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S. cerevisiae




                + CO2 +
Malting
              3




                  5–
          7
Mashing
         Malt




         adjun
         t                 T
4             enz   malt
1. 45 C      30 –   60
       protease
2. 56 – 62 C        5 – 30
 -amylase
3. 57 – 72 C        14 – 45
 -amylase
4. 75 – 80 C        5 – 10
           wort
Wort

       hops
1.5 – 2
wort
     10 C
5


0–4C

            2

                &
bottom yeast
          S. carlsbergensis
C6H12O6           2
                   S. cerevisiae
               2C H OH + 2CO
                      5         2


                + glycerol acetic




                          ethanol +
Clostridium         butyric
acid
S. pastorianus

Hansenula anomala

Pediococcus
(18 – 24 Brix,   0.4 – 0.6
     %, pH 3.5 – 4.0)
red – 22 C, white 12 – 15
           C)
Winemaking
     Process
Grape picking   Fining
Destemming      Maturation
Crushing        Stabilization
Pressing        Aging
Settling        Blending
Fermentation    Filtration
Racking         Bottling
S. cerevisiae

 Film yeast oxidize alc -> acid


 Acetobacter alc -> acetic

 Lactic acid bacteria     L. brevis
Leuconostoc
mesenteroides         Leuc.
Dextranicum

Mucor, Penicillium,
Aspergillus
       cork taint
salami
probiotic

(106     cfu/g)
                             LAB
Streptococcus thermophilus
Lactobacillus bulgaricus
probiotics
  lactose
Streptococcus
thermophilus
Lactobacillus bulgaricus
                   40 –
49 C
Lactic acid bacteria Strep.
lactis

    curd
Vinegar
3
Acetic acid 5 %
                  cider




Acetobacter,
3
                  [alc] 7 –
              13 %
                   5

Starter 1/3
Generator




          4 – 10  (acetic
     acid ~ 11 %)
DAP, urea, peptone, yeast
extract
Submerged
Process




  ~ 36      starter
Yeast S. cerevisiae      -> alc
Gluconobacter, Acetobacter

T > 30 C MO
Film yeast
oxidize acetic
Dough, gluten
Koji =

Aspergillus oryzae
25 – 30 C, 3
30 C, 3
             [ ] 24 %
    2 – 12
Protease, amylase
A. oryzae
LAB      L. delbrueckii

B. subtilis, Ped. halophilus
S. rouxii
1
        2
       15 %     3

       =
15 %       5%
Single
cell protein (SCP)

           S. cerevisiae
SCP


60 – 70 %
Scenedesmus acutus,
Spirulina maxima
                CO2
Pseudomonas,
Hydrogenomonas,
Cellulomonas



                  %
Candida utilis




                 pH
Glutamate , MSG
Corynebacterium
Lysine
Dextran
     polysaccharide
 Leuconostoc
 mesenteroides
Xanthan
 Xanthomonas campestris
Homofermentative LAB
Lactobacillus delbruekii, L.
bulgaricus
citric acid

Aspergillus niger
+N
Amylase
 Rhizopus, Mucor, A.
 oryzae, A. niger, B. subtilis
Invertase
glu + fruc
 S. cerevisiae
Protease
 B. subtilis, A. oryzae

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