1. Michael James Bagley
625 McDonald Lane Escondido, CA 92025 760.715.0997 mjbagley14@gmail.com
OBJECTIVE To obtain a full-timeposition applyingmy biochemistry experience toward research and
production within the scientific community.
EDUCATION Bachelor of Arts, Biochemistry; Minor in Philosophy
University of San Diego – USD, San Diego, CA 92110
Graduation Date: May 2014
AREAS OF EXPERTISE: Protein Purification and BindingAssays Plasmid DNAModification Buffer Preparation and Dilutions
Column Chromatography Dialysis Spectroscopy Monitoringthe Growth of Bacterial Colonies Gel Electrophoresis
Crystallizations PolymeraseChain Reaction HPLC Transformation and Inoculation UsingBacterial Cells Maintenance
of Lab Notebook Organization of Workspace
TECHNICAL SKILLS: Microsoft Excel Column Filtration Systems Autoclave 400/500 MHz Nuclear Magnetic
Resonance (NMR) Machine UV/vis and Mass Spectrometers Electrophoresis Kits Centrifuge Pipetman Restriction
Enzymes Geneious EndNote Protein Data Bank Material Safety Data Sheets PyMol
RELEVANT COURSES: Biochemistry Organic Chemistry Physical Chemistry Analytical Chemistry Cellular Biology
Biophysics Instrumental Analysis Calculus II
ACADEMIC AWARDS/LEADERSHIP: Alumni Research Fellowship, USD, Summer 2012, SURE/PURE Research Grants, USD,
2010 - 2013, First Honors, USD Academics, Fall 2010, Excellence in Research, USD, May 2014, Student Ambassador, USD,
Spring 2011 – Fall 2013, Undergraduate ASBMB member, Fall 2012-Fall 2013
CERTIFICATIONS: Handling and Cleanup of Hazardous Materials Basic Lab Safety Training Shipping and Handling of
Chemicals, Hazardous, and Agricultural Materials
EXPERIENCE
UNIVERISTY OF SAN DIEGO, Biochemical Researcher June 2010 - December 2013
Modified and expressed DNA constructs in bacterial cellsto produce full-formor truncated proteins. Proteins were
then purified via ion exchange,hydroxyapatite,and sizeexclusion chromatography and subjected to bindingassays.
Determined protein abundance and stability via crystallization tests.
Experience culturing microorganisms, such as various E. coli bacteria strains, containing yeast DNA and proteins.
Operated specialized equipment such as UV/vis spectrometers and gel electrophoresis rigs and was able to
troubleshoot basic problems.
Collaborated with faculty to design and test hypotheses as well as discussing results.
Performed data interpretation and initial analysis of results. Translated results into publication material.
Presented project goals, methods, and results at the 2013 Experimental Biology Conference in Boston, MA.
ONTRAC, Package Handler/Sorter, Miramar,CA December 2012 - January 2014
Unloaded packages off trucks safely and sorted them to different areas of the warehouse.
Operated heavy equipment to transportpackages.
Lifted packages excess of 75 lbs.
A DELIGHT OF FRANCE, Server/Team Leader/Barista/Sous chef, Escondido, CA April 2013 - Present
Served customers by taking their orders, running food, and closing their bills.
Performed sous chef duties such as preparing appetizers and entrees as well as resupplying ingredients.
Managed coworkers so that restaurant procedures remain efficient.
Opened and closed, making sure everything was stocked and cleaned up to code.
Managed inventory.
2. PERSONAL STATEMENT: I am an energetic, enthusiastic,determined, and hard-workingindividual. I quickly master new skills
and learn easily. I also happen to be a crafty person; I knit, paint, draw, and design. I enjoy bringing this aspect of my
personality to the workplace to aid me in thinking abstractly when needed and to bring excitement to my field of study. I
work exceptionally well with others; I listen to the adviceand directions of my superiors as well as devote time and attention
to those who requiremy expertise. I guarantee that I am a great addition to any workplaceand will diligently work to the end
of every task I am given. The biological and chemical sciences have always appealed to me for that they consistently provide
new learning experiences through discovery.
