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Cultivation of Medicinal Plants by Vegetative/Asexual Propagation
1. Cultivation Of Medicinal Plants by
Vegetative / Asexual propagation
PREPARED BY,
Ms. M.S. Divya Sree, M.Pharm,
Assistant Professor,
Department of Pharmacognosy,
Sree Vidyanikethan College of Pharmacy,
Tirupati, Andhra Pradesh.
2. Introduction
It is the process of multiplication in which a
portion of fragment of the plant body functions
as propagate and develops into a new individual.
Also known as Asexual propagation
There are different types of vegetative
propagations.
5. Artificial methods
Specific method or process is used on any vegetative part of the
plant for propagation.
Types
1) Cutting
2) Layering
3) Division
4) Grafting
5) Budding
6. 1) Cutting
A cutting is separated portion of root, stem or
leaf.
It is very common method employed for
artificial vegetative propagation.
Ex : Sugar cane, Roses, Citrus, Duranta,
Grapes, Cocoa, Bougainvillea.
8. I. Stem cutting
• Single node stem cutting – if internode is greater than
1.5 cm.
• Double node stem cutting – if internode is 1.5 cm.
• Multiple node stem cutting – if internode is very small.
• Soft wood cutting.
• Hard wood cutting.
26. Cell culture followed by
differentiation
• It is a collection of techniques used to
maintain or grow plant cells, tissues or organs
under sterile conditions on a nutrient culture
medium of known composition.
• It is widely used to produce clones of a plant
in a method known as Micropropagation.
27.
28. Micropropagation
• It is the practice of rapidly multiplying stock plant
material to produce a large number of progeny
plants, using modern plant tissue culture
methods.
• Can get high yield in short period of time.
• Micro quantities of Explant is enough to produce
many plants.
29. Micropropagation procedure
Selection of an explant from a ‘mother plant’ that
is healthy and vigorous.
Usually, apical buds are preferred as explants but
any other tissue can be used.
Establishment of this explant in a plant culture
medium.
30. (A medium supports growth and cell division.
Depending on the plant requirement, different
types of media are used for specific types of
plants.)
Multiplication - In this stage, the explants give
rise to a callus.
Differentiation.
Organogenesis.
31.
32.
33.
34.
35.
36.
37. Fermentation
Bio-fermentation technology makes it possible to
grow a number of fungi in large tanks.
In a matter of days, a large quantity can be
produced.
The technology has now become highly advanced
and is making previously rare herbs much more
accessible.
38. Many studies indicate that the chemical
nature of this biotechnology is almost
identical to that of the wild variety.
The most commercial products are produced
by this technology.
39. Advantages
• It is not as complex and requires far less energy than
sexual reproduction.
• It is a means of maintaining continuity.
• There is no need for much time or energy to produce
offspring.
• Cloned offspring are more likely to succeed in the same
stable areas as their parents.
40. Disadvantages
• Asexual reproduction does not have genetic diversity.
• The organism becomes less adapted to certain
environmental changes. The entire communities of the
genetically identical organisms might not survive such
changes.
• Asexual reproduction usually leads to struggle for
existence as well as overcrowding.