1. DR. RYAN M. CHANYI, PHD, HON. BMSC.
London, ON, Canada · Cell: 519.619.5202 · rchanyi@gmail.com
1
EDUCATION
Lawson Health Research Institute;
Canadian Centre for Human Microbiome and Probiotic Research;
Integra Medical Inc.
Postdoctoral Fellow (Joint: Industry & Academia)
Supervisor: Drs. Peter Cadieux, Jeremy Burton & Ms. Natalya Iudina
University of Western Ontario, London, Ontario
Doctor of Philosophy
Department of Microbiology and Immunology
Supervisor: Dr. Susan Koval
University of Western Ontario, London, Ontario
Master of Science (transfer to PhD)
Department of Microbiology and Immunology
Supervisor: Dr. Susan Koval
University of Western Ontario, London, Ontario
Bachelor of Medical Science Honour Specialization in Microbiology & Immunology
(With Distinction)
Thesis Supervisor: Dr. Susan Koval
Waterford District High School
Ontario Secondary School Diploma
Present
2014
2011
2009
2004
RESEARCH EXPERIENCE
Fanshawe College, London, Ontario
Part-time Professor
Part-time Professor in the School of Health Sciences at Fanshawe College. Teach
introductory biology to +200 students in the pre-health program. The course is designed to
give a broad introduction into biology and its concepts to students who wish to work in the
health care field but have not chosen a specialty
Lawson Health Research Institute, London, Ontario;
Canadian Centre for Human Microbiome and Probiotic Research;
Integra Medical Inc.
Postdoctoral Fellow
i) Role of testosterone production by the gut microbiome in prostate cancer disease
severity, progression and treatment
Detected testosterone being produced from bacteria grown from faecal samples of patients
with prostate cancer. As androgen deprivation therapy is used as a treatment, testosterone
produced from the gut microbiome may play a pivotal role in resistance to conventional
therapy.
ii) Modulation of the gut microbiome to decrease recurrent kidney stone formation
Established a single stage chemostat model of the distal colon to observe how dietary changes
affect oxalate utilization by the gut microbiome. Also established a Drosophila melanogaster
model of oxalate kidney stone formation in the lab.
iii) Probiotic ethanolamine utilization may decrease Salmonella pathogenicity and
infectivity
Ethanolamine is thought to provide a competitive advantage for pathogens to colonize the
intestinal tract. We have found the first probiotic bacterium capable of utilizing ethanolamine.
Co-administration with Salmonella enterica serovar Typhimurium shows promise in
downregulating virulence-associated genes as well as inhibiting pathogen growth.
Sept. 2016
2014 – Present

2. DR. RYAN M. CHANYI, PHD, HON. BMSC.
London, ON, Canada · Cell: 519.619.5202 · rchanyi@gmail.com
2
iv) Surgical optimization of inflatable penile prosthesis implantation
Many inflatable penile prostheses require the device to be submerged in antibiotics prior to
implantation. This process has not been examined to determine if temperature, time
submerged or the antibiotic used plays a role in the ability for the antibiotic to adhere to the
surface, and thus, prevent infection. We are currently screening different devices using a wide
panel of clinically relevant antibiotics to inhibit four major uropathogens; Escherichia coli,
Proteus mirabilis, Enterococcus faecalis and Staphylococcus aureus.
v) Treatment of oral candidiasis using probiotics
Oral candidiasis is a common infection that occurs in immunocompromised patients, namely
infants, elderly and HIV-infected individuals. We examined the mechanism by which our
probiotic treatment inhibited this infection. Our study found that, not only did the probiotic
kill Candida albicans and inhibit biofilm formation, it altered the gene-regulation of necessary
colonization factors preventing pathogen binding and subsequent infection. (James et. al.
2016)
University of Western Ontario, London, Ontario
Doctoral Thesis
Cell biology of the entry of Bdellovibrio and like organisms
i) Type IV pili are not required for predator invasion into the prey cell. (Chanyi and
Koval, 2014)
ii) Bdellovibrio bacteriovorus can be used to kill the cystic fibrosis pathogen,
Stenotrophomonas maltophilia. (Chanyi et al. 2016)
University of Western Ontario, London, Ontario
Summer Research Student
Supervisor: Dr. Susan Koval
University of Western Ontario, London, Ontario
Fourth Year Research Project
Predator-prey interactions of Bdellovibrio and like organisms
Investigating the differencing between the epibiotic and periplasmic life cycles used by the
obligate bacterial predators, Bdellovibrio exovorus and B. bacteriovorus, respectively.
