Culture Collection Center National and International

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Aysha Riza & Rinu Rolly
St Mary's College Thrissur

• Culture collection are centers
that provide authentic
examples of organism that can
be grown or maintained in the
laboratory ; they normally
have a public service role and
provide associated information
and services
• Prof. Frantisek Karl was the
first one to collect the culture .
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https://en.wikipedia.org/wiki/Karel_Franti%C5%A1ek_Koch
Karl Frantisek Koch
Born : 29 June 1890
Died : 24 January 1981
Nationality : Czech

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• Culture Collections aims at acquisition , preservation,
authentication , production preservation, cataloguing and
distribution of standard reference microorganism , cell
lines and other materials for research and repositories of
type strains.

• According to World directory of culture collections (Sixth Version
2014):
* Culture collection : 802
* Countries and region : 78
*Microorganism :3272863
* Bacteria :1429816
* Fungi : 872094
* Virus :39491
* Cell line : 33020
Aysha Riza & Rinu Rolly St Mary's College
Thrissur
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• Collection and maintenance of important and useful organism
• Maintain culture in viable and contaminant free state.
• To make available at nominal cost to researcher ,workers,
teachers ,students or industries .
• Preparation of their information catalogue .
• Comparison of microorganisms
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• Initiative of government
department of biotechnology.
• Housed at National Center of Cell
Science (NCCS), Pune
• Depository of microorganism (for
isolates generated from microbial
prospecting and screening
program initiated by DBT)
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www.nccs.res.in
Microbial collection Center, Pune

• Status of collection – governmental
• Sponsor – Department of Biotechnology ,Ministry of Science
and Technology, Government of India
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Services :
Storage (bacteria, fungi)
Distribution
Identification

Trainings :
 Culture and preservation methods
 Identification
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NUMBER OF STRAINS IN MCC
Type Holding strains (Total
number)
Bacteria 149314
Fungi 15338

• Established in 1986 .
• MTCC roots at Institute of
Microbial Technology (IMTECH),
Chandigarh .
• Sponsor – Department of
biotechnology and Government
of India and Council of Scientific
and Industrial Research .
• Status – Semi governmental
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https://www.imtech.res.in/focus-area/microbial-type-culture-
collection
Microbial Type Culture collection and Gene
bank , Chandigarh

Main objectives
Act as depository
To supply authentic microbial cultures
To provide related services to the scientist working in
research institutions universities and industries .
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Services :
 Distribution (bacteria ,fungi ,plasmids)
 Identification
 Patent deposits
 Storage services
Training :
 Genetic engineering
 Identification
 bacterial genetics
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NUMBER OF STRAINS IN MTCC
Type Holding Strains (Total number)
Algae 1124
Bacteria 1245
Fungi 575
Yeasts 85
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• Established in 1951 .
• One of the oldest biological resource center .
• Status – Governmental
• Housed at National Chemical Laboratory (CSIR) New Delhi
Services:
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 Patent deposits (Bacteria ,yeast , fungi)
 Storage services (microbial strain)
 Distribution (bacteria ,yeast ,fungi ,algae)

Trainings :
 Management of culture collection
 Culture and preservation method
 Genetic engineering
 Systematics.
 Identification
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NUMBER OF STRAINS IN NCIM
Type Holding Strains ( Total number)
Algae 20
Bacteria 1400
Fungi 950
Yeasts 600

• Established in 1962.
• Status of the collection – ICAR.
• Housed at National Dairy
Research Institute ,Karnal
Haryana .
• Sponsor – N.D.R.I, Karnal and
ICAR , New Delhi.
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https://www.showincity.com/venues/v-18667/national-dairy-research-
institute
National Culture of Dairy Collection , Harayana

Services :
 Distribution (bacteria, fungi, yeast, dairy starters ).
Training :
 Culture and preservation method
 Quality control
 Use of computers in culture collections
 Preservation , propagation and quality control of Dairy starter
cultures
 Preparation of fermented milks
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NUMBER OF STRAINS IN NCDC
Type Holding strains (Total number)
Bacteria 400
Fungi 15
Yeasts 20
Dairy starter cultures 25
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• Established in 2008
• Initiative by Department of Science and Technology at Agharkar Research
Institute, Maharashtra.
• NFCCI aims at conserving fungi as resource pool for future research and
related projects.
• Housed at MACS’ Agharkar Research Institute , Maharashtra
• Status – Governmental ,National Facility
• Sponsor – Department of Science and Technology ,Government of India
and MACS’ Agharkar Research Institute , Pune , India .
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Services :
 Storage services (micro and macro fungi)
 Distribution
 Identification
NUMBER OF STRAINS IN NFCCI
Fungi 3050
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Training:
 Management of a culture collection
 Quality control
 Use of computers in culture collection
 Systematics
 Identification
 Molecular systematics
 Fermentation and isolation of secondary products
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• Largest general culture collection in
the world.
• Serves as centre for acquisition ,
preservation, authentication and
distribution of microbial specimens
standard reference microorganism ,
cell lines and other materials for
research and development .
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American Type Culture Collection ,USA
https://www.atcc.org/~/media/PDFs/webinars/Presentations/2017/M
ycoplasma%20Detection%20Webinar.ashx

• Status – Private non profit organization.
• Designated as first International Depository Authority (IDA)
(International Budapest Treaty for biotechnology patent
,1981)
Programs:
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 Collection , Research and service program .
 Professional Service program .
 Information Service program

