Types of Pathogenic Organisms
Viruses
Bacteria
Protozoan
Fungi
Animal
Parasites
mecahnism
Utilization of host nutritional resources
Physical damage to host tissues
Production of toxic substances
Chromosomal and gene damage
Body cells behave abnormally
Antigens
Some chemical that creates immune response
Most are proteins or large polysaccharides from a foreign organism.
Microbes: Capsules, cell walls, toxins, viral capsids, flagella, etc.
Nonmicrobes : Pollen,, serum proteins, and surface molecules from transplanted tissue.
Antigens
Some chemical that creates immune response
Most are proteins or large polysaccharides from a foreign organism.
Microbes: Capsules, cell walls, toxins, viral capsids, flagella, etc.
Nonmicrobes : Pollen,, serum proteins, and surface molecules from transplanted tissue.
Skin acts as barrier to microbes and viruses
- sweat has a low pH
Mucus traps foreign particles
Tears
- Lysozyme has antimicrobial action
Gastric stomach acid
2nd line of defence
Phagocytic cells (WBCs)
Natural Killer (NK) Cells: attack virus infected cells
Inflammatory Response
Antimicrobial proteins
Lysozyme
Interferon
Antibodies
3. MECHANISMS OF
DISEASE BY
PATHOGENS
• Utilization of host nutritional
resources
• Physical damage to host tissues
• Production of toxic substances
• Chromosomal and gene damage
• Body cells behave abnormally
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6. Antigens
Some chemical that creates immune response
Most are proteins or large polysaccharides from a foreign
organism.
Microbes: Capsules, cell walls, toxins, viral capsids, flagella, etc.
Nonmicrobes : Pollen,, serum proteins, and surface molecules from
transplanted tissue.
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7. Antigens
Epitope:
Small part of an antigen that interacts with an
antibody. 10-12 amino acids
Any given antigen may have several epitopes.
Each epitope is recognized by a different
antibody.
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10. • Skin acts as barrier to microbes and viruses
- sweat has a low pH
• Mucus traps foreign particles
• Tears
- Lysozyme has antimicrobial action
• Gastric stomach acid
1st Line of Defense
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17. CHARACTERISTICS OF
IMMUNITY
• Response is specific
• Retains a “memory” allowing an
accelerated second response
• Can respond to many different
materials
• Involves lymphocytes and antibodies
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18. TYPES OF
IMMUNITY
• Active Immunity
- Naturally-Acquired Active Immunity
- Artificially-Acquired Active Immunity
• Passive Immunity
- Naturally-Acquired Passive Immunity
- Artificially-Acquired Passive Immunity
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20. ACTIVE IMMUNITY
The production of antibodies against a specific disease by the immune system.
Naturally acquired through disease
Artificially acquired through vaccination
Vaccines include inactivated toxins, killed microbes, parts of microbes, and viable but
weakened microbes.
Active immunity is usually permanent
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21. PASSIVE IMMUNITY
Passive Immunity- Protection against
disease through antibodies produced by
another human being or animal.
Effective, but temporary
Ex. Maternal antibodies.
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22. Passive immunity can be transferred artificially by injecting antibodies from an
animal that is already immune to a disease into another animal.
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23. IMMUNE SYSTEM RESPONSE TO
ANTIGENS
Humoral Immunity
• Involves antibodies (secreted from B
cells) dissolved in the blood plasma.
• Demonstrated as a immune response
using only the blood serum.
• Defense against bacteria, bacterial
toxins, & viruses.
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24. Cell-Mediated Immunity
• Involves the activities of specific white
blood cells (T cells).
• Defense against cancer cells, virus-
infected cells, fungi, animal parasites, &
foreign cells from transplants.
IMMUNE SYSTEM RESPONSE TO
ANTIGENS
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26. B CELLS
• Mature in bone marrow
• Involved in humoral immunity
• Once activated by antigen, proliferate
into two clones of cells: plasma cells
that secrete antibodies and memory
cells that may be converted into plasma
cells at a later time
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31. Antibodies
Proteins that recognize and bind to a particular
antigen with very high specificity.
Made in response to exposure to the antigen.
One virus or microbe may have several antigenic
determinant sites, to which different antibodies may
bind.
Each antibody has at least two identical sites that
bind antigen: Antigen binding sites.
Belong to a group of serum proteins called
immunoglobulins (Igs).
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32. Antibody Structure
Monomer: A flexible Y-shaped molecule with four protein
chains:
2 identical light chains
2 identical heavy chains
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34. MECHANISMS ON ANTIBODY
ACTION
• Precipitation of soluble antigens
• Agglutination of foreign cells
• Neutralization
• Enhanced phagocytosis
• Complement activation leading to cell lysis
• Stimulates inflammation
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35. The binding of antibodies to antigens to form antigen-antibody complexes is the
basis of several antigen disposal mechanisms.
