Histology Notes

1. CONNECTIVE TUSSUE
DIVERSE GROUP OF CELLS
EMBEDDED IN A TISSUE SPECIFIC
EXTRACELLULAR MATERIAL

2. COMPONENTS OF C.T.
 EXTRACELLULAR MATERIAL
FIBERS
GROUND SUBSTANCE
TISSUE FLUID
 CELLS

3.  MESENCHYMAL ORIGIN
 STRUCTURAL & FUNCTIONAL
DIVERSITY
 CLASSIFIED ON BASIS OF
COMPOSITION & ORGANIZATION OF
COMPONENTS

4. CLASSIFICATION
 EMBRYONIC
MESENCHYMAL
MUCOID
 C.T. PROPER
GENERAL PROPERTIES
SPECIAL PROPERTIES
 SPECIALIZED

5. MESENCHYMAL
 SPINDLE SHAPED CELLS WITH
PROCESSES
 VISCOUS GROUND SUBSTANCE
 FINE COLLAGEN FIBER

7. MUCOID
 SPINDLE SHAPED CELLS WIDELY
SEPARATED
 GELATINOUS GROUND SUBSTANCE
 WISPY COLLAGEN FIBERS
 ADULTS PULP OF TOOTH VITREOUS
CHAMBER OF EYE NUCLEUS
PULPOSUS

8. C.T. PROPER
 GENERAL PROPERTIES
LOOSE
DENSE REGULAR & IRREGULAR
 SPECIAL PROPERTIES
ADIPOSE
RETICULAR
PIGMENTED

9. LOOSE C.T.
 CELLULAR WITH FEW FIBER
 GROUND SUBSTANCE
 SUPERFICIAL FACIA

11. CELLS OF CONNECTIVE TISSUE
 FIBROBLAST
 MACROPHAGES
 PLASMA CELLS
 MAST CELLS
 ADIPOSE CELLS
 WANDERING CELLS

12. FIBROBLAST
 PRINCIPAL CELLS
 ACTIVE & INACTIVE (FIBROBLAST OR
FIBROCYTE)
 ABUNDUNT CYTOPLASM, PALE STAINING
NUCLEUS WITH PROMINENT NUCLEOLUS
 LARGE FLATTENED OVOID CELLS, BRANCHING
PROCESSES FLATTENED NUCLEI
 FIBERS & GROUND SUBSTANCE
( WOUND REPAIR)

15. MACROPHAGES
 PHAGOCYTIC CELLS DERIVED FROM
MONOCYTES
 IRREGULAR, SHORT BLUNT PROCESSES &
ECENTRIC KIDNEY SHAPED NUCLEUS
 LOCATIONS
 KUPFFER, MICROGLIAL, LANGERHAN,OSTEOCLAST
 SUPRAVITAL STAINING
 FUNCTIONS
PHAGOCYTOSIS,ANTIGEN
PRESENTING, ENZYME PRODUCTION
(COLLAGENASE)
CYTOKINES (TUMOUR CELL KILLING
CAPACITY)

16. PLASMA CELLS
 B LYMPHOCYTES
 OVOID IREGULAR,BASOPHILIC(r
ER), CART WHEEL NUCLEUS &
UNSTAINED AREA NEAR THE
NUCLEUS
 MAJOR SOURCE OF CIRCULATING
ANTIBODIES
 MUCOUS MEMBRANE, CHRONIC
INFLAMMATION & RETICULAR C.T.

18. MAST CELLS
 LARGE CELLS WITH SMALL OVOID
NUCLEUS & METACHROMATIC
GRANULES
 B.V. MUCOUS MEMBRANE & SKIN
 ABSENT IN C.N.S.
 HEPARIN & HISTAMINE

20. FAT CELLS
 SIGNET RING APPEARANCE
 SPECIALIZED TO STORE NEUTRAL
FAT
 UNILOCULAR MULTILOCULAR
 OSMIC ACID & SUDAN III

21. ADIPOSE CELLS

22. WANDERING CELLS
WHITE BLOOD CELLS

23. FIBER
 COLLAGEN COLLAGEN
 RETICULAR COLLAGEN
 ELASTIC ELASTIN

24. COLLAGEN FIBER (L/M)
 PROTEIN COLLAGEN MOST ABUNDUNT
 FLEXIBLE WITH HIGH TENSILE STRENGTH
 WAVY BUNDLES VARIABLE LENGTH &
DIAMETER
 ACIDIC DYES
EOSIN PINK
ANILINE BLUE BLUE
MASSON TRICHROME GREEN

25. FIBRILS (E/M)
VARIABLE
68 nm CROSSBANDING
TROPOCOLLAGEN
SPECIFIC ARRANGEMENT
COVALENT BONDS

27. TROPOCOLLAGEN
 TRIPPLE HELIX
 HYDROXYPROLINE(LYSINE), GLYCINE
PROLINE
 300-6000 AA
 27 TYPES OF CHAINS
 19 DIFFERENT COLLAGEN

29. SYNTHESIS
 INTRACELLULAR
RIBOSOMES POLYPEPTIDE CHAIN
R.E.R POST TRANSLATIOAL
MODIFICATION
EXOCYTOSIS PROCOLLAGEN
(VIT. C SCURVY)
 EXTRACELLULAR
TROPOCOLLAGEN PEPTIDASE
ALIGNMENT FIBRILS

30. COLLAGEN TYPES
 TYPE I LOOSE & DENSE C.T.
 TYPE II HYALINE & ELASTIC
CARTILAGE
 TYPE III C.T. OF ORGANS
RETICULAR FIBER
 TYPE IV BASAL LAMINA

31. RETICULAR FIBERS
 TYPE III
 FIBROBLAST
 THINNER, THREAD LIKE,68nm
 LOOSELY PACKED, INTERFIBRILLAR
BRIDGES
 SUPPORTING FRAME
WORK, SURROUNDS ADIPOSE &
MUSCLE CELL, B.V. & NERVES
 ARGYROPHILLIC, PAS

33. ELASTIC FIBER
 THINNER BRANCHING
 FIBROBLAST, SMOOTH MUSCLE
 ELASTIN (PROLINE GLYCINE)
 FIBRILLIN GLYCOPROTEIN MICROFIBRILS
 DESMOSINE & ISODESMOSINE
 ELASTIC PROPERTY IS DUE TO RANDOM
COILING OF ELASTIN MOLECULES
 VERTEBRAL LIGAMENTS, LARYNX &
ELASTIC ARERIES
 ORCEIN RESORCEIN FUCHSIN
 MARFAN SYNDROME

35. GROUND SUBSTANCE
COLOURLESS TRANSPARENT
VISCOUS GEL PRESENT B/W CELLS &
FIBERS OF CONNECTIVE TISSUE