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Plant GeneticsPlant Genetics
JJA Obico, Department of BiologyJJA Obico, Department of Biology
University of the Philippines Manila
Study of heredity and hereditary variationS udy o d y a d d a y a a o
Genes
- hereditary units
- Segments of DNA
Chromosomes
- DNA + proteins
GeneticsGeneticsGeneticsGenetics
A. Interphasep as
1. Gap 1 or G1 phase
2. Synthesis phase or S phase
3. G2 phase
B. M-phase (Mitosis or Meiosis)
Cell CycleCell CycleCell CycleCell Cycle
1. G1
h th ll d◦ where the cell spends
most of its functional
life
◦ Performs metabolic
acitivities
2. S-phase
◦ DNA molecules are
copied or replicatedcopied or replicated
◦ from single
stranded DNA in G1
phase to double
stranded DNAstranded DNA
3. G2
◦ preparing for the
actual division events
InterphaseInterphase
(much protein
synthesis- tubulin)
InterphaseInterphase
MeiosisMeiosisMeiosisMeiosis
Asexual or Sexual Reproduction?p
1. Crossing over
2. Independent assortment of chromosomes
3. Random fertilization
SOURCES OF VARIATIONSOURCES OF VARIATIONSOURCES OF VARIATIONSOURCES OF VARIATION
Crossing overCrossing overCrossing overCrossing over
Independent assortment of chromosomesIndependent assortment of chromosomesIndependent assortment of chromosomesIndependent assortment of chromosomes
2n
223 The no. of possible combination
of maternal and paternal chromosomes in
the gametes
Random fertilization of an ovumRandom fertilization of an ovum
by a spermby a spermby a spermby a sperm
True-breeding plantsu b d g p a s
Two varities
◦ Purple flower
◦ White flower
Hybridization
M h b idMonohybrid cross
Whatever the factor that caused thea a o a aus d
purple color was more powerful than
the factor for white color
Mendel said that the yellow factor
"d i t d" th f t"dominated" the green factor.
1. Alternative version of genes account fora s o o g s a ou o
variations in inherited character
Mendel’s findingsMendel’s findingsMendel s findingsMendel s findings
2. For each character, an organism inheritso a a a , a o ga s s
2 alleles: one from each parent
Homozygous– identical alleles
Heterozygous– different alleles
3. Dominant allele – fully expressed3 o a a u y p ss d
Recessive allele – masked
Dominant- uppercase
Recessive – lowercase
P- purple; p- white
4. Two alleles for each character segregateo a s o a a a s g ga
during gamete production – LAW of
SEGREGATION
AN ovum or sperm get only ONE of alleles
f ll thof all the genes
PUNNETT squareU squa
- A tool for predicting the results of a
genetic cross
P P
p Pp Pp
p Pp Pp
What will be the physicalWhat will be the physical
appearance?appearance?appearance?appearance?
Self pollinate Pp individualsS po a p d dua s
P p
P PP Pp
P Pp pp
Pp, PPPp, PP-- same phenotype but different genotypesame phenotype but different genotype
Seed shape and seed colorp
Round and wrinkled; yellow and green
RRYY x rryy
G t RY RY dGametes: RY;RY and ry;ry
F1: RrYy self pollinate
RrYy x RrYyRrYy x RrYy
Gametes (dependent assortment): RY;ry
and RY; ryy
F2?
Are they transmitted as package?
Will Y d R d d t t th ?
DihybridDihybrid crosscross
Will Y and R; and r and y stay together?
DihybridDihybrid crosscross
Probabilityobab y
The number of ways an event can occur
total number of possible outcomesp
ranges from 0 to 1
MendelianMendelian inheritance reflectsinheritance reflects
rules of probabilityrules of probabilityrules of probabilityrules of probability
Product Ruleodu u
◦ For independent events
Sum Rule
◦ Events that can occur in two or more different
ways
What is the probability of getting a heterozygoteWhat is the probability of getting a heterozygote
(Pp) in this cross?
A B
Pp x Pp
1. P= ½ ; p= ½ ¼
2 p= ½; P = ½ ¼2. p= ½; P = ½ ¼
1. Incomplete dominance
NonNon mendelianmendelian inheritanceinheritanceNonNon mendelianmendelian inheritanceinheritance
2. Multiple alleles- more than 2 allelesu p a s o a a s
3. Polygenic inheritance
4. pleiotropy- single gene controls morep o opy s g g o o s o
than one character
eg. one gene affects corolla, anther, calyx,
leaf and capsule of tobacco
5. Linked genes- genes are on the same5 d g s g s a o sa
chromosome
6. Cytoplasmic inheritance6 Cy op as a
- Maternal inheritance
- Extra nuclear genesg
7. Epistasis – Cucurbita pepop s as s Cu u b a p po

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Plant genetics

  • 1. Plant GeneticsPlant Genetics JJA Obico, Department of BiologyJJA Obico, Department of Biology University of the Philippines Manila
  • 2. Study of heredity and hereditary variationS udy o d y a d d a y a a o Genes - hereditary units - Segments of DNA Chromosomes - DNA + proteins GeneticsGeneticsGeneticsGenetics
  • 3.
