Analytical Techniques in Biotechnology - Topics and Syllabus

1. BM3002: Analytical Techniques
in Biotechnology {3-0-0 / 3}
Course Instructor: Dr. Kunal Pal (8249247377)
Course TA: Mr. Deblu Sahu (6264446673)

2. Topics to be covered
• Spectroscopic techniques: Electromagnetic spectrum and Absorption spectroscopy,
Ultraviolet and visible light spectroscopy, Fluorescence spectroscopy, Luminometry,
Circular dichroism spectroscopy, Light scattering, Atomic spectroscopy, Infrared and
Raman spectroscopy, Surface plasmon resonance, Electron paramagnetic resonance,
Nuclear magnetic resonance, X-ray diffraction
• Microscopy: The light microscope. Phase contrast, confocal, Electron microscopy: TEM &
SEM
• Chromatographic techniques: Principles of chromatography, Chromatographic
performance parameters, High-performance liquid chromatography, Adsorption
chromatography, Partition chromatography, Ion-exchange chromatography, Molecular
(size) exclusion chromatography, Affinity chromatography, Gas chromatography.

3. • Mass spectrometric techniques: Introduction, Ionisation, Mass analysers,
Detectors, Structural information by tandem mass spectrometry, MALDI-
TOF
• Electrophoretic techniques: General principles, Support media,
Electrophoresis of proteins, Electrophoresis of nucleic acids, Capillary
electrophoresis, Microchip electrophoresis.

4. Grade evaluation
• 4 class tests  Average of best 2 CTs (5 marks)
• 1st CT = /20
• Maintaining class notebooks  (5 marks)
• 1 check #
• Home assignments  (5 marks)
• Assignment 1 – done
• Assignment 2 - pending
• Flipped classes  (5 marks)
• Mid + End- semester examinations  (80marks)

5. Books
• Instrumental Methods Of Analysis; H. H. Willard, L. L. Merritt, J. A.
Dean, F. A. Settle; Publisher : CBS; 7edition (1 December 2004)
• Instrumental Methods of Chemical Analysis; G. R. Chatwal, S. K.
Anand; Himalaya Publishing House (1 January 2014)
• Instrumental methods of analysis; K. R. Mahadik, L. Sathiyanarayanan;
Publisher: Nirali Prakashan (1 January 2020)