Shojinmeat Project : Clean meat initiative April 2018
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Shojinmeat Project : Clean meat initiative April 2018
- 1. Clean Meat Initiative (2018 edition)
- 2. “Democratization of cellular agriculture” Nonprofit non-corporate non-university citizen science community of DIY bio/fab enthusiasts, students, researcher, artists, writers etc. for cellular agriculture “Shojinmeat Project” - Who we are Open source cellular agriculture” by DIY bio Public communication by art and education
- 3. Ongoing projects 3 ・DIY bio & cell culture experiments ・Workshops and micro-conferences ・Advocacy for cellular agriculture ・Media and social communication ・Art, design and creativity projects ・Visual contents production ・Fundraising / crowdfunding
- 4. 4 “Meat”
- 5. From where? Meat is ~x40 more resource intensive Lamb：~x50, Beef：~x40, Pork：~x20, Poultry：~x7 “Meat”←animals←feed, water, land 5
- 6. Deforestation Public health hazard Water shortage “Meat”←animals←feed, water, land 6
- 7. 7 Food vs. Feed vs. Fuel Agri- cultural resources Food Feed Fuel
- 8. MeatSoy etc. Dairy MeatDairySoy etc. New alternative protein Plants Tofu Algae Insects Biosynthetic Cell culture New protein source “Meat & dairy produced in new ways” Plant-based meat & dairy equivalent Now Future “Alternative protein” and “meat alternative” 8
- 9. Cellular Agriculture Agricultural products by cell culture 9 Medicine technology, Agriculture application
- 11. Energy conversion efficiency 11 < 0.1% Microalgae: 4~11% “Artificial leaf”: 10%+ Ongoing improvements ~4% ~35% 50~90%
- 12. Ongoing cellular agriculture projects 12 Clean meat Milk without cows Egg white without eggs The products are not “imitations” - they are (or try to be) molecularly the same! Clean fish meat Ginkgo Bioworks - Vanilla, scent, various ingredients Modern Meadow - leather without animals
- 13. What cellular agriculture potentially achieves 13 Protein source with marginal resource input
- 14. Research track record 1 14 1997 Goldfish meat @NASA Appearance in sci-fi - Concept known since 19th century 2004 New Harvest founded Jason Matheny contacts NASA staff 2005 Netherlands funds €2M 2007 In vitro meat consortium funding discontinued funds 2000 Works by Oron Catts @Harvard Lead by Willem van Eelen (deceased) Patent filed in 1997
- 15. Research track record 2 15 2012 Sergey Brin from Google contacts former member of In vitro meat consortium 2013 Demonstration by Prof. Post 2014 Shojinmeat Project 2015 Memphis Meats 2015 “Cellular agriculture” - term coined 2013 New Harvest invests in cell-ag startups (Clara Foods, Perfect Day) 2016 SuperMeat 2017 Finless Foods €280k burger
- 16. 16 The positive impact 學 經 藝 Science & Technology ・Technological hurdles? ・Medical applications? Politics & Economics ・Shift in food market? ・Industry landscape? Arts and Culture ・Religious views? ・Social norms to change?
