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Labs & assignments
 Lab activities will parallel lecture material (to all extent
possible) and handout materials will be used as
appropriate.
 All lab assignments must be submitted via
Blackboard one week after the assigned dates unless
otherwise noted by the instructor.
 No duplicated Lab!!!!!!!!!
Biol 205: Lab 1
Ecological Data
&
Descriptive Statistics
Dr. Davenport
Objectives
 Why and what is statistics?
 What is data?
 Basic principle of statistics -- relationship
between (statistical) population and sample?
 Descriptive Statistics
 Assignment and Questions
Why statistic?
How to draw the intelligent judgment in the presence of
uncertainty?
Statistics
is a branch of applied mathematics that helps us
to make intelligent judgements and informed
decisions in the presence of uncertainty and
variation.
• Useful in the planning of experiments and
studies that will result in meaningful data.
• Provides a set of tools to extract and
understand information resulting from
experiments.
Data is :
 collection of facts from which conclusions may be
drawn
 representation of facts, concepts, or instructions in a
formal manner suitable for communication,
interpretation, or processing by human beings or by
computers.
 formal representation of raw material from which
information is constructed via processing or
interpretation.
Why you need data?
Basic principle of statistics
 The data is very important to present, summary
and interpret the ecological phenomena.
 However, it usually is impossible or impractical
to monitor the entire habitat or obtain
measurements of all the organisms in a given
area.
 So most time, only part of the population will
be sampled when you acquire a set of data.
8
Population
 The entire group of individuals is called the
population.
 For example, a researcher may be interested in
the relation between class size (variable 1) and
academic performance (variable 2) for a
population of third-grade children.
9
Sample
 Usually populations are so large that a researcher
cannot examine the entire group. Therefore, a
sample (subset of population) is selected to
represent the population in a research study.
The goal is to use the results obtained from the
sample to infer information about the
population.
Basic principle of statistics
Summary
Population: the set of all measurements
of interest.
Sample: a subset of measurements of
interest to the investigator.
Population Sample
Statistics
Selecting Samples
 Sample should be taken at a random order.
 Why?
 Random sampling implies that each measurement in
the population has an equal opportunity of being
selected as part of your sample.
 Otherwise, your samples could be biased.
Sampling Replication
 Why do we need replication?
 Single measurement generally is insufficient to draw
a conclusion about a population.
Definitions
Descriptive Statistics: basic tools for
summarizing and presenting numerical
data.
Low Birth Weight Data
Variable Abbreviation
Identification Code ID
Low Birth Weight (0 = Birth Weight >= 2500g, LOW
1 = Birth Weight < 2500g)
Age of the Mother in Years AGE
Weight in Pounds at the Last Menstrual Period LWT
Race (1 = White, 2 = Black, 3 = Other) RACE
Smoking Status During Pregnancy (1 = Yes, 0 = No) SMOKE
History of Premature Labor (0 = None, 1 = One, etc.) PTL
History of Hypertension (1 = Yes, 0 = No) HT
Presence of Uterine Irritability (1 = Yes, 0 = No) UI
Number of Physician Visits During the First Trimester FTV
(0 = None, 1 = One, 2 = Two, etc.)
Birth Weight in Grams BWT
Low Birth Weight Data
ID LOW AGE LWT RACE SMOKE PTL HT UI FTV BWT
85 0 19 182 2 0 0 0 1 0 2523
86 0 33 155 3 0 0 0 0 3 2551
87 0 20 105 1 1 0 0 0 1 2557
88 0 21 108 1 1 0 0 1 2 2594
89 0 18 107 1 1 0 0 1 0 2600
91 0 21 124 3 0 0 0 0 0 2622
92 0 22 118 1 0 0 0 0 1 2637
76 1 20 105 3 0 0 0 0 3 2450
77 1 26 190 1 1 0 0 0 0 2466
78 1 14 101 3 1 1 0 0 0 2466
79 1 28 95 1 1 0 0 0 2 2466
81 1 14 100 3 0 0 0 0 2 2495
82 1 23 94 3 1 0 0 0 0 2495
83 1 17 142 2 0 0 1 0 0 2495
84 1 21 130 1 1 0 1 0 3 2495
Hosmer and Lemeshow (2000) Applied Logistic Regression:
2nd Edition; John Wiley & Sons
N=189
Data Presentation
Three ways to summarize, or
describe data:
1. Tables
2. Graphics
3. Basic Summary Statistics
Tabulations
Tables are used to describe qualitative data. The
tables simply present the counts, or frequencies,
observed in each category of a variable of interest.
Race
White
Black
Other
Count
96
26
67
%
51
14
35
Tabulations
None
One
Two
Three
Four or More
Visits Count Percent
100
47
30
7
5
52.9
24.9
15.9
3.7
2.6
Physician Visits During the 1st Trimester
No Visits One Visit Two Visits Three Visits Four or More
020406080100
Bar Chart
Physician Visits During First Trimester
NoVisits
OneVisit
Two
Visits
Three Visits
Four or More
Number of Physcian VisitsPie Chart
Physician Visits During First Trimester
Summary Statistics
Measures of Center (Central Tendency)
Mean
Median
Mode
Measures of Spread (Variability)
Range
Variance
Standard Deviation
Mean
The mean of a data set is the average of all the data values.
 If the data are from a sample, the mean is denoted by
 If the data are from a population, the mean is denoted
by “mu”.
x
x
n
i

