Dna Extraction From Wheat Germ

DNA Extraction from Wheat Germ Compliments of the Salk Institute and the Southern CA Biotechnology Center at Miramar College

In This Lab You Will Use Plant Cells found in Wheat Germ You will need to break open the Cells to get the DNA How many layers do you need to go through to get to the nucleus?

Your Lab Set Up Should Look Like This- Find the Wheat Germ

The Yellow Label is Isopropanol What is the Green Stuff?

Get your Wheat Germ (Plant Cells) ,[object Object],[object Object]

Think! HINT: Wheat Germ comes from plants but the Water is removed What do you think is happening to the wheat germ ?

Break Open the Cells ,[object Object],[object Object]

Heat to Release DNA ,[object Object],[object Object],[object Object]

While the Wheat Germ is Heating Get a Tube Ready for Your DNA and Think About What Will Happen Next ,[object Object],[object Object]

The Next Step is to Extract the DNA ,[object Object],[object Object],[object Object],[object Object]

Precipitate the DNA with Alcohol ,[object Object],[object Object],[object Object],[object Object]

DNA is Hydrophobic and Moves into the Alcohol Layer ,[object Object],[object Object]

DNA forms at Alcohol-Soap Interface- Spool DNA Onto Loop

Pick Up the DNA for Transfer into the Small Tube

Isolate the DNA into Your Small Tube ,[object Object],[object Object]

Clean Up ! ,[object Object],[object Object],[object Object]

Observe ,[object Object],[object Object],[object Object]

Dna Extraction From Wheat Germ

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