1. Welcome to the WP1 workshop! Increase efficiency of (marker assisted) breeding 29th March 2011

5. Presentation of the tasks and discussion

16. Selection of the progenies Let’s discuss few minutes about the progenies proposed …

17. Size ? New crosses ? Apple crosses in Bologna Year Code Genitori Totale 87 87403 Perleberg 3 x Florina 287 88 88419 Gelata x Red Chief 97 89 89401 Red Chief x Florina 99 89 89404 Royal Gala x Florina 200 89 89412 Royal Gala x B1R7T1 115 89 89414 Royal Gala x Gelata 147 89 89415 Royal Gala x Red Chief 193 91 91401 Belgolden x Braeburn 278 91 91405 Fuji houfu1 x Florina 89 91 91410 Stark Splendor x Royal Gala 241 95 95409 Durello di Forlì x Fiesta 209 96 96401 Discovery x Durello di Forlì 153 96 96405 Gold Chief x Durello di Forlì 170 96 96407 79410002 x Durello di Forlì 126 96 96409 GoldRush x 79410002 169 96 96412 Primiera x Abbondanza rossa 96 96 96415 Primiera x Pink Lady 93 96 96421 Fuji Aki-Fu 1 x Gold Chief 147 96 96424 Gold Chief x Pink Lady 169 96 96434 Gala x Golden 185 98 98427 GoldRush x PI 172612 99 98 98428 GoldRush x Russian 113 98 98430 GoldRush x White Angel 111 99 99401 Durello di Forlì x Golden Delicious 310 99 99402 Durello di Forlì x Granny Smith 201 99 99406 Harmonie x Murray 119 99 99411 Renetta Grigia di Torriana x Golden Delicious 155 99 99412 Renetta Grigia di Torriana x GoldRush 197 00 00403 GoldRush x Discovery 201 00 00404 GoldRush x Realka 142 00 00405 GoldRush x Amasya 94 03 03408 Ariwa x Pinova 146 05 05403 Golden x Murray 231 5382

18. 1) Parent 313 (Gala x Braeburn) x Fuji 2542 individuals in different planting seasons No selection on seedling level Trait is quality : appeal, texture, good balance sweet-acid and storability. Selection at better3fruit will be focused on appeal and taste. Approximately 5 % will be harvested. Keep ind. for FBO 2)Braeburn x Pri2482-100 400 individuals ; 2 different plantations? Trait: Vm resistance: AS-MGs Rvi5 Selection on seedling level with artificial infection: classes 0-1-2 retained. Susceptible selections not available. Selection at Better3fruit: all accessions will be harvested and described. Selection on quality: firmness, texture, storability, harvest time. No first selection on appeal 3) Parent 313 (Gala x Braeburn) x Alkmène 814 individuals ; 2 planting seasons Trait: Polygenic resistence (Alkmene) Selection on seedling level with artificial scab infection. Susceptible selections are not available 442 selections -planted in 2011- were planted in an unsprayed field. Field resistance observations will be possible. (257 plants budded in 2010 will be sprayed) Selection at Better3fruit: appeal, quality and storability: Less than 5 % will be harvested. Apple progenies proposed by B3F

19. 1) Pinova x INRA hyb Vf 1255 individuals but planted in 5 different sites ( ≈400 in 1 site + ≈ 200 in the 4 other sites) selection for scab on seedling level Segregating fruit traits: attractivenes, fruit quality, 2 nd flowering… 2) INRA hyb Vf x Choupette 915 (+184) individuals not selected for scab in greenhouse, to be planted in 1 site + 1438 selected for Vf (greenhouse test) to be planted in 5 sites ( 475 in the same site as above) Segregating fruit traits: attractivenes, fruit quality, 2 nd flowering… Polygenic (?) resistance Apple progenies proposed by Novadi Others existing or new crosses … (on request)