 
 x
N
i
x
Measures of Center
Mean (average): sum of sampled values
divided by the number of samples taken.
X =
1
n
Xi
i=1
n
å
n = sample size
Xi = sampled value
 = symbol for summation
 = population mean
X = sample mean
Measures of Center
Example:
30, 26, 26, 36, 48, 50, 16, 31, 22, 27, 23, 35, 52, 28, 37
 
1
1 1
30 26 ... 37 32.47
15
n
i
i
X X
n 
     
Note: The mean is sensitive to extreme values.
30, 26, 26, 36, 48, 50, 16, 31, 22, 27, 23, 35, 52, 28, 37, 113
37.50X 
How do extreme values affect the mean?
( 1)
2
2 2
[ ]
[ ] [ 1]
if n is odd
if n is even
2
n
n n
x
X x x




  


Measures of Center
Median: the value of a set of measurement
that falls in the middle position when the
data are ordered from smallest to largest.
Measures of Center
16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52
N = 15 is odd, so the 8th value is the median:
The 8th valueWhy 8? (15 + 1)/2 = 8
16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52, 113
How do extreme values affect the median?
Now N=16, so the average of the 8th and 9th value is
the median, which is 30.5 ... not much different from
the original data!
Measures of Center
Mode: the value of a set of measurements
that occurs most frequently.
In our example data, the mode is 26.
16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52
26 is the mode
Fact: For data that is
symmetric and unimodal, the
mean, median and mode are
similar.
Measures of Spread
Range: the difference between the largest
and smallest sample measurements.
In our example, the range is 36.
16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52
Note: Two data sets may have
the same range, but very
different shape and variability.
R = 52-16 = 36
Measures of Spread
Sum of squared deviations from the mean,
which is referred to simply as the sum of
squares (SS)
_
SS = ∑(Xi - X)2
Measures of Spread
Variance (s2): the sum of the squares of the
deviations divided by the sample size
minus one.
Standard Deviation (s): the square root of
the variance.
2
2
( )
1
ix x
s
n




2
s s
Measures of Spread
 Degree of freedom (DF):
 DF = n-1
Measures of Spread
A computationally more convenient formula
to calculate the variance:
 
2
2
2 2
2
1 1
i
i
i
x
xx nx ns
n n

 
 


Measures of Spread
The variance and standard deviation for our
example are:
16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52
2
510.8
22.6
s
s


Normal Distribution
https://en.wikipedia.org/wiki/Normal_distribution#/media/File:Normal_Distribution_PDF.svg
Lab 1: Assignment
As a fishery scientist working for NOAA,
you did lots research on the strip bass (rockfish)
population in the Chesapeake Bay. In one of your
studies, you gathered data about the age structure
for rockfish population in the Chesapeake Bay,
and you need to do some statistical analysis before
you can present your data to the public.
The fish samples you collected were in 3 age
groups: age1 (1 year old); age2 (2 year old), and age
3 (3 years old).
Lab 1: Questions
1. What is statistical population (N)? What is
sample (n)? What is the relationship between
statistical population and sample? What
information does the sample (n) infer about the
statistical population (N)?
2. Write the definition (formulas) for variance and
standard deviation
3. Draw a bar chart and a pie chart about the
number of the fishes from different age groups
(the age structure about your sample).
Lab 1: Questions (continued)
4. What is the average weight of the fishes in your
entire sample?
5. What are the average weights of the fishes in
different age groups (age1, age2, and age3)?
6. What is the median weight of the fishes for age
1 group? And, What is the median weight of the
fishes for age 3 group?
7. What is the range of the weight for the fishes at
age 2 group?
Lab 1: Questions (continued)
8. Calculate the variance of the weight of the fishes
at age 2 group.
9. Calculate the standard deviation of the weight
for fishes at age 1 group.

