POST-LAB QUESTIONS Fructose is a ketose- Ketones usually do not give a.docx

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1. Fructose is a ketose sugar but gives a positive reaction with Fehling's reagent because the base in the reagent shifts the double bond to convert the ketone group into an aldehyde, allowing it to react. 2. The iodine test is used to indicate the completion of starch hydrolysis by acid. 3. Acid hydrolysis of starch is faster than enzymatic hydrolysis. 4. Amylose gives a positive iodine test and negative Fehling's test due to its linear structure without an aldehyde or ketone group that could react with Fehling's reagent.

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POST-LAB QUESTIONS Fructose is a ketose. Ketones usually do not give a positive reaction
with Fehling's reagent. Why is fructose oxidized by Fehling's reagent? (Consult your textbook)
2. What chemical test is performed to indicate the completion of the hydrolysis of starch by acid?
3. Which hydrolysis of starch is faster: the acid or the enzyme catalyzed reaction? 4. In your
textbook the structure of amylose is depicted. Amylose gives a positive ?ndine Ist and a negative
tt wt Fehling's reagent. On the basis of the structure of amylose explain the results of the two
tests.
Solution
Ans 1 :
Fehling's test is given positive only by aldehydes , so it becomes a differentiating test between
aldehydes and ketones.
But fructose being a ketose sugar also gives positive fehling's test because the base present in
the reagent shifts the double bonded carbon over the terminal position , due to which keto group
is converted into aldehydic group now. So the sugar then gives a positive test to the reagent.

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