Slide shows of uptake of spio by macrophages and monocytes10

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Society for Heart Attack Prevention and Eradication

SHAPE Society

Salud y medicina

Uptakes of SPIO by Murine
Macrophages and Monocytes
Daniel Chan
University of Colorado Health Sciences Center
12-19-2001

Synthesis of SPIO
• 1. SPIO coated with chondroitin sulfate
• 2 SPIO coated with standard dextran (6K)
• 3 SPIO coated with Amino-silane
• 4 SPIO coated with lipids
• 5 Lipid encapsulated SPIO coated with
amino-silane

Uptakes of SPIOs by murine peritoneal
macrophages and monocytes
• Normal mice were treated with mineral oil
by ip injection.
• 24 hours later, equal amount of various
SPIOs were injected intraperitoneally.
• 24 hours later, peritoneal macrophages and
monocytes were isolated.
• Uptakes of various SPIOs were examined
by light microscopy.

Uptake by Mouse Macrophages
SPIO coated with chondroitin sulfate

Uptake by Mouse Macrophages
SPIO coated with standard Dextran
(6K)

Uptake by Mouse Macrophages
SPIO coated with amino-silane

Uptake by Mouse Macrophages
SPIO coated with lipids

Uptake by Mouse Macrophages
Lipid encapsulated SPIO coated with
amino-silane

Uptake by Mouse macrophages
SPIO coated with CS

Uptake by Mouse Macrophages
SPIO coated with Dextran (6k)

Slide shows of uptake of spio by macrophages and monocytes10

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Slide shows of uptake of spio by macrophages and monocytes10

1. Uptakes of SPIO by Murine Macrophages and Monocytes Daniel Chan University of Colorado Health Sciences Center 12-19-2001

2. Synthesis of SPIO • 1. SPIO coated with chondroitin sulfate • 2 SPIO coated with standard dextran (6K) • 3 SPIO coated with Amino-silane • 4 SPIO coated with lipids • 5 Lipid encapsulated SPIO coated with amino-silane

3. Uptakes of SPIOs by murine peritoneal macrophages and monocytes • Normal mice were treated with mineral oil by ip injection. • 24 hours later, equal amount of various SPIOs were injected intraperitoneally. • 24 hours later, peritoneal macrophages and monocytes were isolated. • Uptakes of various SPIOs were examined by light microscopy.

4. Uptake by Mouse Macrophages SPIO coated with chondroitin sulfate

8. Uptake by Mouse Macrophages SPIO coated with standard Dextran (6K)

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12. Uptake by Mouse Macrophages SPIO coated with amino-silane

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16. Uptake by Mouse Macrophages SPIO coated with lipids

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20. Uptake by Mouse Macrophages Lipid encapsulated SPIO coated with amino-silane

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24. Uptake by Mouse macrophages SPIO coated with CS

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27. Uptake by Mouse Macrophages SPIO coated with Dextran (6k)

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30. Uptake by Mouse Macrophages SPIO coated with Amino-silane

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33. Uptake by Mouse Macrophages SPIO coated with lipids

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36. Uptake by Mouse Macrophages Lipid encapsulated SPIO coated with amino-silane

Slide shows of uptake of spio by macrophages and monocytes10

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