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Lab 1 intro

  • 1. Labs & assignments  Lab activities will parallel lecture material (to all extent possible) and handout materials will be used as appropriate.  All lab assignments must be submitted via Blackboard one week after the assigned dates unless otherwise noted by the instructor.  No duplicated Lab!!!!!!!!!
  • 2. Biol 205: Lab 1 Ecological Data & Descriptive Statistics Dr. Davenport
  • 3. Objectives  Why and what is statistics?  What is data?  Basic principle of statistics -- relationship between (statistical) population and sample?  Descriptive Statistics  Assignment and Questions
  • 4. Why statistic? How to draw the intelligent judgment in the presence of uncertainty?
  • 5. Statistics is a branch of applied mathematics that helps us to make intelligent judgements and informed decisions in the presence of uncertainty and variation. • Useful in the planning of experiments and studies that will result in meaningful data. • Provides a set of tools to extract and understand information resulting from experiments.
  • 6. Data is :  collection of facts from which conclusions may be drawn  representation of facts, concepts, or instructions in a formal manner suitable for communication, interpretation, or processing by human beings or by computers.  formal representation of raw material from which information is constructed via processing or interpretation.
  • 7. Why you need data? Basic principle of statistics  The data is very important to present, summary and interpret the ecological phenomena.  However, it usually is impossible or impractical to monitor the entire habitat or obtain measurements of all the organisms in a given area.  So most time, only part of the population will be sampled when you acquire a set of data.
  • 8. 8 Population  The entire group of individuals is called the population.  For example, a researcher may be interested in the relation between class size (variable 1) and academic performance (variable 2) for a population of third-grade children.
  • 9. 9 Sample  Usually populations are so large that a researcher cannot examine the entire group. Therefore, a sample (subset of population) is selected to represent the population in a research study. The goal is to use the results obtained from the sample to infer information about the population.
  • 10. Basic principle of statistics
  • 11. Summary Population: the set of all measurements of interest. Sample: a subset of measurements of interest to the investigator. Population Sample Statistics
  • 12. Selecting Samples  Sample should be taken at a random order.  Why?  Random sampling implies that each measurement in the population has an equal opportunity of being selected as part of your sample.  Otherwise, your samples could be biased.
  • 13. Sampling Replication  Why do we need replication?  Single measurement generally is insufficient to draw a conclusion about a population.
  • 14. Definitions Descriptive Statistics: basic tools for summarizing and presenting numerical data.
  • 15. Low Birth Weight Data Variable Abbreviation Identification Code ID Low Birth Weight (0 = Birth Weight >= 2500g, LOW 1 = Birth Weight < 2500g) Age of the Mother in Years AGE Weight in Pounds at the Last Menstrual Period LWT Race (1 = White, 2 = Black, 3 = Other) RACE Smoking Status During Pregnancy (1 = Yes, 0 = No) SMOKE History of Premature Labor (0 = None, 1 = One, etc.) PTL History of Hypertension (1 = Yes, 0 = No) HT Presence of Uterine Irritability (1 = Yes, 0 = No) UI Number of Physician Visits During the First Trimester FTV (0 = None, 1 = One, 2 = Two, etc.) Birth Weight in Grams BWT
  • 16. Low Birth Weight Data ID LOW AGE LWT RACE SMOKE PTL HT UI FTV BWT 85 0 19 182 2 0 0 0 1 0 2523 86 0 33 155 3 0 0 0 0 3 2551 87 0 20 105 1 1 0 0 0 1 2557 88 0 21 108 1 1 0 0 1 2 2594 89 0 18 107 1 1 0 0 1 0 2600 91 0 21 124 3 0 0 0 0 0 2622 92 0 22 118 1 0 0 0 0 1 2637 76 1 20 105 3 0 0 0 0 3 2450 77 1 26 190 1 1 0 0 0 0 2466 78 1 14 101 3 1 1 0 0 0 2466 79 1 28 95 1 1 0 0 0 2 2466 81 1 14 100 3 0 0 0 0 2 2495 82 1 23 94 3 1 0 0 0 0 2495 83 1 17 142 2 0 0 1 0 0 2495 84 1 21 130 1 1 0 1 0 3 2495 Hosmer and Lemeshow (2000) Applied Logistic Regression: 2nd Edition; John Wiley & Sons N=189
  • 17. Data Presentation Three ways to summarize, or describe data: 1. Tables 2. Graphics 3. Basic Summary Statistics
  • 18. Tabulations Tables are used to describe qualitative data. The tables simply present the counts, or frequencies, observed in each category of a variable of interest. Race White Black Other Count 96 26 67 % 51 14 35
