Chitosan based bio-polymer play important role in the construction of tissue engineering scaffold.

1. Tissue Engineering of Nasal Septum Using Chitosan-
Based Material
by
Arunkumar rengaraj
(22141800)

2. Introduction
• Nasal septum divides the nasal chamber into two cavities.
• Conventional repair of nasal septum involves autologous rib or septum cartilage grafts and prosthetic devices.
• Its having limitations like tissue availability, and prosthesis related infection and extrusion.
• The objective of my work is to use chitosan based scaffold for nasal septum tissue engineering.
• Chitosan, a linear polysaccharide derived from crustacean shells and from many natural sources.
• It having advantages like polycationic nature, biodegradability, biocompatibility, mucoadhesiveness.
• In addition, it enhances epithelial permeability through the opening of tight junctions between epithelial cells.

3. Materials and methods
2 % solution of chitosan chitosan + 1 M acetic acid
chitosan-collagen membrane
Tissues (Human inferior turbinates ) were treated with 0.5% pronase+1:1 mixture (DMEM) +
Ham’s nutrient F12
centrifugation
suspended in DMEM/F12
1.5-mL cell suspension was seeded on collagen coated
Morphological Examination
• The ciliary beat was observed under inverted microscope (Leica, DMI 6000, Solms, Germany) with 400 magnification.
• Imaging of cilia movement was recorded at 240 frames per second with a high-speed complementary metal oxide
semiconductor (CMOS) camera (CMC1300; VDS Vossku hler GmbH, Osnabru ck, Germany).
spread on a glass plate
Evaporated at 50C
Mix 40 μg type I collagen

4. Reference
• Dong-Won Lee, Shawna A Shirley, Richard F Lockey and Shyam Mohapatra. Thiolated chitosan nanoparticles enhance anti-
inflammatory effects of intranasally delivered theophylline. Respiratory Research 2006; 7:112.
• Tsung-Wei Huang, Yi-Ho Young, Po-Wen Cheng, Yen-Hui Chan, Tai-Horng Young. Culture of Nasal Epithelial Cells Using
Chitosan-Based Membranes. The Laryngoscope 2009; 119:2066-2070.