  • 4. A. Interphasep as 1. Gap 1 or G1 phase 2. Synthesis phase or S phase 3. G2 phase B. M-phase (Mitosis or Meiosis) Cell CycleCell CycleCell CycleCell Cycle
  • 5. 1. G1 h th ll d◦ where the cell spends most of its functional life ◦ Performs metabolic acitivities 2. S-phase ◦ DNA molecules are copied or replicatedcopied or replicated ◦ from single stranded DNA in G1 phase to double stranded DNAstranded DNA 3. G2 ◦ preparing for the actual division events InterphaseInterphase (much protein synthesis- tubulin) InterphaseInterphase
  • 6.
  • 7.
  • 9. Asexual or Sexual Reproduction?p 1. Crossing over 2. Independent assortment of chromosomes 3. Random fertilization SOURCES OF VARIATIONSOURCES OF VARIATIONSOURCES OF VARIATIONSOURCES OF VARIATION
  • 11. Independent assortment of chromosomesIndependent assortment of chromosomesIndependent assortment of chromosomesIndependent assortment of chromosomes
  • 12. 2n 223 The no. of possible combination of maternal and paternal chromosomes in the gametes Random fertilization of an ovumRandom fertilization of an ovum by a spermby a spermby a spermby a sperm
  • 13.
  • 14.
  • 15.
  • 16. True-breeding plantsu b d g p a s Two varities ◦ Purple flower ◦ White flower Hybridization M h b idMonohybrid cross
  • 17.
  • 18. Whatever the factor that caused thea a o a aus d purple color was more powerful than the factor for white color Mendel said that the yellow factor "d i t d" th f t"dominated" the green factor.
  • 19. 1. Alternative version of genes account fora s o o g s a ou o variations in inherited character Mendel’s findingsMendel’s findingsMendel s findingsMendel s findings
  • 20. 2. For each character, an organism inheritso a a a , a o ga s s 2 alleles: one from each parent Homozygous– identical alleles Heterozygous– different alleles
  • 21. 3. Dominant allele – fully expressed3 o a a u y p ss d Recessive allele – masked Dominant- uppercase Recessive – lowercase P- purple; p- white
  • 22. 4. Two alleles for each character segregateo a s o a a a s g ga during gamete production – LAW of SEGREGATION AN ovum or sperm get only ONE of alleles f ll thof all the genes
  • 23.
  • 24. PUNNETT squareU squa - A tool for predicting the results of a genetic cross P P p Pp Pp p Pp Pp What will be the physicalWhat will be the physical appearance?appearance?appearance?appearance?
  • 25. Self pollinate Pp individualsS po a p d dua s P p P PP Pp P Pp pp
  • 26. Pp, PPPp, PP-- same phenotype but different genotypesame phenotype but different genotype
  • 27.
  • 28. Seed shape and seed colorp Round and wrinkled; yellow and green RRYY x rryy G t RY RY dGametes: RY;RY and ry;ry F1: RrYy self pollinate RrYy x RrYyRrYy x RrYy Gametes (dependent assortment): RY;ry and RY; ryy F2? Are they transmitted as package? Will Y d R d d t t th ? DihybridDihybrid crosscross Will Y and R; and r and y stay together? DihybridDihybrid crosscross
  • 29.
  • 30.
  • 31. Probabilityobab y The number of ways an event can occur total number of possible outcomesp ranges from 0 to 1 MendelianMendelian inheritance reflectsinheritance reflects rules of probabilityrules of probabilityrules of probabilityrules of probability
  • 32. Product Ruleodu u ◦ For independent events Sum Rule ◦ Events that can occur in two or more different ways What is the probability of getting a heterozygoteWhat is the probability of getting a heterozygote (Pp) in this cross? A B Pp x Pp 1. P= ½ ; p= ½ ¼ 2 p= ½; P = ½ ¼2. p= ½; P = ½ ¼
  • 33. 1. Incomplete dominance NonNon mendelianmendelian inheritanceinheritanceNonNon mendelianmendelian inheritanceinheritance
  • 34. 2. Multiple alleles- more than 2 allelesu p a s o a a s
  • 36.
  • 37. 4. pleiotropy- single gene controls morep o opy s g g o o s o than one character eg. one gene affects corolla, anther, calyx, leaf and capsule of tobacco
  • 38. 5. Linked genes- genes are on the same5 d g s g s a o sa chromosome
  • 39. 6. Cytoplasmic inheritance6 Cy op as a - Maternal inheritance - Extra nuclear genesg
  • 40. 7. Epistasis – Cucurbita pepop s as s Cu u b a p po