Lab'InSight Fermentation dans l'industrie alimentaire, du 29.10.2015, organisé par le Réseau LIEU à Gembloux Agro-Biotech Présentation du prof. Anne-Lise Hantson (UMONS) Présentation

1. RESEARCH
INSTITUTE
FOR
BIOSCIENCES
UMONS
Prof.
Anne-­‐Lise
Hantson
Associate
Professor
«
High
content
FOS
produc3on
and
purifica3on
:
an
integrated
bioprocess
»
FERMENTATION
IN
THE
FOOD
INDUSTRY
:
Managing
Living
Organisms
to
op0mize
your
Produc0on

2. FIELDS
OF
EXPERTISE
 Protein
pre-­‐frac0ona0on,
characteriza0on,
expression
and
purifica0on
 High
throughput
proteomics
(proteins
iden0fica0on
and
quan0fica0on)
 Biomarkers
discovery,
valida0on
and
assay
 Bioprocess
experimental
studies
for
specific
industrial
applica0ons
(Agro-­‐Food,
Pharmaceu0cal,
White
biotechnology,
Environnemental
biotechnology)
 SMB
separa0on
 Modeling
of
biological
systems
and
bioprocesses
 Op0miza0on
and
control
of
bioprocesses

3. RECENT
APPLIED
RESEARCH
PROJECT
Tradi0onal
process
+
High
content
FOS
producHon/
purificaHon
PDR
4
ans
(UMONS-­‐ULB-­‐U
Minho
(Pt))

4. RECENT
APPLIED
RESEARCH
PROJECT
Use
of
2
specific
micro-­‐organisms
in
one
or
two-­‐stage
fermenta0ons
Fermenta0on

5. RECENT
APPLIED
RESEARCH
PROJECT
Broth
=
mixture
of
salts
and
monosaccharide
and
FOS
Ion
exchange
chromatography

6. RECENT
APPLIED
RESEARCH
PROJECT

7. RECENT
APPLIED
RESEARCH
PROJECT
Batch-­‐to-­‐batch
opHmizaHon
of
a
press
filter
First
entreprise
(FPMs
-­‐
Meura)

8. SERVICE
PROPOSAL
TRAINING,
AUDIT/ANALYSIS,
RESEARCH…
Training
:
-­‐ Protein
Mass
spectrometry
training
and
courses
-­‐ Protein
purifica0on
and
characteriza0on
training
and
courses
Researches
:
-­‐ Process
study
and
op0miza0on
Feed
Medium
(Sin)
Fin
X, S, P, O, C
0
1
2
3
4
5
6
7
-­‐0, 2 0 0, 2 0, 4 0, 6 0, 8 1 1, 2 1, 4 1, 6
a c e ta te
( g / l )

9. SERVICE
PROPOSAL
-­‐
Process
Analy0cal
Technology

10. SERVICE
PROPOSAL
-­‐
Bioprocess
monitoring
and
control
Animal
cell
culture
laboratory
:
global
design

11. SERVICE
PROPOSAL
P1
S1
S2
P2
P3
12
3221
211
5
2
1
...
qPjS
iPhPgSeS
cPbPaS
⎯→⎯
+⎯→⎯+
+⎯→⎯
ϕ
ϕ
ϕ
Simplified
metabolic
path
-­‐
Bioprocess
modeling

12. SERVICE
PROPOSAL
Modèle
dynamique
Bioprocédé
Données
Algorithme
d'idenHficaHon
systémaHque
-­‐
Integrated
Bioprocess
Study

13. SERVICE
PROPOSAL
-­‐ Purifica0on
by
SMB
and
other
chromatographic
technics
-­‐ Metabolomic
and
Proteomic
study
of
micro-­‐organism
‘s
metabolism
for
knowledge-­‐based
process
op0misa0on

14. SERVICE
PROPOSAL
-­‐ MS-­‐based
(MRM)
protein
assay
in
complex
matrices
(new
bioprofiling
plaUorm
UMONS-­‐ULB-­‐Ulg.
Feder
2014-­‐2020)
Amount
loaded:
0
50
100
150
picomoles

15. PROF.
ANNE-­‐LISE
HANTSON
Associate
Professor
biosciences@umons.ac.be
+32
65
37
33
12
/
+32
65
37
44
19
RESEARCH
INSTITUTE
FOR
BIOSCIENCES
Place
du
Parc
21,
7000
MONS
www.umons.ac.be/biosciences
UMONS
@