1. What Neurons Do Action Potential Adapted from AP Psychology: 2006-2007 Workshop Materials “Special Focus: The Brain, the Nervous System, and Behavior”, Basic Neuroscience. Thomas, David G.

2. Action Potential A brief electrical charge that travels down a neuron’s axon.

4. Not all synapses involve the excitatory neurotransmitter glutamate. By some estimates, up to 80 percent of the synapses in the brain do not involve excitation at all but serve instead to inhibit neurons. The most common inhibitory neurotransmitter is gamma-amino-butyric acid, or GABA for short. GABA is released by axonal endings in the same way that glutamate is released, and GABA also traverses the synaptic gap and binds to receptors on dendrites. GABA, too, fits into these receptors like a key in a lock and opens neurotransmitter-gated channels. However, instead of opening +Na channels, GABA opens either –Cl channels or +K channels. From our discussion of the resting potential, we know that the net effect of the forces working on +K ions is to push them out of the neuron. The resulting effect is to make the –70 mV potential inside the neuron even more negative, say to –72, –75, or even –80 mV.

5. This process pushes the neuron’s voltage level further away from its threshold of –65 mV. Therefore more excitation is now required to fire an action potential: the greater the outflow of +K ions, the more the inhibition and with it the decreased likelihood that the neuron will fire. The outflow of +K ions creates inhibitory postsynaptic potentials (IPSPs). The inside of the neuron is pushed away from its threshold, that is, it is hyperpolarized.

6. Integration of EPSPs and IPSPs With something like 10 to 100 billion neurons in the brain, it is quite likely that at any given moment, a neuron is receiving information (that is, neurotransmitters) from several other neurons. Some of this information will be excitatory in the form of glutamate and some inhibitory in the form of GABA. If the net voltage—the sum of all of the resultant EPSPs and IPSPs—reaches the –65 mV threshold, the neuron will fire an action potential. But it is important to remember that this threshold voltage cannot occur just anywhere in the neuron. It must occur at the axon hillock. In other words, it is at the axon hillock where the integration of input takes place. It is as if a little man with a calculator resides at the axon hillock, adding EPSPs and subtracting IPSPs. If and when his calculator reaches –65, the little man presses a button that starts a cascade of events that results in the action potential.

7. The Action Potential Of course, there is no little man residing in each neuron. Instead, there are voltage-gated sodium channels, which open when the threshold voltage of –65 mV is achieved at the axon hillock. These voltage-gated sodium channels are located all along the length of the axon, but initially only those at the axon hillock open. A great many positively charged +Na ions rush into the neuron at this location, causing the voltage to go from –65 up to +50 mV. This spike of positive voltage is the action potential.

9. the size of the action potential stays at +50 mV down the whole length of the axon, regardless of how long the axon is. Thus we say that action potentials are nondecremental—unlike EPSPs and IPSPs, which lose their intensity as they move through the dendrites and cell body. Action potentials are nondecremental because they self-perpetuate in the manner shown in Figure 5. This holds true even for axons that branch many times. At the end of each branch, when the action potential arrives, it is still +50 mV. Action potentials are always the same size: if they occur, they are +50 mV, not +20 on some occasions and +80 on others. This is to say that action potentials are all or none.

10. Another subtle point shown in Figure 5 is that, at Time 2, the local environment near the axon hillock has returned to its resting potential as the action potential travels down the axon. Two processes are primarily responsible for returning the axon to –70 mV. The first is that shortly after +Na ions begin to flow into the axon, the depolarization they produce triggers voltage-gated potassium channels. These channels have a somewhat higher threshold than sodium channels, so they open after the action potential is well under way. Consequently, +K ions begin to flow out of the axon. This removal of positively charged ions begins to push the voltage down toward zero and back into the negative range

11. The second process that serves to reset the local environment to –70 mV is that the voltage-gated sodium channels close when the action potential reaches its peak, stopping the flow of positively charged ions into the neuron. The potassium channels remain open after the sodium channels close, and thus the outflow of +K ions continues until the voltage reaches the resting potential, thus “recharging the battery” so that the axon hillock is ready to initiate another action potential. The entire process from the time sodium channels open until potassium channels close is approximately one millisecond (one-thousandth of a second). During this time, another action potential cannot be initiated at the axon hillock, and thus this duration is referred to as the absolute refractory period. This puts a limit on the firing rate of a neuron to about 1,000 times per second.

12. Stop Take a minute to view the embedded “Action Potential” video in Blackboard.