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Similar a Role of Type I Secretion in Pierce's Disease - Dean Gabriel - Pierce's Disease Conference 2008
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Role of Type I Secretion in Pierce's Disease - Dean Gabriel - Pierce's Disease Conference 2008
- 1. Role of Type I Secretion in Pierce’s Disease Project Leader: Dean W. Gabriel Plant Pathology Department University of Florida Gainesville, FL 32611 Cooperator: Donald Hopkins Mid Florida Res. Edu. Center University of Florida Apopka, FL Postdoctoral Associate : Shujian Zhang Plant Pathology Department University of Florida Gainesville, FL 32611 Funding: U.C. Davis subaward from USDA Cooperative Agreement SA6477 Research Associate : Stephanie Reddy Plant Pathology Department University of Florida Gainesville, FL 32611
- 4. O.M. Peptidoglycan I.M. Periplasm Secretion of proteins produced by the bacterium Efflux of toxic substances entering the bacterium ATP ADP Type I secretion machine has 2 roles
- 5. Type I secretion (PD1964) Figure from Koronakis et al. 2004 Ann. Rev. Biochem. 73:467 (PD0783) (PD1404) AcrF AcrD Only one (1) TolC (PD1964) in X. fastidiosa
- 6. Figure from Koronakis et al. 2004 Ann. Rev. Biochem. 73:467 Type I secretion: PD1964 (TolC) is common component. (PD1964) (PD1413) (PD1412) Two potential toxin secretion systems: HlyD (PD1413)/ HlyB (PD1412) & CvaA (PD0496)/ CvaB (PD0499)
- 7. Reddy et al. 2007. MPMI 20:403-10. PD1964 ( tolC ) 1019 bp 3033 bp A D C B Marker exchange of tolC in Temecula nptII 4033 bp F E D A B C 2019 bp 2000 bp 1454 bp 1kb wt M1 M2 wt M1 M2 wt M1 M2 wt M1 M2 wt M1 M2 100 bp A & D B & C A & E F & D RST31&33 pJR6.1 nptII PD1964 ( tolC ) 23 9 Wt M1
- 8. PD-T Wild Type 64 dpi Type I secretion/efflux tolC ¯ tolC :: nptII mutants are completely nonpathogenic... Reddy et al. 2007. MPMI 20:403-10.
- 10. PD-T Wild Type 64 dpi Type I MDR efflux acrD ¯
- 11. PD-T Wild Type 64 dpi Type I hemolysin secretion hlyBD ¯
- 12. PD-T Wild Type 64 dpi Type I colicin secretion cvaA ¯
- 13. PD-T Wild Type 64 dpi Type I colicin secretion cvaB ¯
- 14. # total leaves produced = # asymptomatic (healthy) leaves, # symptomatic (diseased) leaves, # bare petioles, # bare nodes # total symptomatic leaves = # symptomatic leaves, # bare petioles, # bare nodes
- 15. MDR efflux: acrD, acrF, tolC Type I effector secretion: hlyB, hlyD, cvaA, cvaB, tolC
- 16. TolC is externally exposed and might be blocked by chemicals or engineered plants. (PD1964)