2009-2014
2009
2007-2008
PRIMARY PUBLICATIONS
Chanyi, R.M., Koval, S.F. and Brooke, J.S. (2016) Stenotrophomonas maltophilia biofilm reduction by
Bdellovibrio exovorus. Environ Microbiol Rep [E-Pub ahead of Print] doi: 10.1111/1758-2229.12384
James, K.M., MacDonald, K., Chanyi, R.M., Cadieux, P. and Burton, J.P. (2016) Inhibition of C. albicans biofilm
formation and modulation of gene expression by probiotic cells and supernatant. J Med Microbiol [E-Pub ahead
of Print] doi: 10.1099/jmm.0.000226.
Chanyi, R.M. and Koval, S.F. (2014) Role of type IV pili in predation by Bdellovibrio bacteriovorus. PLoS One
9(11): e113404. doi: 10.1371/journal.pone.0113404.
Pasternak, Z., Njagi, M., Shani, Y., Chanyi, R., Rotem, O., Lurie-Weinberger, M.N., Koval, S., Pietrokovski, S.,
Gophna, U. and Jurkevitch, E. (2014) In and out: an analysis of epibiotic vs periplasmic bacterial predators. ISME
J 8: 625-635. doi: 10.1038/ismej.2013.164.
Chanyi, R.M., Ward, C., Pechey, A. and Koval, S.F. (2013) To invade or not to invade: two approaches to a
prokaryotic predatory life cycle. Can J Microbiol 59(4): 273–279. doi: 10.1139/cjm-2013-0041.
BOOK CHAPTERS

3. DR. RYAN M. CHANYI, PHD, HON. BMSC.
London, ON, Canada · Cell: 519.619.5202 · rchanyi@gmail.com
3
Burton, J.P., Chanyi, R.M. and Schultz, M. (2016) Bacillus. In The Microbiota in Gastrointestinal
Pathophysiology: Implications for Human Health, Prebiotics, Probiotics, and Dysbiosis. Edited by M. Floch, Y.
Ringel and W.A. Walker. Elsevier. [In Press]
Burton, J.P., Chanyi, R.M. and Schultz, M. (2016) Streptococcus thermophilus. In The Microbiota in
Gastrointestinal Pathophysiology: Implications for Human Health, Prebiotics, Probiotics, and Dysbiosis. Edited
by M. Floch, Y. Ringel and W.A. Walker. Elsevier. [In Press]
Chanyi, R.M., Burton, J.P. and Cadieux, P.A. (2015) Pathogenic mechanisms of uropathogens. In The role of
bacteria in urology. Edited by D. Lange and B. Chew. Springer International Publishing Switzerland. pp. 21-32.
Contributed two electron micrographs to:
Madigan et al. 2012. Brock Biology of Microorganisms. 13th
Ed. San Francisco, USA. Pearson Prentice Hall.
p500.
ORAL PRESENTATIONS
Chanyi, R.M., Brinkac, L. and Burton, J.P. (2016) Ethanolamine utilization: Probiotics fight back. Talks on
Fridays, Lawson Health Research Institute, London, ON, Canada.
PUBLISHED ABSTRACTS (*PRESENTING AUTHOR)
Venema, R.*, Brinkac, L., Chanyi, R.M., Razvi, H., Lange, D. and Burton, J.P. (2016) Genetic analysis of human
and animal isolates of Oxalobacter formigenes, an organism of importance to nephrolithiasis. American
Urological Association. Annual Conference. San Diego, USA.
Harvey, B.G.R.*, Chanyi, R.M. and Burton, J.P. (2016) Modulation of adhesion of uropathogenic Escherichia
coli to the uroepithelium using oligosaccharides. London Health Research Day, Western University, London, ON,
Canada.
Chanyi, R.M.*, Brinkac, L. and Burton, J.P. (2015) Ethanolamine utilization by beneficial microorganisms.
Infection and Immunity Research Forum, Western University, London, ON, Canada.
Chanyi, R.M.*, Khursigara, C.M. and Koval, S.F. (2014) Cryoelectron microscopic evaluation of two life cycles
of the bacterial predator Bdellovibrio. Annual Conference. International Union of Microbiological Societies,
Montreal, QC, Canada.
Chanyi, R.M.* and Koval, S.F. (2013) Twitching motility is essential for predation on a biofilm by Bdellovibrio
bacteriovorus. Annual Conference. Canadian Society of Microbiologists, Carleton University, Ottawa, ON,
Canada.
Chanyi, R.M.*, Dresser, A., Dharamshi, J. and Koval, S.F. (2012) Identification of new Bdellovibrio and like
organisms. ASM Conference. Montreal, QC, Canada.
