. Transfer the oocyte/embryo to the middle depth of the 3° well 333-338, 2004.
with VS, and mix the media by pipette around for 5 times. • Hochi S, Kuwayama M. Improved Survival of Vitrified in vivo-derived porcine
7. Take only the oocyte/embryo at the top end in the pipette, and put embryos. J. Reprod. Develop. 50, 481-486, 2004.
• Esaki R, Kuwayama M. Cryopreservation of porcine embryos derived from in
it on the end of the cryotec seat with minimum volume of VS.
vitri- matured oocytes. Biology of Reproduction. 71, 432-437, 2004.
8. Immediately submerge the Cryotech into liquid nitrogen.
9. Place the cap, and store it in a nitrogen tank.
Product for in vitro use only.
Please stay Cryotec in liquid nitrogen at all times.
www.cryotechlab.com cryotechlab@gmail.com

7. For Oocytes and Embryos
WARMING KIT (102)
Contents of the Kit
-Warming Solution (TS): 1 vial of 1.8 ml. Quality Control Tests
-Diluent Solution (DS): 1 vial of 0.5ml. This Lot Nº JIHA0115 (All Solutions)
-Washing Solution (WS): 1 vial of 1ml. Successfully passed the following controls:
-1 Warm-plates with 4 wells each. ・ Sterility : Sterility test .
・ Endotoxin by ES methodology (Each component).
・ Efficiency: survival of 50/50 Mouse embryos and Porcine oocytes.
Instructions
Preparation
Storage and stability
-The whole process should be made under room temperature (25-
Solutions and kits can be transported under the room temperature, and
27ºC).
then must be keep in the fridge at 2-8ºC until the expiration date.
-Important: Use a Pasteur pipet with the right diameter for oocyte,
embryo (140-150 µm) and Blastocyst (160-200 µm).
Composition
-Place the Warm-plate and TS vial (with rid) in the incubator at 37°C 3
-Modified HEPES Buffered MEM
hours before the use (overnight storage is preferable).
-Hydroxy Propyl Cellulose
-Expose DS and WS vials to room temperature air at least 1 hour before
-Endotoxin free Trehalose
the use.
-Take the Warm-plate and TS vial out of the incubator, and expel the
vial to the first square well.
References
• Kuwayama M. Highly efficient vitrification for cryopreservation of human
Warming and dilution of CPAs oocytes and embryos: The CryoTop method. Theriogenology 67, 73-80, 2007.
1. Quickly (within 1 sec) put the Cryotec into the 1º square well with TS, • Cobo A, Kuwayama M. Comparison of concomitant outcome schieved with fresh
and wait for 1 min. and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril.
2. While waiting, fill the second well with 300 µl of DS. J89(6): 1657-64, 2007.
3. Aspirate the oocyte/embryo and 3 mm long of TS into the pipette, • Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop
and expel them most slowly to the bottom of the second well (DS). vitrification of human oocytes results in high survival rate and healthy
deliveries. Reproductive BioMedicine Online 14, 5-667, 2007.
And wait for 3 min.
• Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology
4. While waiting, fill the third (WS1) and fourth wells (WS2) with 300 µl 65(1), 236-44, 2006.
of WS each. • Kuwayama M. Highly efficient vitrification method for cryopreservation of
5. Aspirate the oocyte/embryo and 3 mm long of DS into the pipette, human oocytes. Reproductive BioMedicine Online 11:300-308, 2005.
and expel them slowly to the bottom of the third well (WS1), and • Ushijima J, Kuwayama M. High survival rate of bovine oocytes matured in vitro
wait for 5 min. following vitrification. J Reprod Dev. 50:685-96, 2004.
6. Give a survival judgment at the end of this step if the shrunk • Fukui Y, Kuwayama M. Effect of cryodevice type and donor’s sexal maturity on
vitrification of minke whale oocytes at germinal vesicle stage. Zygote 12, 333-
oocyte/embryo to be recovered or not.
338, 2004.
7. Put the oocyte/embryo on the surface of the fourth well (WS2). • Hochi S, Kuwayama M. Improved Survival of Vitrified in vivo-derived porcine
When they sink and reach to the bottom, put them again on the embryos. J. Reprod. Develop. 50, 481-486, 2004.
surface of the same WS2 to wash for 2 times in total. • Esaki R, Kuwayama M. Cryopreservation of porcine embryos derived from in
8. Put the oocyte/embryo in the droplet of the culture media for the vitri- matured oocytes. Biology of Reproduction. 71, 432-437, 2004.
recovery for ICSI and ET.
