1. Pooja Patel
3302 Birch Creek Dr.
Kingwood, TX 77339
pj.patel@utexas.edu
(832)964-8744
OBJECTIVE
Ambitious and versatile molecular graduate seeking to further excel in the molecular diagnostic field through laboratory
technician experience.
EDUCATION
The University of Texas at Austin, Austin, TX Aug 2010—May 2014
Bachelor of Science in Biochemistry
The University of Texas at MD Anderson, Houston, TX Aug 2014—Aug 2015
Bachelor of Science in Molecular Genetic Technology
 GPA: 3.91
CERTIFICATION
Molecular Biology (ASCP)CM Aug 2015—Aug 2017
LABORATORY EXPERIENCE
University of Texas at MD Anderson
Molecular Genetic Technology
 Performed DNA extraction, DNA quantitation, bisulfite treatment, variations of polymerase chain reaction, and Sanger
sequencing
 Applied molecular biology to troubleshooting different molecular laboratory techniques
 Optimized polymerase chain reactions using bioinformatics
Molecular Health
Intern
 Prepared a genomic library for Next Generation Sequencing Illumina® HiSeq platform
 Analyzed a variant list generated from a former Illumina® HiSeq run in order to generate a list of effective treatment
options
 Briefed company members on emerging challenges and findings in solid tumor cancers and therapies
University of Texas Medical Branch
Intern
 Comprehended the testing methods behind testing for infectious disease
 Performed and evaluated HCV viral load using real—time PCR
University of Texas at Austin, Zhang Lab
Undergraduate Researcher
 Half-activated and purified wild-type and C20IV mutant of human asparagine-like protein I using nickel column and
HPLC purification systems
 Performed prep analysis for Human Asparagine-like Protein I wild-type/mutant
 Updated current protein purification procedures to maximize protein yield
 Received the Undergraduate Research Fellowship for research on investigating Asparagine—like Protein I mutant
structure to understand the wild—type protein’s function as a replacement therapy for current treatments used in
Acute Lymphocytic Leukemia
LABORATORY SKILLS
PROFICIENT IN:
 DNA Extraction
o Peripheral Blood and Buccal Sample
 Qiagen DNA Extraction Kit, Gentra Puregene Kit
 Quality Control—Spectrophotometry and Nanodrop

2.  Polymerase Chain Reaction Conventional, multiple targets set up in different reactions, Multiplex PCR, Touchdown
PCR, “Hot—Start”, Reverse Transcription PCR
 SDS—PAGE and Agarose Gel Preparation and Run
 Sanger Sequencing (ABI 310 Genetic Analyzer)
 Bioinformatics
o NCBI databases including GenBank, RefSeq, BLAST suite, OMIM, dbSNP, HaploMap, GENE
o Other databases such as UCSC, Ensembl, Protein Data Bank
 PyMOL
 High—Performance Liquid Chromatography
o Ion Exchange
o Size Exclusion
 Protein Expression and Purification
 Crystal Tray Set—Up
EXPERIENCE WITH:
 Formalin—Fixed Paraffin—Embedded Tissue Section DNA Extraction
 RNA Extraction
o Peripheral Blood and Buccal Samples
 Organic extraction using TRIzol reagent
 QC—Nanodrop and native agarose gel electrophoresis
 Real—Time PCR, Methyl—Specific PCR
 Protocol Development
o Primer Design
o Polymerase Chain Reaction Optimization
 Restriction Fragment Length Polymorphism Analysis
 Library preparation for Illumina® HiSeq
FAMILIAR WITH:
 Microsatellite Instability Analysis
 Profiling by Short Tandem Repeat Analysis
 Hybrid Capture
 Microarray
 Fluorescence In Situ Hybridization
 Pyrosequencing
 Next Generation Sequencing
o Ion Torrent™ Personal Genome Machine
o Illumina® Hiseq 2500 Library Preparation
AWARDS/ACHIEVEMENTS/CERTIFICATIONS
The Undergraduate Research Fellowship at the Spring 2014
University of Texas at Austin
Biology Scholars Program at the University of Spring 2012
Texas at Austin
LEADERSHIP EXPERIENCE
The Project
Senior Team Leader
 Coordinated with community counsel and The Project members to improve impoverished Austin communities
 Planned and executed the restoration of a convent in a neglected community
 Corresponded with community leaders in order to obtain the necessary materials and volunteers for the restoration
REFERENCES AVAILABLE UPON REQUEST