Question 3 A region of the cystic fibrosis gene from a person with c.pdf

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A region of the cystic fibrosis gene was sequenced from someone with and without the disease. The gel pattern shows an extra band in the sequence from the person with cystic fibrosis. This indicates a deletion mutation that causes a frameshift, changing the amino acid sequence encoded by this region of the gene.

Question 3: A region of the cystic fibrosis gene from a person with cystic fibrosis (CF) and the
same region from someone without CF are sequenced by the dideoxy sequencing method. Based
on the resulting gel pattern shown below, what is the nature of the mutation that is present in the
DNA of the person with CF? Does this affect the amino acid sequence encoded by this region of
the gene, and if so, how? You may assume that you have sequenced the coding strand. The frame
is indicated to you on the side of the gel.

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