- 17. ￥28,000,000 Cultured burger, 200g ($260,000) The Problem 17
- 18. Labs, Hospitals Brewery, Petrochemical complex Culturing of cells has been optimised for laboratory scale ⇒€250k per burger Culturing of cells becomes industrial scale ⇒Production at $2/kg 18 Conditions and purposes change →Entire process needs a re-design
- 19. No 100mm dish but use 25㎥ tank No gloves but do tank sterilisation No pipettes but use pipelines If tanks are sterilised, no antibiotics needed Conditions and purposes change →Entire process needs a re-design 19
- 20. Technological milestones 20 1. Inexpensive media 2. Scaling & automation Conventional meat price parity? 3. Added value and consumer acceptanceCapEx & Staffing Culture medium $200k+/kg Conventional method $2 設備費・人件費 培養液 Technological goals 1. Food grade culture medium 2. Scaled culture plant design 3. Tissue engineering for flavor and texture
- 21. Bottlenecks in culture media 21 Gospodarowicz D and Moran JS, 1976, Annu Rev Biochem Eagle H, 1959, Science Sugar, Amino acids, Vitamins, Minerals $20/L Expensive for what’s actually in Albumin, Buffer, Insulin, Transferin $900/L “Mad cow”? Viruses? Expensive and supply is insecure Growth factors Survival factors $450/mg Expensive… :-( ＝ Basal medium Foetal bovine serum Signal compounds
- 22. Standard DMEM(FBS10%) 500ml $5000+ for 100g Medium for 1~2g of cells Cost of culture media (yen/JPY) 22
- 23. Block et al., 1996 DMEM 450ml ￥1125 FBS 50ml ￥4900 Non essential amino acid ￥140 HGF 40ng/ml ￥78000 (20µg) EGF 20ng/ml ￥700 (10µg) ￥84865($8000) 100g of liver cells cost ~$100,000 Liver cell culture for therapeutic applications 23
- 24. “Cooked medium” approach at Shojinmeat Project 24 Commercially available: “Yeast extract for animal cell culture” (Sheffield Bioscience Ltd.) Dried yeast (dog food) Phosphate buffer & salt Papain, 65℃, stir, ~24h to “digest” yeast Filtered before use in cell culture pH7.4 No bacterial contamination Preparation cost: 10¢/L
- 25. Myoblast cell response 25 The trial solution showed signs of L6 growth and multiplication. ⇒”Yeast extract (dog food) is a potential FBS alternative.” NOFBS Control (withFBS) Mouse L6 Myoblast cell density (qualitative) Red ~ Yellow ~ Blue(zero) No DMEM w/ DMEM No DMEM w/ DMEM Conc. yeast extract Dil. yeast extract
- 26. HeLa cells in yeast extract medium 26 HeLa cells in sYE (yeast extract) multiplied at 80% of that in FBS HeLa cells in sYE medium grew in diameter more than in FBS Cellcount(x10e5) (HeLa = Human cervical cancer cell line) Day0 Day7 FBS 10% sYE 10% sYE 10% + FBS 10%
- 27. 293T cells in yeast extract medium 27 sYE is capable of culturing 293T cells, which have relatively strict requirement for FBS to thrive Cellcount(x10e5) (293T = Human Embryonic Kidney cells) Day0 Day7 FBS 10% sYE 10% sYE 10%+ FBS 10% 10％ FBS 10％ sYE 10% FBS 10% sYE
- 28. Cellular self-organisation in sYE 28 96-well suspension culture plate Mouse myoblasts were multiplied to 1.0E5 in standard FBS medium Cells were placed under suspension culture condition in sYE medium for 4 days ⇒Self-organised into a 3-dimensional aggregate of 0.3~0.5mm diameter
- 29. The limits 29 Species Cell identity sYE conc. Result Human HeLa Human cervical cancer 10％ Successful (but for how long?) Human Human Embryonic Kidney 293T 10％ Successful (same as above) Mouse Germ cell 10％ Unsuccessful Mouse Ovarian somatic cell, primary 10％ Successful (but for how long?) Mouse Hepatocyte, primary 10％ Successful (same as above) Mouse Fetal cell, primary 10％ Successful (same as above) Mouse Myoblast, primary 10％ Initially successful, but cell division halts after ~3gen. Mouse Embryonic stem cells 10％ Unsuccessful Yeast extract do have limits. Unsuitable for undifferentiated cells.
