This document summarizes the results of an experiment testing the effect of detergent concentration and type on signal intensity and spot morphology when printing oligonucleotides on NEXTERION® Slide E microarray slides. The experiment found that Triton X-100 at concentrations between 0.015-0.04% produced uniform spot intensity, shape, and diameter with low variability in spot sizes. Both detergent concentration and type influenced spot size and signal intensity, with lower concentrations producing less uniform spots and higher concentrations risking irregular spot morphology. The optimal detergent must be determined empirically based on the specific coating, probes, and printing method used.