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[object Object]
INTRODUCTION
 
[object Object],[object Object],[object Object],[object Object]
FREE RADICALES ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
TYPES OF FREE RADICALS  ,[object Object],[object Object],[object Object],[object Object],[object Object]
SUPEROXIDE RADICAL ,[object Object],[object Object],[object Object],[object Object],[object Object]
HYDROXYL RADICAL ,[object Object],[object Object],[object Object]
[object Object],[object Object],[object Object],[object Object]
 
BENEFICIAL ACTIVITIES ,[object Object],[object Object],[object Object],[object Object]
DELETERIOUS   ACTIVITY  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Cancer and oxidative stress…….. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Arthritis and oxidative stress …. ,[object Object],[object Object],[object Object],[object Object]
[object Object],[object Object]
[object Object],[object Object],[object Object]
Atherosclerosis and Oxidative stress…. ,[object Object],[object Object],[object Object],[object Object],[object Object]
Diabetes and Oxidative Stress… ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Neurodegenerative disorders  ,[object Object],[object Object]
Erectile dysfunction and oxidative stress……… ,[object Object],[object Object],[object Object],[object Object],[object Object]
What are Antioxidants
Antioxidants may be classified as………. ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Mechanism of Antioxidants ,[object Object],[object Object],[object Object],[object Object]
 
 
Need of screening for antioxidants
 
CRITERIA FOR SELECTION OF  SCREENING MODEL  ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Target Oxidized Target Oxidized antioxidant Antioxidant 1. Competitive Reactive species  Reduced reactive species
Oxidized antioxidant Antioxidant 2. Non-competitive Reactive species  Reduced reactive species
[object Object]
Hydroxyl radical scavenging activity [OH - ] ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Before addition of antioxidant FeCl3 H2O2 EDTA OH Deoxyribose Chromogen TBA
After addition of antioxidant FeCl3 H2O2 EDTA OH Deoxyribose Chromogen (532 nm) TBA
Superoxide radical anion (O2 .- ) scavenging assay ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Before addition of antioxidants agent O2 - Nitro  blue terazolium reduction Formazan (560 nm)
After addition of antioxidants agent Nitro  blue terazolium Reduction Formazan
[object Object],[object Object],[object Object],[object Object]
Nitric oxide scavenging activity [NO . ]………. ,[object Object],[object Object],[object Object]
DPPH .  Scavenging assay  ,[object Object],[object Object],[object Object],[object Object]
Peroxyl radicals {ROO . }  scavenging activity ,[object Object],[object Object],[object Object],[object Object],[object Object]
ORAC ,[object Object],[object Object],[object Object],[object Object],[object Object]
Ferric reducing antioxidant power (FRAP) assay ,[object Object],[object Object],[object Object]
Folin-ciocalteu capacity ,[object Object],[object Object],[object Object]
Other Antioxidant Models ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
List of Antioxidant plants from “RASAYANA” Acorus calamus Aloe vera Asparagus racemosus  Azardirachta indiaca Bacopa monnieri Desmodium gangeticum Phyllanthus emblica Terminalia chebula Tinospora cordifolia  Withania somnifera
Other Ayurvedic plants Piper betel Santalum album Piper nigrum Swertia chirata Plumbago zeylanica Andrographis paniculata Curculigo Orchioides   Glycyrrhiza glabra Hygrophila auriculata Hemidesmus indicus Cassia fistula Punica granatum Mangifera indica Shorea robusta Curcuma longa Cassia sophera Emblica officinalis Calicarpa macrophyla Momordica charantia Allium sativum
Ayurvedic plants-----unfolded antioxidant potential Pluchea lanceolata Prunus cerasoides Salix caprea Hyoscymus niger Litea glutinosa Semecarpus anacardium Salmalia malabarica
These plants   can be acts as best antioxidants. Steppe plants Dessert plants Alpine plants Mountain plants
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
DIETARY ANTIOXIDANT
 
 
Conclusion ,[object Object]
[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
[object Object]
[object Object]
 

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D. H. Nagore Antioxidant Ppt

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  • 26. Need of screening for antioxidants
  • 27.  
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  • 29. Target Oxidized Target Oxidized antioxidant Antioxidant 1. Competitive Reactive species Reduced reactive species
  • 30. Oxidized antioxidant Antioxidant 2. Non-competitive Reactive species Reduced reactive species
  • 31.
  • 32.
  • 33. Before addition of antioxidant FeCl3 H2O2 EDTA OH Deoxyribose Chromogen TBA
  • 34. After addition of antioxidant FeCl3 H2O2 EDTA OH Deoxyribose Chromogen (532 nm) TBA
  • 35.
  • 36. Before addition of antioxidants agent O2 - Nitro blue terazolium reduction Formazan (560 nm)
  • 37. After addition of antioxidants agent Nitro blue terazolium Reduction Formazan
  • 38.
  • 39.
  • 40.
  • 41.
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  • 44.
  • 45.
  • 46. List of Antioxidant plants from “RASAYANA” Acorus calamus Aloe vera Asparagus racemosus Azardirachta indiaca Bacopa monnieri Desmodium gangeticum Phyllanthus emblica Terminalia chebula Tinospora cordifolia Withania somnifera
  • 47. Other Ayurvedic plants Piper betel Santalum album Piper nigrum Swertia chirata Plumbago zeylanica Andrographis paniculata Curculigo Orchioides Glycyrrhiza glabra Hygrophila auriculata Hemidesmus indicus Cassia fistula Punica granatum Mangifera indica Shorea robusta Curcuma longa Cassia sophera Emblica officinalis Calicarpa macrophyla Momordica charantia Allium sativum
  • 48. Ayurvedic plants-----unfolded antioxidant potential Pluchea lanceolata Prunus cerasoides Salix caprea Hyoscymus niger Litea glutinosa Semecarpus anacardium Salmalia malabarica
  • 49. These plants can be acts as best antioxidants. Steppe plants Dessert plants Alpine plants Mountain plants
  • 50.
  • 52.  
  • 53.  
  • 54.
  • 55.
  • 56.
  • 57.
  • 58.