Product for in vitro use only.
Note: 2 to 4 hours of culture for oocytes, and 3 hours for embryos.
www.cryotechlab.com cryotechlab@gmail.com

8. For Oocytes and Embryos
VITRIFICATION SOLUTION SET (110) : For 10 times Uses
Contents of Vitrification solutions set Quality Control Tests:
This Lot Nº JIHA0115 (All Solutions)
-Equilibration Solution (ES): 2 vials of 1.6ml.
-Vitrification Solution (VS): 4 vials of 1.6ml. Successfully passed the following controls:
・ Sterility : Sterility test .
Instructions: ・ Endotoxin by ES methodology (Each component).
Preparation ・ Efficiency: survival of 50/50 Mouse embryos and Porcine oocytes.
-The whole process should be performed under room temperature
(25-27ºC). Storage and stability
-Fill a nitrogen container. Solutions and kits can be transported under the room temperature,
-Compare the thickness of the zona pellucida with the periviteline and then must be keep in the fridge at 2-8ºC until the expiration
space, and take note for oocyte. date.
-Important: Use a Pasteur pipet with the right diameter for oocyte,
embryo (140-150 µm) and Blastocyst (160-200 µm). Composition
-Modified HEPES Buffered MEM
Equilibration of oocytes and embryos -Hydroxy Propyl Cellulose
1. Fill the Vitri Plate with 300 µl of ES in the 1º well, and 300 µl of VS -Ethylene Glycol
in the 2º and 3º well. -Dimethyl Sulfoxide
2. Put the oocyte/embryo on the surface of ES in the 1º well. -Endotoxin free Trehalose
3. The oocyte/embryo will sink and begin to shrink, and gradually
returns to the original size (maximum 15 min for oocyte and References
blastocysts, and 12 min for other stages of embryos). • Kuwayama M. Highly efficient vitrification for cryopreservation of human
oocytes and embryos: The CryoTop method. Theriogenology 67, 73-80,
Vitrification 2007.
Attention: The following steps must be made in no less than 25 sec • Cobo A, Kuwayama M. Comparison of concomitant outcome schieved with
fresh and cryopreserved donor oocytes vitrified by the Cryotop method.
and a maximum time of 90 sec.
Fertil Steril. J89(6): 1657-64, 2007.
• Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop
4. Transfer the oocyte/embryo to the half depth of the 2° well with vitrification of human oocytes results in high survival rate and healthy
VS. (Not with minimum volume of ES at the first step) The deliveries. Reproductive BioMedicine Online 14, 5-667, 2007.
oocyte/embryo immediately floats to the surface of VS while • Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology
washed. 65(1), 236-44, 2006.
5. After washing the inside wall of the pipette with fresh VS media, • Kuwayama M. Highly efficient vitrification method for cryopreservation of
human oocytes. Reproductive BioMedicine Online 11:300-308, 2005.
take only the oocyte/embryo and transfer it to the bottom of the
• Ushijima J, Kuwayama M. High survival rate of bovine oocytes matured in
well. Wait until the oocyte/embryo floating stops in VS. vitro following vitrification. J Reprod Dev. 50:685-96, 2004.
6. Transfer the oocyte/embryo to the middle depth of the 3° well • Fukui Y, Kuwayama M. Effect of cryodevice type and donor’s sexal maturity
with VS, and mix the media by pipette around for 5 times. on vitrification of minke whale oocytes at germinal vesicle stage. Zygote 12,
7. Take only the oocyte/embryo at the top end in the pipette, and put 333-338, 2004.
it on the end of the cryotec seat with minimum volume of VS. • Hochi S, Kuwayama M. Improved Survival of Vitrified in vivo-derived porcine
8. Immediately submerge the Cryotech into liquid nitrogen. embryos. J. Reprod. Develop. 50, 481-486, 2004.
• Esaki R, Kuwayama M. Cryopreservation of porcine embryos derived from in
9. Place the cap, and store it in a nitrogen tank.
vitri- matured oocytes. Biology of Reproduction. 71, 432-437, 2004.
