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Determine a ternary mixture formed from the proteins in the .pdf

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This document discusses forming a ternary mixture of 3 proteins that cannot be separated using cation exchange, anion exchange, hydrophobic interaction chromatography, or size exclusion chromatography. The proteins that could be selected are: bovine serum albumin, a-chymotrypsin, a-chymotrypsinogen, cytochrome C, ferritin, hemoglobin, a-lactalbumin, lactoperoxidase, myoglobin, and ribonuclease A.

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Determine a ternary mixture formed from the proteins in the table above such that none of the components can be separated out using any combination of the columns and conditions in the previous questions (cation exchange, anion exchange, HIC, or Superose 12 size exclusion) Select 3 proteins: bovine serum albumin a-chymotrypsin a-chymotrypsinogen cytochrome C ferritin hemoglobin a-lactalbumin lactoperoxidase myoglobin ribonuclease ACategorize the proteins below according to elution order:

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  • 1. Determine a ternary mixture formed from the proteins in the table above such that none of the components can be separated out using any combination of the columns and conditions in the previous questions (cation exchange, anion exchange, HIC, or Superose 12 size exclusion) Select 3 proteins: bovine serum albumin a-chymotrypsin a-chymotrypsinogen cytochrome C ferritin hemoglobin a-lactalbumin lactoperoxidase myoglobin ribonuclease ACategorize the proteins below according to elution order: