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Pengaruh Acetobacter xylinum dan Ekstrak Kacang Hijau (Phaseolus radiatus
L.) terhadap Produksi Nata dari Substrat Limbah Cair Tahu

Nama : Dira Oktavia Yusvina
Nim : 092831
Kelas : VI D








Limbah cair tahu atau air kecutan (whey), air
limbah yang dapat memberikan nilai tambah
yang tinggi
Limbah berasal dari air rendaman kedelai, air
tirisan kedelai setelah perendaman, air hasil
pemisahan tahu dan air yang keluar saat
pengepresan berlangsung (Hastuti & Raharjo,
1983)
Nata, produk pangan berupa lapisan selulosa
sebagai hasil fermentasi bakteri pembentuk
nata, yaitu Acetobacter xylinum
Faktor-faktor pendukung pembentukan nata,
sumber N & kadar inokulum




Kacang hijau (Phaseolus radiatus L.) memiliki
kandungan gizi yang lengkap dibandingkan
kedelai dan kacang tanah (Rukmana, 1997)
Tujuan: mengetahui pengaruh kadar
inokulum Acetobacter xylinum dan
penambahan ekstrak biji kacang hijau
(Phaseolus radiatus L.) terhadap kualitas dan
kuantitas nata de soya dari medium air
kecutan (whey)




Variasi kadar inokulum: 50, 100, dan 150
ml/liter
Pengukuran:

◦ Parameter fisik nata, yaitu ketebalan dan
kekenyalan (Nugerahani et al., 1999)
◦ Parameter kimia nata: kadar air, kadar serat, dan
kadar N (Sudarmadji et al., 1989)
◦ Uji organoleptik: warna, rasa, dan aroma (Kartika &
Bambang, 1986)








Whey diendapkan (didiamkan), kemudian

disaring dengan kain kassa, lalu dipanaskan
sampai mendidih sambil diaduk
Tambahkan asam asetat glasial (10ml asam
asetat per liter whey) sampai pH 4 dan gula pasir
(80 gram gula per liter whey), disebut whey asam
bergula
Ekstrak kacang hijau dilarutkan dalam whey asam
bergula (masing-masing 0, 100, 200 dan 300
gram ekstrak kacang hijau dalam 100 ml whey)
Larutan ini dididihkan 15 menit, kemudian
dimasukkan ke dalam whey asam bergula,
larutan disebut medium nata.








Medium nata dimasukkan ke dalam wadahwadah fermentasi yang sudah disterilkan
Wadah ditutup dengan kertas bersih yang
telah di-oven pada suhu 95-100°C selama 2
jam dan diikat karet
Setelah dingin, medium nata ditambah starter
(tiap 1 liter medium ditambahkan masingmasing 10, 20 dan 30 ml starter)
Wadah berisi medium dan starter disimpan di
ruang fermentasi selama 10-15 hari sampai
terbentuk lapisan nata dengan ketebalan 1,52,0 cm (Anonim, 1998)














Gambar 1. Nata de soya dari berbagai

variasi konsentrasi sumber N dan
Inokulum

Keterangan:
A1 : starter 50 ml, kacang hijau 100 ml
A2 : starter 50 ml, kacang hijau 200 ml
A3 : starter 50 ml, kacang hijau 300 ml
A4 : starter 100 ml, kacang hijau 100 ml
A5 : starter 100 ml, kacang hijau 200 ml
A6 : starter 100 ml, kacang hijau 300 ml
A7 : starter 150 ml, kacang hijau 100 ml
A8 : starter 150 ml, kacang hijau 200 ml
A9 : starter 150 ml, kacang hijau 300 ml




Semakin tinggi kadar N dan inokulum yang
ditambahkan dalam medium, maka semakin
meningkat pula kadar air, kadar serat,
kekenyalan, dan kandungan N data nata yang
dihasilkan.
Penambahan N dapat menghasilkan tebal
rerata nata yang paling tinggi pada kadar
kacang hijau 200 g/L dan pemberian
inokulum yang menghasilkan rerata nata
yang lebih tebal terjadi pada kadar inokulum
100 ml/L.
Terima Kasih ….

