Recomendados
Más contenido relacionado
Similar a Lecture 4 Exercises for herpes 1&2.pptx
Similar a Lecture 4 Exercises for herpes 1&2.pptx (20)
Más de hakim hassan
Más de hakim hassan (20)
Último
Último (20)
Lecture 4 Exercises for herpes 1&2.pptx
- 1. Saleh Bahaj Prof. of Medical Microbiology/Immunology Herpesviridae Lecture 4
- 3. Objectives By the end of this lecture the students will be able to:- I) Definition II) Pathogenesis III) Diseases IV) Lab. Diagnosis V) Treatment VI) Prevention Herpes simplex virus-1 Herpes simplex virus-2 Objectives Introduction
- 5. DNA All DNA viruses dsDNA EXCEPT Parvovirus B19 (ssDNA)
- 6. DNA All DNA viruses Linear EXCEPT Papovavirus (circular)
- 8. DNA All DNA viruses Replicate in nucleus (inclusion bodies present intranuclear ) EXCEPT Poxvirus in cytoplasm (intracytoplasmic)
- 9. Herpes Family 1) Herpes simplex Viruses HSV(1&2) 2) Varicella-Zoster virus (VZV) 3) Cytomegalovirus (CMV) 4) Epstein-Barr virus (EBV) 5)Human herpes virus HHV(6&7) 7)Human herpes virus (HHV8)
- 10. Herpes Family Herpein = Creep
- 12. Characteristic of Herpesviridae All members are dsDNA with enveloped dsDNA 1
- 14. Characteristic of Herpesviridae HSV-1 HSV-2 VSV EBV CMV HHV-6 HHV-7 HHV-8 3 Similar morphology
- 15. Characteristic of Herpesviridae 4 Latency
- 16. Characteristic of Herpesviridae 5 linked with tumor
- 17. Characteristic of Herpesviridae 6 Response to anti-viral drugs
- 19. 1) Herpes simplex virus type-1 (HSV-1) Herpesviridae
- 20. I) Definition of (HSV-1) HSV-1 HSV-2 Belt
- 21. Herpes labialis
- 22. II) Pathogenesis oh HSV-1
- 23. Herpes labialis
- 26. III) Diseases
- 32. III) Diseases Herpes labialis Herpetic whitlow Gingivostomatitis Keratoconjunctivitis Encephalitis Aseptic meningitis
- 33. 1) Herpes simplex virus type-1 (HSV-1) IV) Lab. diagnosis
- 35. IV) Lab. diagnosis Specimen Vesicular fluid Scraping of ulcer Corneal scrapping CSF
- 36. 1) Direct virus demonstration A- Tzanck smear (multinucleated giant cells) Giemsa stain (Light Microscopy) Intranuclear inclusion bodies Scraping of ulcer
- 37. 1) Direct virus demonstration C) Detection of viral antigen by DIF From specimen Anti- HSV-1
- 38. 1) Direct virus demonstration D) Detection of Nucleic acid by PCR
- 39. 2) Viral Isolation A) Isolation the virus in tissue culture (monkey kidney) by Immunofluoresence
- 40. 2) Viral Isolation A) Isolation the virus in tissue culture (monkey kidney) by Immunofluoresence CPE
- 41. 3) Serological tests A) Detection of IgM/IgG ELISA Serum Encephalitis
- 42. 1) Herpes simplex virus type-1 (HSV-1) V) Treatment
- 44. V) Treatment Acyclovir (Prevent replication) Effect on viral DNA polymerase enzyme
- 45. 1) Herpes simplex virus type-1 (HSV-1) VI) Prevention
- 46. VI) Prevention Avoid contact With herpetic lesion
- 49. Q1 Herpesviridea family is characterized by Monocyte 2-3 days Latency Reactivation Chronic infection Response to viral therapy
- 50. Q2 Monocyte 2-3 days HSV-1 cause the following diseases Monocyte 2-3 days Gingivostomatitis Herpes labialis Herpetic whitlow Neonatal herpes
- 51. Q3 Monocyte 2-3 days HSV-1 remain latent Monocyte 2-3 days Monocyte 2-3 days Sacral ganglia Trigeminal ganglia Lymphoid cells B cells
- 52. Q4 Monocyte 2-3 days During latency, Herpes simplex virus-1 causes Genital herpes Herpetic whitlow Keratoconjunctivitis No clinical manifestations
- 53. 2) Herpes simplex virus type-2 (HSV-2) I) Definition II) Pathogenesis III) Diseases IV) Lab. Diagnosis V) Treatment VI) Prevention
- 54. I) Definition of HSV-2 ds DNA enveloped icosahedral virus attacks the mucous membranes of the genital tract.
