Identification of a new way to induce differentiation of dermal fibroblasts into vascular endothelial cells.pdf
1. 1
BY: Santiago Giraldo Rodas.
III semestre. Facultad de medicina de la
Universidad Pontificia Bolivariana
2. Human Dermal
Fibroblasts
Human dermal fibroblasts (HDFs) in the skin are derived from mesenchymal stem cells (MSCs) during embryonic development. HDFs
are abundant in the human body, they contribute to the formation of connective tissue and have multi-directional differentiation
potential.
When the body is damaged, fibroblasts are activated to participate in the production of the extracellular matrix and repair damaged
tissues.
2
INTRODUCTION
3. Vascular
Endothelial Cells
It is the type of flat cell that lines the inside of all blood vessels and is in permanent contact with the blood.
They have antithrombotic functions, selective permeability, modulation of blood flow and vascular resistance, regulation of immune
responses, hormone synthesis, and lipoprotein modification.
3
INTRODUCTION
4. Identification of a new way to induce
differentiation of dermal fibroblasts
into vascular endothelial cells
4
OBJECTIVE
5. Western blot analysis
5
METHODS
Quantitative real-time PCR
It is a technique for identifying specific
proteins in a complex mixture of proteins.
Proteins are separated by electrophoresis and
then incubated with antibodies that react with
the protein of interest.
It is a test to amplify a specific sequence of
genetic material and see how much it was
amplified to see the expression of the gene
They used it to see the level of expression of
the markers and genes of the HDF and VEC
It was used to see the level of proteins of the
HDF and VEC.
6. Immunofluorescence
microscopy
6
METHODS
Flow cytometry
It is a complex technology that allows to
analyze a cell population providing a wide
variety of parameters, which can be
correlated and displayed in different formats.
It is an immunolabeling technique that uses
antibodies chemically linked to a fluorescent
substance to demonstrate the presence of a
certain molecule. The microscope is then
used to analyze images individually or
overlaid.
It was used to see the level of proteins of the
HDF and VEC.
It was used to see the level of VEC´s proteins
and VEC´S gene expression after treatment of
HDF.
9. DISCUSSION
AUTHOR WHAT THEY SAID AGREE / DISAGREE
Li L and Nourse MB (37-38) The VEGF signaling pathway
plays a regulatory role in the
differentiation of endothelial
progenitor cells into VECs
Agree
Kemp SS and Bowers SLK(39-
40)
PDGF signaling pathway also
plays an important role in the
diferentiation of endothelial
progenitor cells into mature
endothelial cells
Agree
Kano MR (41) VEGF and FGF-2 synergistically
activate the endogenous
PDGFB-PDGFRβ signaling
pathway
Agree
10. 10
CONCLUSIONS
Thanks to the different techniques in
molecular biology, great advances can be
made that could allow new medical
treatments in the future, as in this case,
an alternative to repair damaged blood
vessels and prevent ischemic processes.
The understanding of molecular
processes in cells allows scientific
progress to propose new ideas for
medical intervention in different fields,
such as cell therapy in tissue recovery,
cancer prevention, treatments for
chronic diseases, etc.