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Biologi Terapan (Analisis Jurnal)

  • 1. Pengaruh Acetobacter xylinum dan Ekstrak Kacang Hijau (Phaseolus radiatus L.) terhadap Produksi Nata dari Substrat Limbah Cair Tahu Nama : Dira Oktavia Yusvina Nim : 092831 Kelas : VI D
  • 2.     Limbah cair tahu atau air kecutan (whey), air limbah yang dapat memberikan nilai tambah yang tinggi Limbah berasal dari air rendaman kedelai, air tirisan kedelai setelah perendaman, air hasil pemisahan tahu dan air yang keluar saat pengepresan berlangsung (Hastuti & Raharjo, 1983) Nata, produk pangan berupa lapisan selulosa sebagai hasil fermentasi bakteri pembentuk nata, yaitu Acetobacter xylinum Faktor-faktor pendukung pembentukan nata, sumber N & kadar inokulum
  • 3.   Kacang hijau (Phaseolus radiatus L.) memiliki kandungan gizi yang lengkap dibandingkan kedelai dan kacang tanah (Rukmana, 1997) Tujuan: mengetahui pengaruh kadar inokulum Acetobacter xylinum dan penambahan ekstrak biji kacang hijau (Phaseolus radiatus L.) terhadap kualitas dan kuantitas nata de soya dari medium air kecutan (whey)
  • 4.   Variasi kadar inokulum: 50, 100, dan 150 ml/liter Pengukuran: ◦ Parameter fisik nata, yaitu ketebalan dan kekenyalan (Nugerahani et al., 1999) ◦ Parameter kimia nata: kadar air, kadar serat, dan kadar N (Sudarmadji et al., 1989) ◦ Uji organoleptik: warna, rasa, dan aroma (Kartika & Bambang, 1986)
  • 5.     Whey diendapkan (didiamkan), kemudian disaring dengan kain kassa, lalu dipanaskan sampai mendidih sambil diaduk Tambahkan asam asetat glasial (10ml asam asetat per liter whey) sampai pH 4 dan gula pasir (80 gram gula per liter whey), disebut whey asam bergula Ekstrak kacang hijau dilarutkan dalam whey asam bergula (masing-masing 0, 100, 200 dan 300 gram ekstrak kacang hijau dalam 100 ml whey) Larutan ini dididihkan 15 menit, kemudian dimasukkan ke dalam whey asam bergula, larutan disebut medium nata.
  • 6.     Medium nata dimasukkan ke dalam wadahwadah fermentasi yang sudah disterilkan Wadah ditutup dengan kertas bersih yang telah di-oven pada suhu 95-100°C selama 2 jam dan diikat karet Setelah dingin, medium nata ditambah starter (tiap 1 liter medium ditambahkan masingmasing 10, 20 dan 30 ml starter) Wadah berisi medium dan starter disimpan di ruang fermentasi selama 10-15 hari sampai terbentuk lapisan nata dengan ketebalan 1,52,0 cm (Anonim, 1998)
  • 7.            Gambar 1. Nata de soya dari berbagai variasi konsentrasi sumber N dan Inokulum Keterangan: A1 : starter 50 ml, kacang hijau 100 ml A2 : starter 50 ml, kacang hijau 200 ml A3 : starter 50 ml, kacang hijau 300 ml A4 : starter 100 ml, kacang hijau 100 ml A5 : starter 100 ml, kacang hijau 200 ml A6 : starter 100 ml, kacang hijau 300 ml A7 : starter 150 ml, kacang hijau 100 ml A8 : starter 150 ml, kacang hijau 200 ml A9 : starter 150 ml, kacang hijau 300 ml
  • 8.   Semakin tinggi kadar N dan inokulum yang ditambahkan dalam medium, maka semakin meningkat pula kadar air, kadar serat, kekenyalan, dan kandungan N data nata yang dihasilkan. Penambahan N dapat menghasilkan tebal rerata nata yang paling tinggi pada kadar kacang hijau 200 g/L dan pemberian inokulum yang menghasilkan rerata nata yang lebih tebal terjadi pada kadar inokulum 100 ml/L.