20. Peach progenies proposed by UMIL PROGENY GOAL CROSS YEAR SDLGS N° NOTES ON SEGREGATION BO 96016208 self MONI 02 359 F2 of Contender x Ambra : brown rot on fruit, glabrous skin, fruit quality; genotyped SOLEADA * GLOHAVEN MONI 02 115 Brown rot on fruit Soleada * MAYCREST QUAL 02 134 Brown rot on fruit, ripening date BIG TOP * BIG BANG BGTP 04 93 Fruit quality, ripening date and flesh texture BO 93030045 * DIAMOND BRIGHT BGTP 04 164 Fruit quality, ripening date and flesh texture BO 93041003 self HABI 04 110 Growth habit and fruit size and quality BO 96016097 * MAY GLO MONI 04 178 Brown rot on fruit ELEGANT LADY self MAPPA 04 95 Glabrous skin and ripening date GLOHAVEN * GF 677 MAPPA 04 92 Back cross from almond x peach hybrid ; fruit size and quality, ripening date, tree vigour GUERRIERA * BO 96003028 BGTP 04 125 Fruit quality and ripening date, flesh texture MAX 10 * BO 96003028 BGTP 04 246 Fruit quality and ripening date, flesh texture RED MOON * CONTENDER MONI 04 117 Brown rot on fruit, fruit quality and ripening date S 6699 * BORDO' AFIDI 04 309 Green aphid resistance, fruit quality and ripening date S 6699 * DIAMOND BRIGHT AFIDI 04 256 Green aphid resistance, fruit quality and ripening date STARK RED GOLD * BO 96003028 BGTP 04 98 Fruit quality and ripening date, flesh texture BO 92038046 self MAPPA 05 97 Brown rot on fruit BO 92038071 * SUPEACHSIX MONI 05 112 Brown rot on fruit D 41-62 self STHA 05 151 All seedlings stony hard and same ripening date DIAMOND RAY * BO 89015029 QUAL 05 165 Fruit quality and ripening date NADIA (BO 82015079) * BO 00025004 MONI 05 115 Brown rot on fruit, fruit quality and ripening date ORION * DIAMOND BRIGHT QUAL 05 136 Fruit quality and ripening date SOLEADA * S 6699 AFIDI 05 165 Green aphid resistance, fruit quality and ripening date BO 02028017 self MAPPA 06 110 F2 of MORSIANI 90 x EARLY SILVER; glabrous skin, fruit quality and ripening date BO 92050007 self MAPPA 06 91 F2 of NJ WEEPING (PI 91459) x BOUNTY; growth habit, flesh texture (melting-non melting), fruit quality and ripening date BO 92050010 self MAPPA 06 128 F2 of NJ WEEPING (PI 91459) x BOUNTY; growth habit, flesh texture (melting-non melting), fruit quality and ripening date HONEY GLO * BO 96028059 BGTP 06 1060 Fruit quality, ripening date and flesh texture ALITOP * RUBY RICH BGTP 07 150 Fruit quality, ripening date and flesh texture ALIX * RED MOON QUAL 07 103 Fruit quality, ripening date and flesh texture BO 88008037 * FRANCESCA MONI 07 211 Fruit quality and brown rot on fruit BO 92038046 self MAPPA 07 126 F2 of Contender x Elegant Lady ; Fruit quality and brown rot on fruit BO 92038071 self MAPPA 07 280 F2 of Contender x Elegant Lady; Fruit quality and brown rot on fruit BO 92038140 self MAPPA 07 250 F2 of Contender x Elegant Ladys; Fruit quality and brown rot on fruit

21. Peach progenies proposed by UMIL (following) PROGENY GOAL CROSS YEAR SDLGS N° NOTES ON SEGREGATION BO 96013007 self BGTP 07 209 F2 of Bolero x Rich Lady ; Flesh texture and fruit quality BO 96027023 self MAPPA 07 107 F2 of SPLENDOR x TOP LADY s; fruit quality, flesh colour and quality DIALONA * RITA STAR QUAL 07 509 Fruit quality DIAMOND BRIGHT * ALICE QUAL 07 162 Fruit quality DORIS * BO 88008091 QUAL 07 494 Fruit quality GUERRIERA * HONEY ROYAL BGTP 07 902 Fruit quality, flesh texture and ripening date HONEY GLO * ALEXA BGTP 07 715 Fruit quality, flesh texture and ripening date HONEY GLO * BORDO' BGTP 07 1256 Fruit quality, flesh texture and ripening date HONEY GLO * DIAMOND RAY BGTP 07 989 Fruit quality, flesh texture and ripening date MARIA DORATA * HONEY ROYAL PC 07 324 Fruit quality ORION * SD 75 PPV 07 358 Back cross from peach x Prunus davidiana; Flesh colour and fruit quality SWEET FIRE * GARCICA BGTP 07 1051 Fruit quality and flesh texture SWEET LADY * HONEY ROYAL BGTP 07 269 Fruit quality and flesh texture SWEET SILVER * GARCICA BGTP 07 451 Fruit quality and flesh texture TARDIBELLE * SPRINGBELLE QUAL 07 152 Ripening date and fruit quality VISTA RICH * BO 02009066 BGTP 07 133 Flesh texture, fruit quality and brown rot on fruit NADIA (BO 82015079) * BO 92038173 MONI 08 130 Fruit quality and brown rot on fruit CONTENDER * ELEGANT LADY MONI 92 200 Brown rot on fruit, ripening date and fruit quality; genotyped BOLERO * ORO A OIDIO 96 183 Powdery mildew, ripening date and flesh texture; genotyped

22. WP1 - T1.4 Genotyping pipeline 29th March 2011

27. WP1 - T1.5 Specification list of the breeding interface 29th March 2011

32. Action plan WP1 first 12 months