The document summarizes oxidative phosphorylation. It discusses that oxidative phosphorylation is the process by which ATP is synthesized from ADP and Pi coupled with the electron transport chain. This occurs at complex V of the inner mitochondrial membrane. The P:O ratio refers to the number of ATP molecules generated for each oxygen atom consumed or electron pair transported through the electron transport chain, with mitochondrial NADH oxidation having a P:O ratio of 3.

1. BIOCHEMISTRY
[B. Pharm – I Yr.]
Topic: Oxidative phosphorylation
Umesh Kumar
Assistant Professor
Dept. of Pharm. Chemistry
Hygia Institute of Pharmaceutical Education and Research

2. CONTENTS
• Oxidative phosphorylation
• Introduction
• Types
• Mechanism
• Hormonal Regulation

3. Oxidative phosphorylation
• The transport of electrons through the ETC is linked with the release of free energy.
• The process of synthesizing ATP from ADP and Pi coupled with the electron transport chain is known
as oxidative phosphorylation.
• The complex V of the inner mitochondrial membrane is the site of oxidative phosphorylation.
P : O Ratio
• The P : O ratio refers to the number of inorganic phosphate molecules utilized for ATP generation for
every atom of oxygen consumed.
• More appropriately, P : O ratio represents the number of molecules of ATP synthesized per pair of
electrons carried through ETC.
• The mitochondrial oxidation of NADH with a P : O ratio of 3 can be represented by the following
equation :

5. THANK YOU
HYGIA GROUP OF INSTITUTIONS
GHAILA ROAD, GAAZIPUR BALRAM,
FAIZULLAHGANJ, LUCKNOW