Starke Mais TM-Ertragsverbesserung durch endophytische Samen-Impfung zweier Maissorten, Strong maize yield improvement by maize seed inoculation with endophytic mircoaorganism. Starke Ertragswirkung endophytischer Impfung TM bei 2 Maissorten, Q Naveed, Muhammad et al 2014. Boku Glashaus Versuch in Tulln 2011,Grafik Layout HUMER

1. Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Maisertragsteigerung durch endophytische Samenimpfung
Ertragsreaktion zweier Maissorten auf 2 Endophyten und Trockenheit
Grafik/Layout: Johann HUMER, Austria,SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert, 2016okt22
Boku Glashaus Versuch
in Tulln 2011
Mais TM-Ertrag g je Gefäß auf 572 cm² in 3 Monaten
24
18
27
22
32
26
31
29
36
31
34
32
17
19
21
23
25
27
29
31
33
35
37
M normal bewässert M Trockenstress K normal bewässert K Trockenstress
Maissorte
MAZURKA
Maissorte
KALEO
+46%
+45%
Kontrolle
unbehandelt
ohne Impfung
Burkholderia
phytofirmans
PsJN
Enterobacter
sp. FD17

2. Endophytische Samenimpfung erhöht Maisertrag
Endophyten hemmen Ertragsabfall bei Wassermangel
Q: Naveed, Muhammad, et al. "Increased drought stress resilience of maize through endophytic colonization by Burkholderia phytofirmans PsJN and Enterobacter sp. FD17." Environmental and Experimental
Botany 97 (2014): 30-39. Surface-disinfected seeds were incubated in bacterial suspension [prepared as (108–109 CFU mL–1)] for 2 h. Three inoculated seeds (10^^8 bacteria per seed) were sown in pots
withdiameter 27 cm andheight 25 cm containing 15 kg of soil and thinned to one plant after one week of germination. May to July 2011 in the greenhouse. The average maximum temperature was 20.6–27.6
◦C day and 10.7–15.7 ◦C night. The photoperiod was 16 h light and 8 h dark. Doses of N, P, and K fertilizers (160-100-60 kg/ ha). Drought stress was applied by stopping irrigation after 45 days of planting.
Boku Glashaus Versuch in Tulln 2011
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert
Maisorten >> Mazurka Mazurka Kaleo Kaleo
g TM/Gefäß normal bewässert Trockenstress normal bewässert Trockenstress
Unbehandelt 24,07 18,40 26,63 21,70
FD17 32,00 26,03 30,70 28,67
PsJN 35,60 30,57 33,98 31,60
Endophytische Mikroorganismen im Test: Enterobacter sp. FD17::gusA10, Burkholderia phytofirmans strain: PsJN::gusA10
Unbeh.100% 100% 100% 100% 100%
FD17 133% 141% 115% 132%
PsJN 148% 166% 128% 146%

3. Maisertragsteigerung durch endophytische Samenimpfung in TM
Ertragsreaktion zweier Maissorten auf Endophyten und Trockenheit
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert, 2016okt2
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011
Mais TM-Ertrag g je Gefäß auf 572 cm² in 3 Monaten
24
32
36
18
26
31
27
31
34
22
29
32
UNBEHANDELT, OHNE IMPFUNG ENTEROBACTER SP. FD17 BURKHOLDERIA PHYTOFIRMANS PSJN
M normal bewässert
M Trockenstress
K normal bewässert
K Trockenstress
Mazurka
Kaleo
Kaleo
Mazurka
Trockenstress
= hier keine
Bewässerung
nach 45 Tagen
Bewässerung
Mai bis Juli
3 Monate
90 Tage
Mais TM-Ertrag g je Gefäß auf
572 cm² in 3 Monaten
Endophytische Samenimpf-Stämme

4. Relative Maisertragsteigerung durch endophytische Samenimpfung
Trotz Bewässerungsstop: Maisertrag höher als ohne Endophyten
Boku Glashaus Versuch in Tulln 2011
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert
100%
133%
148%
76%
108%
127%
70%
80%
90%
100%
110%
120%
130%
140%
150%
160%
ohne Impfung 100% Enterobacter sp. FD17 Burkholderia phytof: PsJN
regelm. Glashausbewässerung Mai bis Juli
Bewässerungsstop 45 Tage nach Anbau
Endophytische Samenimpf-Stämme
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011Maissorte
MAZURKA
+51%

