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Cell culture study
1. Cell Culture studiesCell Culture studies
-We have previously shown SPIO particles are taken up
avidly by macrophages. Our cell culture studies showed
a transient elevation in free radical of macrophages with
a rapid return to the baseline.
-Next we tested a new SPIO, (lipid coated) versus the
old (dextran coated).
2. Cell Culture StudiesCell Culture Studies
We have Comparison of the 3 different SPIOs
in the cell culture, and their effect on
macrophage biology,.
Preliminary results indicated that there is a
significant difference between the level of NO
production from macrophages, when they
engulf dextran coated SPIO versus lipid coated
(Feridex is the commercially available SPIO)
3. Induction of NO inInduction of NO in
Macrophages by DifferentMacrophages by Different
forms of SPIOforms of SPIO
Induction of Nitirc Oxide by SPIO and LPIO-particles in
Macrophages -24 h
CNS-1 2d CNS-1 df CNS 5M 1bCNS 5M 2bCNS 5 ML 2cCNS 5 ML 1-6Feridex Control
0.0
0.1
0.2
0.3
CNS-12d
CNS-1df
CNS 5M 1b
CNS 5M 2b
CNS 5 ML 2c
CNS 5 ML 1-6
Feridex
Control
SPIO or LPIO particles
OD550(Nitritelevel)
SPIO
LPIO