molecular tax ppt

1. Applications of Genetics to Conservation Biology -Molecular Taxonomy -Population Genetics and Gene Flow -Relatedness (Kinship, Paternity, Individual ID)

4. Molecular Taxonomy -Using molecules (ie. DNA based techniques such as mitochondrial DNA or nuclear DNA) to define taxonomic units (species, subspecies, ESUs and MUs)

7. Population Genetics and Gene Flow -Compare genetic traits among populations -Resolve substructure among populations -Infer movement patterns among individuals -Infer historical events for species

9. Relatedness (Kinship, Paternity and Individual ID) -Application of molecular genetic techniques (using hypervariable, repetitive DNA such as DNA fingerprinting and microsatellites) to questions of kinship, paternity, or individual ID

13. Subspecies Taxonomy and Gene Flow: Puma (cougar, mountain lion)

14. 32 Puma subspecies, as of the early 1900s

17. Mitochondrial DNA Haplotypes (in a geographical cline)

18. Microsatellite Alleles at FCA008

19. -Geographic clustering of individuals ~Six groups identified 2 distance methods agree

20. Major restrictions to gene flow: -Amazon River -Rio Parana -Rio Negro -Andes?

23. -Ancestor to puma crosses land-bridge ~2-3 Mya -Puma origin in Brazillian Highlands ~300,000 ya

24. 2 Major historical radiations -One locally distributed -One broad ranging

28. Paternity Application: Madagascar Fish-Eagle Ruth Tingay, PhD candidate U of Nottingham,

29. Antsalova wetland region of western Madagascar

31. DNA Fingerprinting Alleles at 4 nests (6 representative bands out of 34)