5. Relative Maisertragsteigerung durch endophytische Samenimpfung
Trotz Bewässerungsstop: Maisertrag höher als ohne Endophyten
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011Maissorte
MAZURKA
+66% zu ohne
Impfung
100%
115%
128%
81%
108%
119%
70%
80%
90%
100%
110%
120%
130%
140%
ohne Impfung 100% Enterobacter sp. FD17 Burkholderia phytof: PsJN
regelm. Glashausbewässerung Mai bis Juli
Bewässerungsstop 45 Tage nach Anbau
Endophytische Samenimpf-Stämme
Maissorte
Kaleo
+37%

6. Relative Maisertragsteigerung durch endophytische Samenimpfung
Trotz Bewässerungsstop: Maisertrag höher als ohne Endophyten
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011
+46% zu ohne
Impfung
100%
124%
138%
79%
108%
123%
70%
80%
90%
100%
110%
120%
130%
140%
ohne Impfung 100% Enterobacter sp. FD17 Burkholderia phytof: PsJN
regelm. Glashausbewässerung Mai bis Juli
Bewässerungsstop 45 Tage nach Anbau
Endophytische Samenimpf-Stämme
Maissortenmittel
MAZURKA
KALEO
+44%

7. Relative Maisertragsteigerung durch endophytische Samenimpfung
Trotz Bewässerungsstop: Maisertrag höher als ohne Endophyten
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011Maissortenmittel
MAZURKA
KALEO
100%
126%
80%
114%
70%
80%
90%
100%
110%
120%
130%
140%
ohne Impfung 100% Mittel Endophyten
regelm. Glashausbewässerung Mai bis Juli
Bewässerungsstop 45 Tage nach Anbau
Endophytische Samenimpf-Stämme
Maissorten + Endophyten Mittel
+34%

8. Maisertragsteigerung durch endophytische Samenimpfung, g TM/Topf
Trotz Bewässerungsstop: Maisertrag höher als ohne Endophyten
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011
25
33
20
29
19
21
23
25
27
29
31
33
35
ohne Impfung 100% Mittel Endophyten
regelm. Glashausbewässerung Mai bis Juli
Bewässerungsstop 45 Tage nach Anbau
Maissortenmittel
MAZURKA
KALEO
Mais TM-Ertrag g je Gefäß
auf 572 cm²
in 3 Monaten
Endophytische Mikroorganismen im Test:
Enterobacter sp. FD17::gusA10, Burkholderia phytofirmans strain: PsJN::gusA10
Maissorten + Endophyten Mittel

9. Maisertragsteigerung durch endophytische Samenimpfung in TM/ha
Trotz Bewässerungsstop: Maisertrag höher als ohne Endophyten
Grafik/Layout: Johann HUMER, Austria, SRVP – Science results visible presented – Wissenschaft anschaulich präsentiert, 2016okt2
Q: Naveed, Muhammad, et al. "Increased drought
stress resilience of maize through endophytic
colonization by Burkholderia phytofirmans PsJN and
Enterobacter sp. FD17." Environmental and
Experimental Botany 97 (2014): 30-39.
Surface-disinfected seeds were incubated in bacterial
suspension [prepared as (108–109 CFU mL–1)] for 2
h. Three inoculated seeds (10^8 bacteria per seed)
were sown in pots withdiameter 27 cm andheight 25
cm containing 15 kg of soil and thinned to one plant
after one week of germination. May to July 2011 in
the greenhouse. The average maximum temperature
was 20.6–27.6 ◦C day and 10.7–15.7 ◦C night. The
photoperiod was 16 h light and 8 h dark. Doses of N,
P, and K fertilizers (160-100-60 kg/ ha). Drought
stress was applied by stopping irrigation after 45 days
of planting.
Boku Glashaus Versuch
in Tulln 2011
4428
5776
3502
5103
3400
3900
4400
4900
5400
5900
ohne Samenimpfung Mittel Endophytenimpfung
regelm. Glashausbewässerung Mai bis Juli
Bewässerungsstop 45 Tage nach Anbau
Mais TM-Ertrag kg/ha
in 3 Monaten auf
572 cm² Basis
Endophytische Mikroorganismen im Test:
Enterobacter sp. FD17::gusA10, Burkholderia phytofirmans strain: PsJN::gusA10
Maissortenmittel
MAZURKA
KALEO
Österr. Feld Maiserträge:
10-20 t TM/ha