Metabolic Pathways & Phyto-patho Interactions: Clues to Targets for Metabolic Engineered ‘Defense Bolstered Crops’ ?- Biswapriya Biswavas Misra, Ph.D., CCB@USM, 23rd August, 2012

1. Metabolic Pathways & Phyto-patho
Interactions: Clues to Targets for
Metabolic Engineered ‘Defense
Bolstered Crops’ ?
Biswapriya Biswavas Misra, Ph.D.
CCB
23rd August, 2012

2. CASE STUDY: 1

3. Model Fungal Genomes Studied:
1.Fusarium sps: Die backs, root rots etc.
2. Phytophthora sps: Leaf drop/ fall
3.Aspergillus sps: Collar rots
Model Plant Genomes Studied:
1.Arabidopsis thaliana [Model Plant]
2.Physcomitrella patens [Primitive Plant]
3.Chlamydomonas reinhardtii [Unicellular
Green Organism]

4. Generic ‘Terpenoid Metabolism’ in Living Organisms

5. Comparative Pathways Operative Across Various Kingdoms
Plants
Pathogens

6. Terpenoid Biosynthesis in …Arabidopsis thaliana
7 Essential
proteins.

7. Terpenoid Biosynthesis in …Physcomitrella patents
8 Operative
proteins.

8. Terpenoid Biosynthesis in …Chlamydomonas reinhardtii
8 [+ 1 OL]
Operative
Proteins

9. Terpenoid Biosynthesis in …Aspergillus species
8 [+2]
Operative
proteins.

10. Terpenoid Biosynthesis in …Fusarium species
6 Essential
proteins !!!

11. Terpenoid Biosynthesis in …Phytophthora species
5 [ +2 OL]
Operative
proteins ??

12. Carbon Fixation Routes in Plants & Pathogens
Total Number of Orthologs : 13 14 21 14 22 12 (reduced?)
Differential
Number of
proteins !

13. CASE STUDY: 2

14. Let us study 3 Phytopathogens:
1.Ganoderma sps.
2.Phytophthora sps.
3.Aspergillus sps.
[All are Pathogenic to Rubber Tree!]

15. Case 1: Phytophthora sps- GO Distributions

16. Case 2: Ganoderma sps- GO Distributions

17. Case 3: Aspergillus sps- GO Distributions

18. Summary- GO Distributions for 3 Model Pathogens

19. CASE STUDY: 3

20. Number of Arabidopsis thaliana Unique Genes Responding
To Stress [Biotic/ Abiotic]?
Insect
Bacterial/
Viral Fungal
Hormonal

21. Summarizing All Stress [Biotic/ Abiotic] Responsive
Genes…….into Pathways

22. …..reflected in Biosynthesis of Secondary Metabolism
Pathways…..

23. …..reflected in Starch and Sucrose Metabolic Pathways…..

24. Functions of genes Regulated in ABA Stress Response :
(as 2nd Level of GeneBins Ontology)

25. Functions of genes Regulated in Pseudomonad
[Pathogen] Challenge: (as 2nd Level of GeneBins Ontology)
NO
Difference ?
Pathogen vs
Abiotic Stress
Challenges in
terms of
Functions !

26. GO Distribution Network for ABA Responsive Genes in
Arabidopsis thaliana:

27. GO Distribution Network for Pseudomonad
[Pathogen] Challenged Arabidopsis thaliana:
Huge Complexity &
Networking of Cellular
Localization of
Proteins in Pathogenic
Stress ?

28. GO Distribution for Pseudomonad
[Pathogen] Challenged Arabidopsis thaliana:

29. GO Distribution for ABA Responsive Genes in
Arabidopsis thaliana:

30. Thank You
Note: Study is based solely on Publicly available Databases,
Platforms/ Softwares, Sequences, Codes, Programs and
Information and are exclusively GENERATED.
Appropriate sources are thanked.
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