- 55. 2) Herpes simplex virus type-2 (HSV-2) II) Pathogenesis
- 56. II) Pathogenesis of HSV-2 Sexual transmission H H O
- 57. II) Pathogenesis of HSV-2 From mouth to genital HSV-1
- 58. II) Pathogenesis of HSV-2 Vertical transmission
- 59. II) Pathogenesis of HSV-2 Asymptomatic Shedding
- 60. II) Pathogenesis of HSV-2 Sexual transmission Multiple at site of entry Formation of vesicles (ulcerate) Sacral ganglia
- 61. 2) Herpes simplex virus type-2 (HSV-2) III) Diseases
- 62. III) Diseases 1- Genital herpes Vesicular Lesions On glans (end of the penis), shaft of the penis.
- 64. III) Diseases 2- Neonatal herpes Asymptomatic Generalized disease Encephalitis
- 65. III) Diseases 2- Neonatal herpes Birth canal
- 67. Herpes simplex virus type-2 (HSV-2) IV) Lab. diagnosis
- 68. IV) Lab. diagnosis Discus with your team How can you diagnosis of HSV-2?
- 70. IV) Lab. diagnosis Specimen Vesicular fluid Scraping of ulcer
- 71. IV) Lab. diagnosis A- Tzanck smear (multinucleated giant cells) Giemsa stain (Light Microscopy) Intranuclear inclusion bodies
- 72. IV) Lab. diagnosis B) Detection of viral antigen by DIF From specimen Anti- HSV-2
- 73. IV) Lab. diagnosis C) Detection of Nucleic acid by PCR
- 74. IV) Lab. diagnosis D) Isolation the virus in tissue culture (monkey kidney) by Immunofluoresence
- 75. IV) Lab. diagnosis D) Isolation the virus in tissue culture (monkey kidney) by Immunofluoresence CPE
- 76. Herpes simplex virus type-2 (HSV-2) V) Treatment
- 78. V) Treatment Acyclovir (Prevent replication) Effect on viral DNA polymerase enzyme
- 80. 2) Herpes simplex virus type-2 (HSV-2) VI) Prevention
- 82. Exercise-1 HSV-2 HSV-1 Item 2- Mode of transmission 1- Target 3- Diseases Lips, nose & eyes Genital tract Contact Sexual transmission Herpes labials Herpetic whitlow Gingivostomatitis Keratoconjunctivitis Genital herps Neonatal herpes
- 83. Exercise-1 HSV-2 HSV-1 Item 5- Specimen for diagnosis 4- Latency Trigeminal ganglia Sacral ganglia Skin lesion
- 84. V) Test yourself
- 85. Q1 Monocyte 2-3 days HSV-2 cause the following diseases Monocyte 2-3 days Genital herpes Herpes labialis Herpetic whitlow Neonatal herpes
- 86. Q2 Monocyte 2-3 days HSV-2 remain latent Monocyte 2-3 days Monocyte 2-3 days Sacral ganglia Trigeminal ganglia Lymphoid cells B cells
- 87. Q3 Monocyte 2-3 days Infection with HSV-2 occurs by the following modes Sexual contact Transplacental Through birth canal Reactivation of latent virus
- 88. Q4 Monocyte 2-3 days During latency, Herpes simplex virus-2 causes Genital herpes Herpetic whitlow Keratoconjunctivitis No clinical manifestations
- 90. 3) Varicella-Zoster virus I) Definition II) Pathogenesis III) Diseases IV) Lab. Diagnosis V) Treatment VI) Prevention
- 91. I) Def. of Varicella-Zoster virus ds DNA, enveloped, icosahedral virus leading to generalized rash
- 93. II) Pathogenesis of Varicella-Zoster virus Varicella (Chicken pox) Zoster (Shingles) Primary infection Reactivation infection
- 94. II) Pathogenesis of Varicella-Zoster virus Droplets Direct contact with vesicle Trancplacental
- 95. II) Pathogenesis of Varicella-Zoster virus Respiratory mucosa
- 96. II) Pathogenesis of Varicella-Zoster virus Replication in regional nodes
- 97. II) Pathogenesis of Varicella-Zoster virus Blood (1st viremia)
- 98. II) Pathogenesis of Varicella-Zoster virus Into liver & spleen
- 99. II) Pathogenesis of Varicella-Zoster virus Blood (2nd Vireamia)
- 100. II) Pathogenesis of Varicella-Zoster virus Into respiratory mucosa Into mononuclear cells
- 101. II) Pathogenesis of Varicella-Zoster virus Transport to skin & mucous membrane
- 102. II) Pathogenesis of Varicella-Zoster virus Replication in epidermal cells
- 103. II) Pathogenesis of Varicella-Zoster virus Skin rash Virus in dorsal root ganglia Lesion Umbilicate Polymorphic (macule, papule, pustule , ulceration
- 104. Centripetal rash (in smallpox centrifugal واليدين الرجلين األطراف في distribution) • Superficial Do not reach basal cell layer (Healing without disfigurement) • Umbilicated lesion • Polymorphic (papule, pustules, vesicle at the same time in a same
