Use the images from the wild type cell shown below to answer.pdf

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The document discusses protein fusions used to mark organelles in wild type cells, including HDEL-dsRed which labels the endoplasmic reticulum and BFP-Pho8 which labels the vacuole. It asks the reader to (i) define the protein fusions and their localizations and functions, (ii) explain the significance of the position of alpha-synuclein inclusions relative to the HDEL-dsRed and BFP-Pho8 structures based on previous findings, and (iii) propose a model connecting an HDEL-dsRed structure to the ability of Nvj1 to suppress alpha-synuclein toxicity and discuss both HDEL-dsRed and BFP-Pho8

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Use the images from the wild type cell shown below to answer the following questions using your
understanding of our experiments this quarter and our reading of the literature. (i) Define the
protein fusions (i.e., what they encode for and relevant signal sequences) used to mark the
organelles in the images below. Explain their localizations and functions in the context of the cell
and/or the experiment. (ii) In the context of the findings from Yeger-Lotem et al 2009, explain the
significance of the position of the a-synuclein-GFP inclusions with respect to both the HDEL-
dsRed and BFP-Pho8. (iii) Propose and explain a model that connects the HDEL-dsRed structure
indicated by the arrow on the right to the ability of Nvj1 to suppress a-synuclein induced cell
toxicity. Connect the model to our observations this quarter and be sure to discuss both the HDEL-
dsRed and BFP-Pho8 structures.

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