Please stay Cryotec in liquid nitrogen at all times.
Product for in vitro use only.
www.cryotechlab.com cryotechlab@gmail.com

9. For Oocytes and Embryos
WARMING SOLUTION SET (205): For 5 times Uses
Contents of Warming solutions set Quality Control Tests
-Warming Solution (TS): 5 vials of 1.8 ml. This Lot Nº JIHA0115 (All Solutions)
-Diluent Solution (DS): 1 vial of 1.8ml. Successfully passed the following controls:
-Washing Solution (WS): 2 vials of 1.8ml. ・ Sterility : Sterility test .
・ Endotoxin by ES methodology (Each component).
Instructions ・ Efficiency: survival of 50/50 Mouse embryos and Porcine oocytes.
Preparation
-The whole process should be made under room temperature (25- Storage and stability
27ºC). Solutions and kits can be transported under the room temperature, and
-Important: Use a Pasteur pipet with the right diameter for oocyte, then must be keep in the fridge at 2-8ºC until the expiration date.
embryo (140-150 µm) and Blastocyst (160-200 µm).
-Place the Warm-plate and TS vial (with rid) in the incubator at 37°C 3 Composition
hours before the use (overnight storage is preferable). -Modified HEPES Buffered MEM
-Expose DS and WS vials to room temperature air at least 1 hour before -Hydroxy Propyl Cellulose
the use. -Endotoxin free Trehalose
-Take the Warm-plate and TS vial out of the incubator, and expel the
vial to the first square well.
References
Warming and dilution of CPAs • Kuwayama M. Highly efficient vitrification for cryopreservation of human
1. Quickly (within 1 sec) put the Cryotec into the 1º square well with TS, oocytes and embryos: The CryoTop method. Theriogenology 67, 73-80, 2007.
and wait for 1 min. • Cobo A, Kuwayama M. Comparison of concomitant outcome schieved with fresh
2. While waiting, fill the second well with 300 µl of DS. and cryopreserved donor oocytes vitrified by the Cryotop method. Fertil Steril.
J89(6): 1657-64, 2007.
3. Aspirate the oocyte/embryo and 3 mm long of TS into the pipette,
• Antinori M, Licata E, Dani G, Cerusico F, Versaci C, Antinori S. Cryotop
and expel them most slowly to the bottom of the second well (DS). vitrification of human oocytes results in high survival rate and healthy
And wait for 3 min. deliveries. Reproductive BioMedicine Online 14, 5-667, 2007.
4. While waiting, fill the third (WS1) and fourth wells (WS2) with 300 µl • Vajta G, Kuwayama M. Improving cryopreservation systems. Theriogenology
of WS each. 65(1), 236-44, 2006.
5. Aspirate the oocyte/embryo and 3 mm long of DS into the pipette, • Kuwayama M. Highly efficient vitrification method for cryopreservation of
and expel them slowly to the bottom of the third well (WS1), and human oocytes. Reproductive BioMedicine Online 11:300-308, 2005.
• Ushijima J, Kuwayama M. High survival rate of bovine oocytes matured in vitro
wait for 5 min.
following vitrification. J Reprod Dev. 50:685-96, 2004.
6. Give a survival judgment at the end of this step if the shrunk • Fukui Y, Kuwayama M. Effect of cryodevice type and donor’s sexal maturity on
oocyte/embryo to be recovered or not. vitrification of minke whale oocytes at germinal vesicle stage. Zygote 12, 333-
7. Put the oocyte/embryo on the surface of the fourth well (WS2). 338, 2004.
When they sink and reach to the bottom, put them again on the • Hochi S, Kuwayama M. Improved Survival of Vitrified in vivo-derived porcine
surface of the same WS2 to wash for 2 times in total. embryos. J. Reprod. Develop. 50, 481-486, 2004.
8. Put the oocyte/embryo in the droplet of the culture media for the • Esaki R, Kuwayama M. Cryopreservation of porcine embryos derived from in
vitri- matured oocytes. Biology of Reproduction. 71, 432-437, 2004.
recovery for ICSI and ET.
Note: 2 to 4 hours of culture for oocytes, and 3 hours for embryos.
Product for in vitro use only.
www.cryotechlab.com cryotechlab@gmail.com