Chanyi, R.M.* and Koval, S.F. (2011) Role of pilT1 and pilT2 in predation by Bdellovibrio bacteriovorus.
Annual Conference. University of Western Ontario, London, ON, Canada.
Chanyi, R.M.* and Koval, S.F. (2011) Characterization of pilT1 and pilT2 in the life cycle of Bdellovibrio
bacteriovorus. Annual Conference. Canadian Society of Microbiologists, Memorial University, St. John’s, NL,
Canada.
Chanyi, R.M.*, Mahmoud, K.K., and Koval, S.F. (2010) Invasion strategies: role of type IV pili in attachment
and invasion of E. coli by Bdellovibrio bacteriovorus. Annual Conference. Canadian Society of Microbiologists,
McMaster University, Hamilton, ON, Canada.
SUPERVISORY EXPERIENCE
Supervision of Visiting Scientists
2015 and 2016
Dr. Fan Li – Role of the microbiome in castrate-resistant prostate cancer
Dr. Thamara Dayarathna – Exposure of Oxalate decarboxylase to Drosophila melanogaster to prevent and
treat oxalate kidney stones

4. DR. RYAN M. CHANYI, PHD, HON. BMSC.
London, ON, Canada · Cell: 519.619.5202 · rchanyi@gmail.com
4
Supervision of Industry Partners
2016
Zachary Klassen, Integra Medical – Evaluation of novel probiotic strains
Jamie Louwagie – Isolation of canine oral probiotic candidates
Jesse Milley – Isolation of human oral probiotic candidates
Julia Jezic – Evaluation of probiotic isolates for novel indications
Kaylen O’Rourke – Development of educational materials
2015
Jesse Milley, Integra Medical – Isolation of novel probiotic candidate strains from oral surfaces
Supervision of Microbiology and Immunology Fourth Year Honor Thesis Students
Richard Berish (2015-2016).
Zach Findlay (2013-2014)
Diane Szmiett (2012-2013)
Jennah Dharamshi (2011-2012)
Amy Dresser (2010-2011)
Supervision of Medical Students
2015 and 2016
Omar Gilani – Role of bacteria transferred during kidney transplantation
Brandon Harvey – Examination of milk oligosaccharides as prebiotics for Lactobacillus
Supervision of Volunteer Students
2016
Everett Leung (2nd
year Western University – Microbiology and Immunology)
Hannah Wilcox (3rd
year Western University – Microbiology and Immunology)
Karlee Searle (3rd
year Western University – Microbiology and Immunology)
Madelaine Bink (3rd
year Western University – Microbiology and Immunology)
Timothy Chan (2nd
year Western University – Microbiology and Immunology)
2015
Richard Berish (3rd
year Western University – Microbiology and Immunology)
Lana Estafanos (4th
year Western University – Kinesiology)
2011
Sarah Walker (2nd
year Western University – Biology)
Emma Farago (2nd
year Western University – Microbiology and Immunology)
2010
Ian Lobb (3rd
year Western University – Microbiology and Immunology)
Jodie Baer (2nd
year Western University – Biology)
Graduate Teaching Assistant
MICRO2100A - Biology of Prokaryotes. From 2010 to 2013. 40 students total.
AWARDS
 Western Graduate Research Scholarship (WGRS): Awarded 2009 to 2013
 2013 Terry Beveridge Poster Award at the Canadian Society of Microbiologists Annual Conference: June 2013,
Ottawa, ON, Canada
 Microbiology and Immunology Travel Award: 2011, 2012, 2013 and 2014
 Graduate Thesis Research Award Fund: 2013
 American Society for Microbiology (ASM) Travel Award
 2011 Canadian College of Microbiologists Poster Award at the Canadian Society of Microbiologists Annual
Conference: June 2011, St. John’s, NL, Canada
 PhD candidacy exam- Passed with distinction: March 2011

5. DR. RYAN M. CHANYI, PHD, HON. BMSC.
London, ON, Canada · Cell: 519.619.5202 · rchanyi@gmail.com
5
 Graduated BMSc with distinction: June 2009
COMMUNITY INVOLVEMENT/ VOLUNTEERING
Judge at 2016 London Health Research Day
Western University, London, ON, Canada
Canadian Society of Microbiologists Student Representative
For The University of Western Ontario; 2010-2014
Judging Coordinator for the Infection and Immunity Research Forum Annual Conference
At The University of Western Ontario, London, ON, Canada; Position held for the 2012 and 2013 conferences
Judge at the London District Science and Technology Fair
Volunteered in 2009, 2012 and 2013
Volunteer for the 2011 Infection and Immunity Research Forum
At The University of Western Ontario, London, ON, Canada