10. Outcome of embryonic PCV2-infection Surgical ET HB 6 dpins PCV2/mock Inspection of ovaries (follicles/CL) Euthanasia D14 post ET Collection & analysis of embryos
11. Outcome of embryonic PCV2-infection Sow n° A B C D E F G Embryos at transfer n° status 26 mock 26 mock 20 PCV2 23 PCV2 20 PCV2 18 PCV2 29 PCV2 Ovaries CL CL CL CL follicles follicles follicles Embryos at euthanasia n° 19 17 13 3 0 0 0 normal 19 15 7 0 0 0 0 degenerated 0 2 6 3 0 0 0 mock normal embryo PCV2 degenerated embryo
12. Outcome of embryonic PCV2-infection Sow n° A B C D E F G Embryos at transfer n° status 26 mock 26 mock 20 PCV2 23 PCV2 20 PCV2 18 PCV2 29 PCV2 Ovaries CL CL CL CL follicles follicles follicles Embryos at euthanasia n° 19 17 13 3 0 0 0 normal 19 15 7 0 0 0 0 degenerated 0 2 6 3 0 0 0 0 0 0 4 6 3 IHC-PCV2
13. Outcome of embryonic PCV2-infection Sow n° A B C D E F G Embryos at transfer n° status 26 mock 26 mock 20 PCV2 23 PCV2 20 PCV2 18 PCV2 29 PCV2 Embryos at euthanasia Ovaries CL CL CL CL follicles follicles follicles n° 19 17 13 3 0 0 0 normal 19 15 7 0 0 0 0 degenerated 0 2 6 3 0 0 0 0 0 0 4 6 3 PCV2 infection of embryos leads to • embryonic death • return to oestrus of sow (regular/irregular) • both PCV2a and PCV2b IHC-PCV2
36. PEV Inoculated in fetus 8 at 59 days of gestation Hysterectomy at 113 days of gestation 7 living fetuses
37. CSFV Inoculation of CSFV (low virulence) po at 43 days of gestation Collection at parturition
38. Reproductive failure - Diagnosis? Main complaint: abortion ≠ stages of gestation Fetuses fresh, rigor mortis (firm) Fetuses not fresh, partially mummified Lungs/spleen fetuses (esp. with necrotic foci in liver) No serum sows!!! Immunofluorescence Virus isolation/PCR ADV, CSFV Sera sow Seroconversion SIV or other pathogen causing general disease fever
39. Reproductive failure - Diagnosis? Main complaint: SMEDI Fetuses/piglets at birth Mummies (≠ stages), stillborn and normal piglets 1) Heart/spleen/lungs fetuses (<70 dpins; <17cm) 2) Body fluid/serum fetuses (>70dpins; >17cm) No serum sows!!! 1) Virus isolation/PCR 2) Antibody detection (BE CAREFULL WITH PCV2!!!) PPV, PCV2, PEV, EMCV
40. Reproductive failure - Diagnosis? Main complaint: late abortion/early farrowing Fetuses at late abortion/early farrowing Early stages of mummification (brown), stillborn and normal piglets Placenta/lungs/spleen fetuses Serum stillborn/piglets before colostrum uptake No serum sows!!! Virus isolation(±)/PCR PRRSV
43. Gilts OK PPV √ 40-100 kg 10-20 kg Sows Sows + offspring OK 20-40 kg OK
44. Gilts PPV √ 40-100 kg 10-20 kg Sows ! ! ! Sows + offspring 20-40 kg
45. Gilts Gilts V V V PPV OK √ 40-100 kg 10-20 kg Sows OK OK Sows + offspring 20-40 kg V
46. Gilts PCV2 OK √ 40-100 kg 10-20 kg Sows Sows + offspring OK 20-40 kg OK
47. Gilts PCV2 √ 40-100 kg 10-20 kg Sows ! ! ! Sows + offspring 20-40 kg
48. Gilts Gilts V V PCV2 V OK √ 40-100 kg 10-20 kg Sows OK OK Sows + offspring 20-40 kg V
49. Gilts PRRSV OK √ 40-100 kg 10-20 kg Sows Sows + offspring OK 20-40 kg OK
50. Gilts PRRSV √ 40-100 kg 10-20 kg Sows ! ! ! Sows + offspring 20-40 kg
51. Gilts Gilts V V PRRSV V OK? √ 40-100 kg 10-20 kg Sows OK OK Sows + offspring 20-40 kg V
52. Gilts PRRSV √ 40-100 kg 10-20 kg Sows ! ! ! ! Sows + offspring 20-40 kg
53. Gilts PEV OK √ 40-100 kg 10-20 kg Sows Sows + offspring 20-40 kg
54. Laboratory of Virology & Laboratory of ReproductiveTechnology Faculty of VeterinaryMedicine GhentUniversity, Belgium H. Nauwynck Van Soom G. Labarque R. Sanchez G. Vanroose S. Tanghe B. Mateusen P. Meerts D. Lefebvre D. Saha U. Karniychuk E. Bussalleu J. Beek Virus-embryo/fetus work