Services:
Patent deposits .
Storage services (safe deposit).
Identification (authentication services).
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Training:
 Management of culture collection
 Quality control and Genetic engineering
 Karyotyping.
 Use of computer in culture collections .
 Cytogenetics.
 Diagnostic virology
 Fermentation
 Hybridomas and monoclonal antibodies
 Medicinal mycology
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NUMBER OF STRAINS IN ATCC
Type Holding strains
(Total number)
Bacteriology:
Bacteria & bacteriophage
18000
Cell biology:
Cell lines & hybridomas
4300
Molecular biology:
Recombinant DNA Material
0
Mycology &botany :
Filamentous fungi & yeasts
46000
Plant tissue 79
Seeds 1000
Protists (including algae) 2000
Virology: Animal virus, chlamydiae , rickettsiae
& antisera
2200
Plant viruses & antisera 1500
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• Housed and maintained by RIKEN Bio Resource and Research
Center at Tsukuba ,Ibaraki ,Japan
• Status – semi-governmental
• Sponsor – The Japanese government (Ministry of education ,
culture, sports , science and technology )
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• This division collects , preserves and distributes cultured
microbial strain as one of the leading culture center in the
world
• Strains held at JCM are limited to those classified as Risk 1 and
Risk 2
• ISO Certified for its quality management system
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Services :
 Distribution
Training :
 Systematics.
 Identification
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NUMBER OF STRAINS IN JCM
Bacteria 18822
Yeasts 4208
Archaea 838
Microalgae 26
Filamentous fungi 3904
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• Rooted and maintained by Masaryk University
• Status of collection – university
Catalogue of cultures include :
 Bacteria and Archea.
 Filamentous fungi and yeast.
 Bacteriophages.
 Fungi ( deuteromycetes, ascomycetes, mucorales)
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Services :
 Patent deposits (bacteria , fungi , yeasts)
 Storage service (bacteria , fungi)
 Identification (bacteria [aerobic bacteria])
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Training :
• Management of a culture collection
• Culture and preservation methods
• Identification

NUMBER OF STRAINS IN CCM
Type Holding strains(Total number)
Bacteria 2600
Fungi 750
Yeasts 22
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• Serves to “ provide a trustworthy source of authentic bacteria for use in
scientific studies”.
• NCTC is one of the four culture collections of public health England
• Status – governmental
• Sponsor – department of health
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• It is longest established collection of its type anywhere in the world
• It also serves as United Nations Educational , Scientific and Cultural
Organization(UNESCO) Microbial Resource Center (MIRCEN)
• NCTC holds nearly 6000strains most of which have caused infections in
humans or animals and most of them are medical , scientific and
veterinary importance world wide
• It also serves as an International Depository Authority (IDA) patent
depository for bacterial cultures.
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Strains grouped by specific characteristics
 Antibacterial susceptibility control and resistance reference strains
 Control strains for the microbiological testing of water samples for
Pseudomonas aeruginosa.
 Clostridium difficile strains – Ribotype and toxin status
 NCTC reference strains for standards for microbiology investigations (UK-
SMI)
 World Data Center for Microorganism (WDCM) reference strains
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Services
 Patent deposits (bacteria)
 Storage service (bacteria)
 Distribution (bacteria)
 Identification (bacteria)
NUMBER OF STRAINS IN NCTC
Bacteria 5000
Plasmids 600
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• Established in 1979 as central
culture collection network of
various collection.
• Rooted at institution of
microbiology , China academy of
science
• Status – governmental
• Sponsor – Chinese academy of
sciences
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http://www.cgmcc.net/
China General Microbial Culture Collection
center ,China

• ISO certified
• Focus – to preserve , supply and maintain living microbial resources .
Contribute to scientific community by providing high quality microbial
source to research fields.
• Maintain approximately 40000 by the end of 2013 including
bacteria,archea, fungi
• Strains held at CGMCC classified to risk 1 0r 2 group
• Information of strain available at online catalogue database of CGMCC
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• 8700 strain for patent purpose
• To improve and expand collection continously CGMCC exploit new
resources from various environment , describing microbial taxa,
accept useful microbes researcher and applied sectors
• All strains are subjected to extensive quality control
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Services :
• Patent deposit (Archea ,Bacteria, Yeasts, Fungi, Algae, Viruses, plasmids,
cell lines, Plant seed)
• Storage services (Archea ,Bacteria, Yeasts, Fungi)
• Distribution (Archea ,Bacteria, Yeasts, Fungi , cell lines)
• Identification (Archea ,Bacteria, Yeasts, Fungi )
NUMBER OF STRAINS IN CGMCC
Type Holding strains
Bacteria 19820
Fungi 16750
Yeast 5360
Cultures for patent purpose 11976
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• Avinash Sharma and Yogesh Shouche. (2014). Microbial Culture Collection (MCC) and
International Depositary Authority (IDA) at National Centre for Cell Science , Pune . Indian
Journal of Microbiology .129-133.
• https://mtccindia.res.in/
• https://www.ncl-india.org/files/ncim/Default.aspx
• http://www.ndri.res.in/ncdc/Members/NCDC_Catalogue_2016.pdf
• http://nfcci.aripune.org/
• http://nfcci.aripune.org/about_us.php
• http://gcm.wfcc.info/
• http://www.wfcc.info/index.php/wdcmdb/
• http://www.wfcc.info/ccinfo/collection/by_country/u/
• http://www.wfcc.info/ccinfo/collection/by_country/j/
• http://www.wfcc.info/ccinfo/collection/by_country/c/
• http://www.wfcc.info/ccinfo/collection/by_country/i/
• http://www.nccs.res.in/mcc/index.html
• http://www.imtech.ernet.in/mtcc/
• http://www.ndri.res.in/
• http://http.atcc.org/
• http://jcm.brc.riken.jp/
• http://www.szu.cz/cnctc/
• www.phe-culturecollections.org.uk
• http://www.cgmcc.net
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Culture Collection Center National and International

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