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LABORATORY DIAGNOSIS OF MALARIA
Picture Guide
1 Preparing blood films
1.1 Preparation of a thin and a thick blood film on the same slide
Clean the finger Puncture the ball of the finger by lancet Collect the blood
Make thin film from small drop Make thick film from large drop Number the slide (lead pencil)
1.2 Common faults in preparing blood films:
Poorly positioned blood films Too much blood Too little blood
Greasy slides Edge of the spreader slide chipped

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2 Examining blood films
How blood elements appear in thick and in thin blood films?
3 Examining blood films for malaria Parasites
3.1 Parts of a malaria parasite in a red blood cell
3.2 Effect of pH on parasite morphology
3.3 Stages of the malaria parasite
3.3.1 The trophozoite stage
N = Neutrophil,
E= Eosinophil,
M = Monocyte,
L = Lymphocyte,
P = Platelets

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3.3.2 The schizont stage
3.3.3 The gametocyte stage
4 Species of malaria parasite(in Giemsa-stain)
1. Plasmodium falciparum 2. Plasmodium vivax
Thin film Thick film Thin film Thick film

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3. Plasmodium malariae 4. Plasmodium ovale
Thin film Thick film Thin film Thick film
Collected By: Nofel Saleem Asswiel
Medical Student ,Aden University
Second Year 2012/2013
E-mail: Nofel166@gmail.com
@nasswiel
facebook/nasswiel