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growth of  Methanosarcina acetivorans  with carbon monoxide as the substrate Heather Jordan Department of Biochemistry and Molecular Biology The Pennsylvania State University University Park, Pennsylvania 16802
A Method to the Madness…
Hypothesized Pathway 1
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Hypothesized Pathway 2
 
 
 
 
 
 
 
 
 
 
 
 
 
 
Substrates ,[object Object],[object Object],[object Object]
Changing Substrates ,[object Object],[object Object],[object Object],[object Object]
Scrubbing the CO  (to remove traces of O 2  and CO 2 ) ,[object Object],http://www.calibredigital.com/Services/index.html
 
 
Withdrawing CO for Pressurization
Pressurizing the Serum Bottle
Greatest Surface Area
Results
Nothing!!! ,[object Object],[object Object],[object Object],[object Object],[object Object],Failure
Changes in Method: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],We shall wait and see what happens…
While waiting for the  mixed gas tank to show up… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
To be continued…
Creating ISF Knockouts! Boxing template from:  http://joyjobs.com/esl/tefl_1.htm Kevin’s head from: http://www.bmb.psu.edu/debtpage/ferrylab/evenmorepicturesa.html Coming Soon  To A Lab Near You
 
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Acknowledgments ,[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/kevin.html
Acknowledgments ,[object Object],[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/frank.html
Acknowledgments ,[object Object],[object Object],[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/lablist.html
Acknowledgments ,[object Object],[object Object],[object Object],[object Object],http://www.bmb.psu.edu/deptpage/ferry.htm
And Special Thanks To: ,[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/lablist.html
And Special Thanks To: ,[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/lablist.html ,[object Object]
And Special Thanks To: ,[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/lablist.html ,[object Object],[object Object]
And Special Thanks To: ,[object Object],[object Object],[object Object],[object Object],Sorry, no photo available (Cameron lab does not have a website).
And who could forget… ,[object Object],http://www.bmb.psu.edu/deptpage/ferrylab/lablist.html http://www.atpictures.com/display.php?path=/ben&loc=serva8&img=ba95.jpg
 
[object Object],The End 5   m

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Growth Of Methanosarcina Acetivorans On Carbon Monoxide Substrate