- 105. II) Pathogenesis of Varicella-Zoster virus Skin rash Trunk Face limbs
- 106. II) Pathogenesis of Varicella-Zoster virus The rash start as macular
- 107. II) Pathogenesis of Varicella-Zoster virus Rapidly evolves papule
- 108. II) Pathogenesis of Varicella-Zoster virus vesicle (dew drops)
- 109. II) Pathogenesis of Varicella-Zoster virus Pustule & Crust
- 110. II) Pathogenesis of Varicella-Zoster virus All stages may be seen at one time
- 111. II) Pathogenesis of Varicella-Zoster virus Droplets Or contact Respiratory mucosa regional nodes 2nd viremia Into liver & spleen 1ry viremia Into Mononuclear cells I.P 10-21 days
- 112. II) Pathogenesis of Varicella-Zoster virus Into mononuclear cells Transport to skin & mucous membrane dorsal root ganglia Skin rash Replication in epidermal cells I.P 10-21 days
- 113. II) Pathogenesis of Varicella-Zoster virus Pre-eruptive stage Fever (firstly) Eruptive stage After 1-2 days Malaise & headache Macule, papule, vesicle & Pustule (Crusts) Trunk Face Limbs
- 115. Congenital varicella syndrome 1st & 2nd trimester the fetus is at increased risk of developing congenital varicella syndrome
- 116. Congenital varicella syndrome RARE Mental retardation Limb abnormalities Eye abnormalities
- 119. Zoster
- 121. Zoster Singles = inflammation often extends around the middle of the body
- 122. Zoster Reactivation of the virus
- 123. Zoster A belt roses from hell
- 126. 1- postherpetic neuralgia Constant pain at the site (Stabbing pain) for 1 month or year in 50% patients after the rash heal.
- 127. 2- Ophthalmic zoster
- 128. 3) Varicella-Zoster virus III) Lab. diagnosis
- 130. III) Lab. diagnosis 1) Direct A)Virus demonstration B) Virus Isolation 2) Indirect A)Serological tests (Serum)
- 131. 1) Direct virus demonstration A- Tzanck smear (multinucleated giant cells) Giemsa stain (Light Microscopy)
- 132. 1) Direct virus demonstration Intranuclear inclusion bodies (Light Microscopy)
- 133. 1) Direct virus demonstration B) Detection virus particles by EM!!!!!
- 134. 1) Direct virus demonstration C) Detection of viral antigen by DIF From specimen
- 135. 1) Direct virus demonstration D) Detection of Nucleic acid by PCR
- 136. 2) Viral Isolation A- Isolation the virus in tissue culture (monkey kidney) by Immunofluoresence
- 137. 2) Viral Isolation A- Isolation the virus in tissue culture (monkey kidney) by Immunofluoresence CPE
- 138. 3) Serological tests A) Detection of IgM Serum Recent Infection
- 139. 3) Serological tests A) Detection of Antibodies ELISA!!!! Serum
- 140. 3) Serological tests 4- Detection of rising titre of IgG by ELISA 1/80 2nd sample after 7 days 1/320 Serum
- 141. Exercise 2 Discuss with your team What is the clinical spot diagnosis of Varicella (Chickenpox)
- 142. Answer of Exercise 2 Fever Rash (Starting in trunk) Then Macule Papule Vesicle Pustule Present at the same time 1 2 3
- 144. IV) Treatment Acyclovir Valacyclovir Both varicella or zoster (Prevent replication)
- 145. IV) Treatment Avoid Aspirin Reye’s syndrome
- 147. V) Prevention Varivax (1995) Zostavax (2006) Shingrix (2017) Chickenpox Zoster
- 148. V) Prevention A living attenuated varicella vaccine 1995 (Varivax) 2 doses
- 149. V) Prevention Zostavax Attenuated Single dose Older age (60y.)
- 150. V) Prevention Zostavax Not used for children Not substitute for Varivax
- 152. V) Prevention VZIG
- 153. V) Test yourself
- 154. Q1 Monocyte 2-3 days The spot diagnosis of varicella (Chickenpox) Centripetal rash All types of rash are present Umbilicated lesion Heal with scar
- 155. Q2 The mode transmission of Zoster Droplets Contact Endogenous
- 156. Q3 Monocyte 2-3 days The mode of transmission of varicella (chickenpox) Droplets Contact Endogenous
- 157. Q4 Monocyte 2-3 days The varicella –zoster virus latent on Trigeminal ganglia Sacral Ganglia B cells No clinical manifestations
- 159. II) Pathogenesis of HSV-2 Multiple at site of entry (mucous membrane)
- 160. II) Pathogenesis of HSV-2 Formation of vesicles (ulcerate)
- 161. II) Pathogenesis of HSV-2 Sacral ganglia Reactivation (recurrent lesion)