Green Flourescent Protein. Info about remarkable discovery of the century

1. GREEN FLUORESCENT
PROTEIN

2. GFP STRUCTURE
 A PROTEIN COMPOSED OF 238
AMINO ACIDS
 EXHIBITS BRIGHT GREEN
FLUORESCENCE WHEN EXPOSED
TO UV RAYS

3. HISTORY• Existed for more than one
hundred and sixty million years
in one species of jellyfish,
Aequorea Victoria.
• Was discovered by osamu
shimomura
• Cloned in 1994
• Awarded with chemistry nobel
prize

5. HOW IT GIVES GREEN LIGHT?

6. APPLICATIONS
• Natural Laser (2011)
• Fluorescence Microscopy
• Used to detect various diseases
• Widely being used in treating cancer
• Pathways of protein observation
• Even used for beautification projects

8. BRAINBOW!

9. IN-VIVO THERMOMETER

10. CANCER DETECTION

12. GLOWING SALAMANDER

13. HIV VIRULENCE
• Combination of GFP
with HIV on a T Cell
• How HIV proteins travel
down the nanotubes
from infected to non-
infected cells

14. GF PIXELS !
• By an Austrian
Artist and a
Biologist
• 4000 Petri Dishes
• Bacterial Cultures

16. FUCCI
FUCCI (FLUORESCENT, UBIQUITINATION-
BASED CELL CYCLE INDICATOR)
MODIFIED CELLS ARE YELLOW AT THE START
OF REPLICATION
SWITCH TO GREEN DURING S PHASE AND
RED DURING G1.

17. THINKING

18. DETECTING THE PORTIONING OF
EYE CELLS
FRUIT FLY (800 EYE UNITS)
BEETLES, MOSQUITOES AND
BEES (CLOSED SYSTEM)
ADDITION OF SPACEMAKER
PROTEIN

19. PARTS OF THE CEL

20. ROOT HAIRS

21. OVARIAN CELLS

22. REMARKABLE DISCOVERY