- 30. Supplementing growth factors - Method 1 30 Glocose, amino acids, AA2-P, NaHCO3, Na2Se These (expensive) growth factors will be made by recombinants 20~200kL cell cultivator tank ~3t scale production Insulin, Transferin, FGF2, TGF-b “Essential 8” clean meat medium Growth factors & FBS ingredients “All-in-1 batch”
- 31. Cost of goods (ingredients) analysis 31 Medium Meat $41/L $100/lb $15/L $36.6/lb $4.7/L $11.5/lb $3.7/L $9.0/lb $0.77/L $2.2/lb Scenario A~E A: All GF’s down to 1/10 in cost B: FGF2 & TGFβ at insulin price C: A&B D: All GF’s at $4/g E: Basal medium at $0.23/L Good Food Institute (2016)
- 32. ⇒$0/L Originally $1500/L Produces growth factors Muscle cells Liver cells growth factorsGF’s & FBS eq’s Basal medium Inexpensive Very expensive Medium ingredients GF’s & FBS eq’s Supplementing growth factors - Method 2 “Coculture”
- 33. Demonstration of coculture method 33 Control (0%) 10% conditioned medium 25% conditioned medium 50% conditioned medium Countofcellsofallsizes, relativetothecontrol group Secondhand medium is effective! mouse placental cells dishes with Day-12 foetal liver cells in FBS 10% medium 7 Days Transfer culture medium
- 34. 34 Clean Foie Gras demonstration Grow cells Fat-load cells Assemble cells
- 35. Low-cost liver cell culture 35 DMEM 450ml ￥1125 Non essential amino acid ￥140 FBS 50ml ￥4900 HGF 40ng/ml ￥78000 (20µg) EGF 20ng/ml ￥700 (10µg) ￥84865(€750~) Liver cell aggregate on collagen scaffold Cultured by coculture in sYE medium DMEM 450ml ￥1125 sYE 50ml ￥182 ￥1307 (€12)
- 36. “Food grade” DMEM 36 DMEM 450ml ￥1125 sYE 50ml ￥182 ￥1307 (€12) ”DMEM” 450ml ￥10 sYE 50ml ￥1 ￥11 (€0.10) Sugar Amino acids Vitamins Minerals Basal medium = from algae?
- 37. Sports drink culture media 90% DAKARA 80% 70% 60% 0% (DMEM only) 50% Days Celldivisions Proliferation of mouse L6 in DMEM/GreenDakara 10% FBS #pH and osmolality of Dakara adjusted by NaHCO3(s) and 2M NaOH Fluid name osmolality pH DMEM(-, hi glu) 345 7.4 Pocari 338 3.4 Aquarius 291 3.37 Amino-Value 4000 289 3.63 AminoVital Gold 186 3.33 Vitamin Water 302 3.3 Green DAKARA 322 3.28 Amiiru Water 249 3.4 Mamoru Chikara 546 3.58 37
- 38. DIY-DMEM (home made medium) 38 Glucose, amino acids, vitamin B’s, salts Mix and filter to prepare Chicken foetus heart cells primary culture http://animescience.net/?p=3647
- 39. Scaling as it is... 39 How cultured burger was made in 2013 ⇒$260,000
- 40. Not big enough if for food 40 Conventional “scaled” cell culture
- 41. Dr. Marianne Ellis, 2017 “Process scale” method implementation cases⇒ “Culture methods suitable for scaling”
- 42. Integration of all elements into one scalable system ~0.1g scale ~10g scale ~100kg scale future pilot plant Ready for plant engineering? 42 PCT/JP2016/067599 jp-pat file# 2016-568716
- 43. How is temperature controlled? Mixing method? Pipeline diameter & flow rate? Sterilization method & frequency? How are filters cleaned? Plant engineering - what exactly? Speculative fish meat culture plant
- 44. “Is it tasty?” - Tech to add value 44 Sausage/burger Proven ※although expensive Low cost large scale cell culture Sheet meat / “bacon” Cell scaffold Muscle/fat coculture Steak / tissue Tissue morphogenesis Vascularization Meat texture Where we are Regenerative medicine
- 45. Culture medium and starter cells Myoblast culture Myogenesis, Cell organization Grow cells Make tissue by cellular scaffoldProcurement How to make clean meat : method 1 45
- 46. Cellular scaffold also achieves the advantage of 3D culture Sponge collagen scaffolds Liver cells on scaffolds 3D culture by cellular scaffolds 46
- 47. Edible scaffolds i.e. collagen, chitosan, chitin, arginate, cellu- lose, polysaccharides Simulate fibre and meat texture Moulds shape in mm or bigger scales Other benefits of scaffolds 47
- 48. Culture medium and starter cells Procurement How to make clean meat : method 2 Cell growth, vascularization Tissue growth ※Further down the development timeline Integriculture Inc. is in collaboration with Tokyo Women’s Medical University on tissue engineering 48
- 49. Tissue engineering, regenerative medicine and cellular agriculture Cell culture (human) Cell culture (animals) Distribution Distribution Regenerative medicine Cellular agriculture Med. Ag. Procu- rement Culture medium Cell-ag and Regen. Medicine share the same technology. Main differences are in purity, traceability and regulations 49
- 50. 50 The positive impact 學 經 藝 Science & Technology ・Technological hurdles? ・Medical applications? Politics & Economics ・Shift in food market? ・Industry landscape? Arts and Culture ・Religious views? ・Social norms to change?