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36. Immunoglobin Classes
IgM
• 1st response to antigen
• Effective in agglutination
• Can’t cross placenta
IgG
• Most common form
• Crosses blood vessels
• Crosses placenta (passive
immunity to fetus)
• Provides immunity to new
born babies
IgA
• Secreted from mucus membranes
• Prevents attachment of bacteria
to epithelial surface
• In colostrum
IgD
• B cell activation
• Can’t cross placenta
IgE
• Histamine reactions
and allergies
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38. T CELLS
• Mature in thymus
• Involved in cell-mediated immunity
• Activated when another cell presents
antigen to them
• Several types of T cells: cytoxic T cells,
helper T cells, suppressor T cells, and
memory T cells
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39. IMMUNOLOGICAL PRODUCTS
NAME TYPE Immunity Dose
BCG vaccine (Bacillus
calmette guerin)
Live attenuated
Strain of bacillus of calmette and
guerin
active Intracutaneous
0.1ml
Cholera vaccine Killed cholera vibrios(Vibrio
cholerae(Strain of inaba and
Ogawa)
active S/C 1st dose:0.5ml
2nd dose:1ml
Diptheria
antitoxin
Plasma or serum of healthy
horse
Cornybacterium diptheriae
freeze dried powder
passive 10000-30000 units
I/M in severe cases
40000 units
Diptheria and tetanus toxoid C. Diptheriae and C. tetani
Adsorb formaldehyde treated
diptheria and tetanus
toxoid(nitrogen purified) on
Al(OH)2
active 0.5ml by I/M inj.
0.5ml after 4 weeks
Diptheria, Tetanus and
pertusis vaccine
Purified diptheria and tetanus
formol toxoid +killed Bordetella
pertusis
active I/M inj 0.5ml each
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40. NAME TYPE Immunity Dose
Gas gangrene
antitoxin
Clostridium oedematiens
Clostridium perfringens
Clostridium septicum
Antibodies from horse serum
passive 10000 units -
30000 units by
I/M inj
Hepatitis B vaccine Formalin inactivated Hepatitis B
virus
active 20µgl/M in 3
doses
Human normal
immunoglobulin
Sterile solution or freeze dried
product containing
immunoglobulins mainly IgG
passive 250mg I/M inj. -
750mg by I/M inj.
Measles vaccine Freeze dried pdt containing live
attenuated measles virus in chick
embryo
active 0.5ml by
subcutaneous inj.
Plaque vaccine Killed yersinia pestis, 195P strain
grown on enriched media like
acid hydrolysate of casein , killed
by addition of formaldehyde
passive 1ml by SC inj.
Rabies antiserum Antirabies globulin(horses) passive 1st dose by I/M
then 2nd by SC inj
Rabies vaccine
human(cell culture)
Frreeze dried product of rabies
virus inactivated by
betapropiolactone
Fertile eggs
active I/M inj in 2.5
units
Rabies vaccine Rabies virus injected
intracerebrally in brains of
animals, virus harvested and then
suspended in sodium chloride
active 1-5ml daily for
7-14 days by SC
OR IM ROUTE
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41. NAME TYPE Immunity Dose
Shick test toxin Cornybacterium diptheriae.
Toxin is purified ,diluted with
sterile aqueous buffer
solution of pH 7.2-7.6 ,made
preparation isotonic with
blood
Test to detect
diptheria
0.1-0.2 ml by
intradermal inj.
Typhoid vaccine Killed by heat or
bactericide(PHENOL/FORMALD
EHYDE) or by chemicals as
acetone
Salmonella typhi
active 0.5-1ML (4-6 weeks)
Typhus vaccine Killed typhus rickettsiae
(yolk sacs of fertile egg) or
lungs of small rodents
Peritoneal cavities of gebrils
active 0.25-1ml by SC ROUTE
Japanese encephelitis
vaccine
Japanese encephelitis vaccine
strain intracerebrally in mice
brain (freeze dried product)
active 1mlX2 at 1-2 week
interval
Poliomyelitis vaccine Attenuated strains of polio
virus
active 0.1ml-2 drops
Tetanus vaccine Sterile sispension prepared
from tetanus toxoid adsorbed
on aluminum phosphate in
saline solution
active 2 I.M. injections of
0.5ml each
Viper Venom Dried secreation obtained
from poison glands of Viperae
russelli, secreation dried from
Mouse unit dose 1-3
with initial dose 5-25
units 41
42. Tetanus
antitoxin
Antoxic globules
derived from
horse serum
passive By SC or IM
injection
NLT 1500 units-
50000 units
(Passive
immunity)
Yellow fever
vaccine
Live aqueous
suspension of
chick embryo
tissue injected
with 17 D stain
of yellow fever
virus
active 0.5ml by SC
route
Scorpion venom
antiserum
Sterile
preparation
containing
anttoxic globules
of venom of
scorpio(serum of
healthy equines)
20-50ml inj by
IV route
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