This presentation was made in Sept 2010 at Manila during the Poultry show. Target audience were nutritionists , poultry consultants and feed manufacturers

1. PHYTOCONSTITUENTS IN ANIMAL FEED AN OPPORTUNITY Dr (Capt) Tanweer Alam INDIA

3. Total number of research papers listed on PubMed containing the word "phytoconstituents."

4. How the usage of Phytoconstituents based products have progressed ?

6. Conventional Approach of Phytoconstituent based product development: Ethno-medical information Development of prototype formulations Validation of safety & efficacy using invivo models Chemical fingerprinting Marketing

9. Factors affecting Sec Metabolites? Secondary Metabolites Part used Geographical factor Period, time & method of collection Storage Water availability Drying Packing and Transportation Age of the plant

13. Indian Herbal Pharmacopoeia British Herbal Pharmacopoeia English Translation of German Commissions E monographs American Herbal Pharmacopoeia ( AHP ) World Health Organization (WHO) Europe Scientific Cooperative on Phytotherapy (ESCOP) United States Pharmacopoeia ( USP ) Confluence of Herbal Pharmacopoeia

15. Pharmaceutical Research and Development (R & D) Process Definitions IND = Investigational New Drug Application FDA = U.S. Food and Drug Administration NDA = New Drug Application Source: www.Innovation.org

24. GLOBAL QUALITY TESTED & TRUSTED India Malaysia Japan USA & Italy

25. Country - Malaysia Field trial in commercial broilers (AA) – Dr.V.Raghavan, Seng Heng Chan Feed

26. Country - Japan Field trial in commercial broilers (Chanky) – Kohara Feed & Breeding Co. Ltd. Poultry Farm, Wakayama

27. Country - USA Field trial in commercial broilers – Dr. Danny Hooge, Utah State University, Logan –UT

28. Country - Italy Field trial in commercial broilers - Dr.Schienoni Milano

29. Source : World Poultry - V.15 # 11, 1999 MYCOTOXINS - A WORLDWIDE SCENARIO “ Food and Agriculture Organisation (FAO) of the UN estimated that 25% of the world's grain supply is contaminated with Mycotoxins ” . Liver in a good condition The target organ for the mycotoxins and attack of other chemical toxicants is LIVER . Liver in a good functional condition is very useful for basic detoxification mechanism and overall digestion & metabolic functions of the body. Zigbir has potent effect on the development & efficient functioning of liver under normal and challenged condition. MYCOTOXINS

30. HEPATOPROTECTION (Against FUNGAL TOXINS) Prof.D.Narahari,Ph.D., Prof. & Head, Poultry Science, Vety.College, Chennai

31. HEPATOPROTECTION (Against FUNGAL TOXINS) GROSS LESION - LIVER (e) ND H.I Titers BETTER FEED & FEEDING Gross pathology of liver of challenged untreated group (A) reveals hepatomegaly, severe discoloration, blunt edges and fragility. While the gross pathology of liver of the challenged treated group (B) is closer to the normal.

32. HEPATO PROTECTION (Against CHEMICAL TOXICANTS) Dr.Deben Sapcota Associate Professor Dept. of Poultry Science Assam Agricultural University

33. Utilization of Xanthophill Roche colour fan index basis

37. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Emblica officinalis Part used : Dried Fruits Main Uses : Immunomodulatory and Antioxidant (Suresh & Vasudevan, 1994). Active principle : Gallic acid Gallic acid Ursolic Acid Part used : Whole plant Main Uses : Immunomodulatory, Antistress (Sen et.al.,1992) Active principle: Ursolic acid Ocimum sanctum

38. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Emblica officinalis Gallic acid Chebulic acid Ellagic acid Chebulinic acid

39. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Known to be active in scavenging DPPH*, Superoxide anion * DPPH : diphenyl picrylhydrazyl (free radicals), turns colorless when reduced by antioxidant.

41. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Potent Anti-oxidant Under stress free condition Phytocee-Under stressful condition A.T.Ps. Energy Free Radicals Scavenged S.O.D. More energy More A.T.Ps. Extra Free Radicals (> normal) Destroyed by Antioxidants The phytoactives of Phytocee possess a potent ability to donate H+ atom and acts as a natural anti-oxidant.

42. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Phytocee shows a potent free radical scavenging activity very similar to L-ascorbic acid (Vitamin 'C'). SCIENTIFIC VALIDATION: Anti-oxidant Activity Principle: DPPH [1,1-diphenyl-2-picryl hydrazyl] is a stable free radical with purple colour, the intensity of which is measured at 510nm spectrophotometrically. Antioxidants reduces DPPH to 1,1-diphenyl-2-picryl hydrazine, a colourless compound. 1, 1-diphenyl-2-picryl hydrazyl Purple colour 1, 1-diphenyl-2-picryl hydrazine Colourless Effect of L-Ascorbic acid & Phytocee on free scavenging activity Phytocee L Ascorbic acid

43. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Oxidative stress (OS) is one among the common stressors of cells. OS can result in membrane damage leading to several pathological effect. Reference: Chandarasekaran C.V., et al , Invitro efficacy and safety of poly-herbal formulations. Toxicol, invitro (2010), doi:10, 1016/j.tiv.2009, 11, 021. As per various research Phytocee exhibits an appreciable cellular anti-oxidant effect.

44. Herbal Poultry Feed Supplement for Replacing Synthetic Vitamin C Phytoactives of Phytocee Possess a proven immuno-modulating property against bacterial as well as viral infections An Immunomodulator Conclusion The results indicate that, both the products i.e., Phytocee and Vitamin-C (L-Ascorbic acid) possess the same macrophage phagocytosis stimulating activity. Therefore, Phytocee can replace Vitamin-C cent percent and restores the immune status of the bird under any stress or infective conditions. Effect of Phytocee and Vitamin 'C' on macrophage phagocytosis Phytocee / Vit. C (µg/ml) % Infected macrophages 0 100 500 83 ± 4 100 ± 0 100 ± 4 - 1.2 1.2 - 1.1 1.2 83 ± 4 96 ± 1 100 ± 1 Phytocee Vit. C Phytocee Vit. C

47. More Phytoconstituents options For Replacement of Synthetic Methionine & Equivalent