- 51. Global meat $1.5T Global beef $0.7T Japan $50B Toward “price parity” Startups Speci- aliszed Funds NPOs Key start-ups and NPO’s
- 52. Global protein market outlook 52 Population growth and emerging economies: $2T market cap. by 2030? Global meat demand 1980-2030 Rabobank (2011)■Lamb ■Poultry ■Pork ■Beef Demand,10milliontons
- 53. “Protein problem” in different stages 53 Is there enough protein? Is it a secure source? Is it sustain- able? Many rely on imports while overfishing continues ( Economic and Social Research Institute, Dublin, Ireland, 2010 https://www.esri.ie/pubs/WP340.pdf) “Public expenses due to meat is set to reach $1.6T by 2050” Farm Animal Investment Risk and Return Initiative Poor conturies
- 54. How cellular agriculture is a solution 54 Meat supply chain Externalities “Wicked problem” of climate change, poverty and local ecological losses
- 55. How cellular agriculture is a solution 55 1 2 Reduce protein consumption. Change the way we eat. Find sustainable protein source. Preserve culinary culture. Meat supply chain Externalities “Wicked problem” of climate change, poverty and local ecological losses
- 56. Comprehensive cost and footprint assessment ・Resource requirement from ‘cradle to the grave’ Life cycle assessment (LCA)
- 57. LCA uncertainties 57 Current LCA estimates depend on unestablished production processes and uncertainties still may exceed 50%. 2014 estimate included bioreactor sterilization. Hanna Tuomisto 2011 Hanna Tuomisto 2014
- 58. “Matter cycle” of cultured meat Convert to culture media Large-scale cell cultureWaste fluid treatment Algal production Algae Culture media sewage Fertilizer
- 59. 59 “Adjacent industries” Entry from nearby fields Beverage companies Food companies Medical supply manufacturer Plant engine- ering firms Effect to nearby fields Functional & pharmaceuti cal foods Food branding Biomanu facturing Regen. medicine Indoor farms Clean meat industry mind map
- 60. 60 Political and regulatory environment 2013 amendment on biotechnology mentions food applications FDA conducts hearings from NPO’s in 2016 Set to spend €400M on alternative protein research from 2018 €140M is to be spent on biotechnology. Set to invest $300M on Israeli alternative protein startups (summer 2017) No active discussions yet due to lack of significant players in cellular agriculture.
- 61. 61 Food regulations (Japan) Clean meat is a Feed Safety Act Drug Safety Act Food Safety Act Food Labeling Act ＋Decision by food safety committee? 2018~ HACCP standard Consumers Only food and recognized food additives are allowed ⇒Antibiotics and cell growth factors have regulatory hurdles Raw meat? Processed meat?
- 62. 62 Food regulations (US) General background ・New product⇒GRAS or Food Additive Petition ・USDA for meat, FDA for fish, FDA for additives ・Feed has whole set of different regulations Precedent of enzymatic additives ・Regulatory issues in the use of recombinants in enzymatic additives production was raised before. ・Whole industry collaborated to get over. Other considerations ・Quicker to come up with its own toxicity test results ・Enzymatic additive is still distant as a “precedent”. ・Need a lobby to reach both FDA and USDA In setting a regulatory precedent for cellular agriculture products, “cultured fish meat as additive” is probably the most accessible. #Discussion at New Harvest 2017, NYC, 12.Oct.2017
- 63. Would vegetarians eat? Is it Halal? Goes with Buddhist “nonviolence”? Animal welfare? Consumer acceptance? At the end, is it tasty? Socio-cultural implications 63
- 64. A. Animals, religion B. Food security C. Food safety D. Env. footprint EU/US： A~D > C > B E.Asia： B~C > D > A Regional differences in agenda 64
- 65. Personal beliefs than religions ・Vegetarians ・Pescetarians ・Vegans are the most common Western vegetarianism 65
- 66. “Max. happiness for the max. number of sentient beings” ※Not “ends justify means”, but an guide to arguments over what’s ethical “Only 1 death is better than 5”...? May be so in very short term, but... What if the person is your brother? A society where abandoning of family is justified - would it be a good one? If “killing for public good” becomes the norm, no one is there to stop dictators - would it do “max. happiness for max. number of people in the long run? Classic “Runaway trolley problem” As it is, 5 will die, but if switch, 1 dies. Would you switch? Practical ethics: “Utilitarianism” 66