  • 19. Tabulations None One Two Three Four or More Visits Count Percent 100 47 30 7 5 52.9 24.9 15.9 3.7 2.6 Physician Visits During the 1st Trimester
  • 20. No Visits One Visit Two Visits Three Visits Four or More 020406080100 Bar Chart Physician Visits During First Trimester
  • 21. NoVisits OneVisit Two Visits Three Visits Four or More Number of Physcian VisitsPie Chart Physician Visits During First Trimester
  • 22. Summary Statistics Measures of Center (Central Tendency) Mean Median Mode Measures of Spread (Variability) Range Variance Standard Deviation
  • 23. Mean The mean of a data set is the average of all the data values.  If the data are from a sample, the mean is denoted by  If the data are from a population, the mean is denoted by “mu”. x x n i     x N i x
  • 24. Measures of Center Mean (average): sum of sampled values divided by the number of samples taken. X = 1 n Xi i=1 n å n = sample size Xi = sampled value  = symbol for summation  = population mean X = sample mean
  • 25. Measures of Center Example: 30, 26, 26, 36, 48, 50, 16, 31, 22, 27, 23, 35, 52, 28, 37   1 1 1 30 26 ... 37 32.47 15 n i i X X n        Note: The mean is sensitive to extreme values. 30, 26, 26, 36, 48, 50, 16, 31, 22, 27, 23, 35, 52, 28, 37, 113 37.50X  How do extreme values affect the mean?
  • 26. ( 1) 2 2 2 [ ] [ ] [ 1] if n is odd if n is even 2 n n n x X x x          Measures of Center Median: the value of a set of measurement that falls in the middle position when the data are ordered from smallest to largest.
  • 27. Measures of Center 16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52 N = 15 is odd, so the 8th value is the median: The 8th valueWhy 8? (15 + 1)/2 = 8 16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52, 113 How do extreme values affect the median? Now N=16, so the average of the 8th and 9th value is the median, which is 30.5 ... not much different from the original data!
  • 28. Measures of Center Mode: the value of a set of measurements that occurs most frequently. In our example data, the mode is 26. 16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52 26 is the mode Fact: For data that is symmetric and unimodal, the mean, median and mode are similar.
  • 29. Measures of Spread Range: the difference between the largest and smallest sample measurements. In our example, the range is 36. 16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52 Note: Two data sets may have the same range, but very different shape and variability. R = 52-16 = 36
  • 30. Measures of Spread Sum of squared deviations from the mean, which is referred to simply as the sum of squares (SS) _ SS = ∑(Xi - X)2
  • 31. Measures of Spread Variance (s2): the sum of the squares of the deviations divided by the sample size minus one. Standard Deviation (s): the square root of the variance. 2 2 ( ) 1 ix x s n     2 s s
  • 32. Measures of Spread  Degree of freedom (DF):  DF = n-1
  • 33. Measures of Spread A computationally more convenient formula to calculate the variance:   2 2 2 2 2 1 1 i i i x xx nx ns n n       
  • 34. Measures of Spread The variance and standard deviation for our example are: 16, 22, 23, 26, 26, 27, 28, 30, 31, 35, 36, 37, 48, 50, 52 2 510.8 22.6 s s  
  • 36. Lab 1: Assignment As a fishery scientist working for NOAA, you did lots research on the strip bass (rockfish) population in the Chesapeake Bay. In one of your studies, you gathered data about the age structure for rockfish population in the Chesapeake Bay, and you need to do some statistical analysis before you can present your data to the public. The fish samples you collected were in 3 age groups: age1 (1 year old); age2 (2 year old), and age 3 (3 years old).
  • 37. Lab 1: Questions 1. What is statistical population (N)? What is sample (n)? What is the relationship between statistical population and sample? What information does the sample (n) infer about the statistical population (N)? 2. Write the definition (formulas) for variance and standard deviation 3. Draw a bar chart and a pie chart about the number of the fishes from different age groups (the age structure about your sample).
  • 38. Lab 1: Questions (continued) 4. What is the average weight of the fishes in your entire sample? 5. What are the average weights of the fishes in different age groups (age1, age2, and age3)? 6. What is the median weight of the fishes for age 1 group? And, What is the median weight of the fishes for age 3 group? 7. What is the range of the weight for the fishes at age 2 group?
  • 39. Lab 1: Questions (continued) 8. Calculate the variance of the weight of the fishes at age 2 group. 9. Calculate the standard deviation of the weight for fishes at age 1 group.