1.
Simultaneous mapping of multiple proteins
in heart using matrix-assisted laser
desorption/ionization imaging mass
spectrometry
Ken Takahashi
Department of Cardiovascular Physiology
Graduate School of Medicine, Dentistry and Pharmaceutical Sciences
Okayama University, Japan

2.
Background
• Expression and localization of proteins in
tissue determines anatomy and physiology,
and thus provides a clue to pathology.
• However, it is strenuous to identify the
expression and localization of multiple targets
using conventional immunostaining technique.

3.
MALDI-IMS
Int J Oncol, 2015. 46(3): p. 893-906

4.
ResultsHeart(n=2)
4 mm
Testis(n=2)

5.
Results
No. Accession Protein
MW
[kDa]
1 MYH6 Myosin-6 OS=Mus musculus 223.4
2 TNNT2 Troponin T, cardiac muscle 35.8
3 ACTC Actin, alpha cardiac muscle 1 42.0
4 MYL3 Myosin light chain 3 22.4
5 MYH8 Myosin-8 OS=Mus musculus 222.6
6 ACON Aconitate hydratase, mitochondrial 85.4
7 MYPC3 Myosin-binding protein C, cardiac-type 140.5
8 HBA Hemoglobin subunit alpha 15.1
9 G3P Glyceraldehyde-3-phosphate dehydrogenase 35.8
10 TPM1 Tropomyosin alpha-1 chain 32.7
11 TITIN Titin 3904.1
12 ACTG Actin, cytoplasmic 2 41.8
13 HBB1 Hemoglobin subunit beta-1 15.8
14 KCRS Creatine kinase, sarcomeric mitochondrial 47.4
15 ETFA Electron transfer flavoprotein subunit alpha, mitochondrial 35.0
16 HBB2 Hemoglobin subunit beta-2 15.9
17 ATPA ATP synthase subunit alpha, mitochondrial 59.7
18 MDHM Malate dehydrogenase, mitochondrial 35.6
19 TNNI3 Troponin I, cardiac muscle 24.2
20 KCRM Creatine kinase M-type 43.0
21 ALDOA Fructose-bisphosphate aldolase A 39.3
22 CRIP2 Cysteine-rich protein 2 22.7
23 MYG Myoglobin 17.1
24 THIM 3-ketoacyl-CoA thiolase, mitochondrial 41.8
25 IDHP Isocitrate dehydrogenase [NADP], mitochondrial 50.9
26 ATPB ATP synthase subunit beta, mitochondrial 56.3
27 COQ9 Ubiquinone biosynthesis protein COQ9, mitochondrial 35.1
28 MLRV Myosin regulatory light chain 2, ventricular/cardiac muscle isoform 18.9
29 ATP5J ATP synthase-coupling factor 6, mitochondrial 12.5
30 CSRP3 Cysteine and glycine-rich protein 3 20.9
31 UCRI Cytochrome b-c1 complex subunit Rieske, mitochondrial 29.3
32 MYH3 Myosin-3 223.7
No. Accession Protein
MW
[kDa]
33 COX5A Cytochrome c oxidase subunit 5A, mitochondrial 16.1
34 YBOX1 Nuclease-sensitive element-binding protein 1 35.7
35 QCR7 Cytochrome b-c1 complex subunit 7 13.5
36 MDHC Malate dehydrogenase, cytoplasmic 36.5
37 ACTBL Beta-actin-like protein 2 42.0
38 QCR2 Cytochrome b-c1 complex subunit 2, mitochondrial precursor 48.2
39 ETFB Electron transfer flavoprotein subunit beta 27.6
40 LDHB L-lactate dehydrogenase B chain 36.5
41 DESM Desmin OS=Mus musculus 53.5
42 HSP7C Heat shock cognate 71 kDa protein 70.8
43 ECI1 Enoyl-CoA delta isomerase 1, mitochondrial 32.2
44 AT2A2 Sarcoplasmic/endoplasmic reticulum calcium ATPase 2 114.8
45 ECHA Trifunctional enzyme subunit alpha, mitochondrial 82.6
46 THIL Acetyl-CoA acetyltransferase, mitochondrial 44.8
47 VINC Vinculin 116.6
48 CISY Citrate synthase, mitochondrial 51.7
49 EF1A2 Elongation factor 1-alpha 2 50.4
50 MYL1 Myosin light chain 1/3, skeletal muscle isoform 20.6
51 ACADM Medium-chain specific acyl-CoA dehydrogenase, mitochondrial 46.5
52 ACTN2 Alpha-actinin-2 103.8
53 NDUS6 NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial 13.0
54 CYC Cytochrome c, somatic 11.6
55 SCOT1 Succinyl-CoA:3-ketoacid coenzyme A transferase 1, mitochondrial 56.0
56 AT1A1 Sodium/potassium-transporting ATPase subunit alpha-1 112.9
57 ACTB Actin, cytoplasmic 1 41.7
58 YBOX1 Nuclease-sensitive element-binding protein 1 35.7
59 CALR Calreticulin 48.0
60 PRM2 Protamine-2 13.6
61 HSP72 Heat shock-related 70 kDa protein 2 69.6
62 RL18 60S ribosomal protein L18 21.6
63 SP17 Sperm surface protein Sp17 17.3
64 ALDOA Fructose-bisphosphate aldolase A 39.3

6.
Conclusion
MALDI imaging may provide a new platform for
analysis of pathology and subsequent
application to diagnosis of heart diseases.

7.
Acknowledgment
This work was supported by JSPS KAKENHI
Grant Number JP16K01356