- 67. “Animal welfare” deduced from utilitarianism 67 All interests are equal: “1 person, 1 vote” No being’s happiness is more important than another ◆Are animals capable of experiencing happiness? ◆Should an animal count as one sentient being? In “animal welfare”, speciesism is dismissed & animals count as beings that can experience happiness ⇒From a utilitarian point-of-view, “making a sentient being suffer is unethical”
- 68. Acknowledges health and environment, but mainly animal welfare and ethical “Animal welfare” based on utilitarianism Reasons for vegetarianism 68
- 69. Announced in 2008 ⇒ taken down on 2014: technology wasn’t ready $1M Award by PETA 69
- 70. ”Religion”←Not utilitarianism or other ethics Ethical value of clean meat described by utilitarianism don’t directly translate into East Asian religious (i.e. Buddhist) importance. East Asian vegetarianism 70
- 71. Cuisine for for zen practitioners ・Common name for “Buddhist cuisine” ・All aspects must serve the purpose of zen. -NO WASTED FOOD -Use local produce to avoid food waste -No cruelty (avoid fish and meat) -Avoid certain vegetables that stimulate desires -All donated food including meat must be consumed -Preparation (cooking) is also part of zen practice Clean meat being “murderless” does not make it Zen - there are multiple more important criteria. “Shojin ryori” Buddhist cuisine ※contested “Shojin” means “devotion to the righteous path” Shojinmeat Project will stay committed to the path that obsoletes unsustainable meat
- 72. Holy text ↓ Rules ↓ Daily life Religious rules in Abrahamic religions RulesMiddle way Daily life Holy texts ”Would clean meat serve the pupose of Zen?” ・Overcoming personal desires is a major theme in zen ・”Desire” includes meat apetite ・”Fake meat” is a compromise, but compromise is permissible (refer. “middle way”)⇒ ・Clean meat falls in the same category as fake meats i.e. tofu. Use as reference up on making decisions Mentions Religious rules in Eastern religions “The Middle Way”
- 73. Before 7th century： Eating meat was common. People just had to eat whatever was in hand. 675c. Imperial decree of “No Killing (of animals)” To direct labour force to rice production and put a stop to local animal-sacrifice rituals & reinforce imperial authority ※Newly arrived Buddhism was used as justification Meat avoidance continues till 19th c. and commoners only started eating meat around 1900 c. Meat in historical Japan
- 74. ・Totals half billion? Region-specific ・More common among upper castes ・Some upper caste members fund cultured meat research ・Hinduism doesn’t explicitly forbid meat but adherents choose to avoid meat. Mr. Modi (Indian PM as of 2017) is a vegetarian. Vegetarianism in India 74
- 75. Then what if on 2040, meat alternatives rise to 30% market share and ever more people stop consuming conventional meat? ・Uncontrollable ”hate campaign” against traditional farmers? ・Trade ban of conventional meat due to animal cruelty? Why are animal experimentation, Japanese whaling and Chinese cat/ dog consumption is problematised far more than factory farming? ⇒Because they are “remote things” for the protesters. Possibility of “New Ethics”
- 76. (Transient) issues upon R&D： ・FBS production is not cruelty-free “Unavoidable” issue： ・Extraction of cells Likely to be solved in the future May pose an issue during R&D Can bovine foetus feel pain? It may still inflict some pain. Will animals still be chained? Genetic selection of animals for the sake of “tasty” - is it eugenics? Ethical issues due to technological immaturity
- 77. ・Can countries with meat culture technology blame others for animal abuse? ・”Ethical patent infringement” to stop “large-scale systematic animal cruelty”? Case study: Generic HIV drug lawsuit: An Indian pharmaceutical company allegedly infringed retroviral drug patent to manufacture generic HIV drugs, because the original drugs by Western pharmaceutical companies were too expensive for people in poor African countries. After high-profile court-martials, the Indian company won the case on humanitarian basis. Patent war under “New Ethics”
- 78. Chris Bryant (2017) 3rd Intl’ Conf. for Cultured Meat Large variations exist between different marketing research attempts and speculations, mainly due to the lack of actual clean meat products on market. ←Factors consumers weigh Consumer acceptance research result→ Consumer acceptance
- 79. Nomenclature - “clean meat”? Universal agreement has not been reached, but studies suggest that at least “cultured meat” is not a desirable option. →”Clean meat” is being suggested by advocates. And some suggest that name is not the most important issue after all...
- 80. All about “price & experience” in the end? $300,000/kg 2013 2019 20252022 Clean Foie Gras $40/kg “Designer Meat” $20/kg $/kg 2027 i.e. “fish fat beef” “superfood algae pork” “DHA/Ω3 boosted meat” General meat $6/kg Ingredients $3000/kg Plans to market different products according to unit production price Test production year
- 81. Highly personalized and “democratized” Even the largest corporations have <5% share 100’s of thousands of brands Millions of cafe&restaurants ⇒Rich of foodie culture Numberindemand Degree of personalization The food market
- 82. What would a corporate monopoly do? Resulted in widespread accusation and allegations of : “Technology being used for corporate profit than social good” “Public mistrust due to technological secrecy”
- 83. “Citizen science and business” at Shojinmeat Project How to make ⇒ Open How to scale ⇒ Proprietary Numberindemand Degree of personalization Product dev. Citizen Science domain Business domain and other businesses to come
- 84. Cellular agriculture ecosystem 84 Advocacy, Academic research with universities DIY bio, speculative art projects, “avant-garde” advocacy Sponsor Research and project grants Commercialization, Production technology development Individual biohackers in communities such as
- 85. Shojinmeat Project as citizen science 85 Non-Profit organization (TBC) Citizen Science Advocacy, Academic research with universities DIY bio, speculative art projects, “avant-garde” advocacy Sponsor Research and project grants (A spin-off startup) Commercialization, Production technology development
- 86. 86 Academia hints the way Citizens act and set the direction Businesses scale and deliver “Democratized cellular agriculture”
- 87. 87 “Growing meat at home” =DIY bio methodology= Konjac cell scaffold Cells from fertilized eggs Egg white as antimycotic Egg yolk as FBS ⇒DIY clean meat in kitchen High school girl on DIY clean meat experiment, TV news DIY clean meat recipe ‘Zine (2017 Comiket92)
- 89. 89 DIY Biomanufacturing Tasty originally designed meat DIY kidney!! ヽ(・∀・)ノ Homemade heart by DIY cell growth & differentiation New category of “meat” “Green meat” Algae meat mix ・Advanced home incubators allow DIY distributed biomanufacturing ・DIY tissue engineering is widespread and innovations occur everywhere
- 90. 90 Local farmers design meat ・Local farmers develop their own clean meat recipe to build local meat brands. ・Cattle breeders carry more value for the starter cells. ・Individual hobbyists follow to make their own recipe.
- 91. 91 “Beef” branded on individual cows Cell source cow still alive
- 92. Meat brewery 92 Myoblast grown at 200t scale
- 93. Steak brewery 93 Single use culture bags⇒ Steak grows inside⇒ Ready to ship⇒
- 95. Farmscape, A.D.2203 95 Vertical farms Vast land reverted to nature Artificial photosynthesis Cellular agriculture manu- facturing industry complex
- 96. Mars Colony 96
- 98. Members participate in their respective expertise (experiments, gatherings, art projects etc.) #Food Security #Food Miles #Regulations #Cooking #Culture & thoughts #History #Food safety #Life ethics #Animal welfare #Regenerative medicine #Tissue engineering #Bioreactor #Culture medium #Commercialization #LCA #R&D #Soc.&Econ. Shojinmeat “Distributed Clusters” 98 #Global collab. #Space #Art
- 99. Patrons at Campfire Crowdfunding All Shojinmeat Project participants! Acknowledgements
- 100. Miyo-san ”Myosin” age.20・164cm Chemical engineering student intern at Mars Huygens Crater cellular agriculture plant Aco-chan ”Actin” age.13・149cm Helps elder sister Miyo as part of extracurricular activity of Mars Colonists Middle School. Miyo & Aco
- 101. Miyo-san (Chibi)
- 102. Aco-chan (Chibi)
- 104. Contacts 104 http://shojinmeat.com info@shojinmeat.com http://integriculture.jp info@integriculture.jp http://scigra.com info@scigra.com Scientific VR, 3D printing and rendering service - funds Shojinmeat Project http://lne.st Seed accelerator