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BACTERIAL GENOME ANNOTATION
            SERVICE
www.era7bioinformatics.com
Our service highlights

   Specifically designed for         data

   Based on

                           So it’s       and

   It’s                  according to our customer needs




www.era7bioinformatics.com
specifically designed for    data




www.era7bioinformatics.com
specifically designed for    data




www.era7bioinformatics.com
specifically designed for    data




www.era7bioinformatics.com
specifically designed for    data




www.era7bioinformatics.com
specifically designed for                data

                 Error-tolerant system
                 It can handle sequencing errors that may cause:

                          Indels, Frameshifts, Base substitution




www.era7bioinformatics.com
specifically designed for                        data

                       Error-tolerant system
                       It can handle sequencing errors that may cause:

                                 Indels, Frameshifts, Base substitution

Works with
Large number of contigs: There’s no need to have the genome closed

Large Bacterial Genomes: Up to 7Mb genomes annotated successfully


www.era7bioinformatics.com
We use


So our service is
In 24 hours you can have your bacterial genome analyzed




www.era7bioinformatics.com
We use


So our service is
In 24 hours you can have your bacterial genome analyzed



 and
 whether you need to annotate 1 genome or 100 we can handle it



www.era7bioinformatics.com
It’s


The annotation can be focused on particular aspects you
  may be interested in:

                     The presence of a particular enzime activity
                                       Resistance to antibiotics
                                              Mobile elements…




www.era7bioinformatics.com
annotation


The annotation system is based on the latest releases of

                              Uniprot and GenBank databases

                          So you’ll get an up to date annotation




www.era7bioinformatics.com
www.era7bioinformatics.com
Sequencing data




www.era7bioinformatics.com
Sequencing data




 Info about your
project, your needs


www.era7bioinformatics.com
Sequencing data




 Info about your      Reference Proteins
project, your needs       and RNAs


www.era7bioinformatics.com
Manual selection by
   an expert in bacterial
        genomics            Sequencing data




 Info about your            Reference Proteins
project, your needs             and RNAs


www.era7bioinformatics.com
Manual selection by
   an expert in bacterial
        genomics            Sequencing data

                                                 BG7




 Info about your            Reference Proteins
project, your needs             and RNAs


www.era7bioinformatics.com
Preliminary
                                                         results




    Manual selection by
   an expert in bacterial
        genomics            Sequencing data

                                                 BG7




 Info about your            Reference Proteins
project, your needs             and RNAs


www.era7bioinformatics.com
Preliminary
                                                         results



                                                               Automated
                                                                QC Tests
    Manual selection by
   an expert in bacterial
        genomics            Sequencing data
                                                         Results
                                                 BG7   Passing QC
                                                          tests




 Info about your            Reference Proteins
project, your needs             and RNAs


www.era7bioinformatics.com
Preliminary
                                                         results



                                                               Automated
                                                                QC Tests
    Manual selection by
   an expert in bacterial
        genomics            Sequencing data
                                                         Results
                                                 BG7   Passing QC
                                                          tests




                                                        Definitive
                                                         results
 Info about your            Reference Proteins
project, your needs             and RNAs


www.era7bioinformatics.com
Preliminary
                                                         results



                                                               Automated
                                                                QC Tests
    Manual selection by
   an expert in bacterial
        genomics            Sequencing data                                An expert in
                                                         Results             Bacterial
                                                 BG7   Passing QC            genomics
                                                          tests             checks that
                                                                           everything is
                                                                                OK




                                                        Definitive
                                                         results
 Info about your            Reference Proteins
project, your needs             and RNAs


www.era7bioinformatics.com
Preliminary
                                                           results



                                                                 Automated
                                                                  QC Tests
    Manual selection by
   an expert in bacterial
        genomics            Sequencing data                                  An expert in
                                                           Results             Bacterial
                                                 BG7     Passing QC            genomics
                                                            tests             checks that
                                                                             everything is
                                                                                  OK




                                                          Definitive
                                                           results
 Info about your
project, your needs
                            Reference Proteins
                                and RNAs
                                                 24 hr
www.era7bioinformatics.com
Preliminary
                                                           results



                                                                 Automated
                                                                  QC Tests
    Manual selection by
   an expert in bacterial
        genomics            Sequencing data                                  An expert in
                                                           Results             Bacterial
                                                 BG7     Passing QC            genomics
                                                            tests             checks that
                                                                             everything is
                                                                                  OK




                                                          Definitive
                                                           results
 Info about your
project, your needs
                            Reference Proteins
                                and RNAs
                                                 24 hr
www.era7bioinformatics.com
A
                                         Input contigs (nt)
                                 B
                                     C
  BG7 System




www.era7bioinformatics.com
A
                                                                        Input contigs (nt)
                                                               B
                                                                   C
  BG7 System

                              A                            B                   C




                             Input contigs translated into the 6 reading frames (aa)

www.era7bioinformatics.com
A
                                                                        Input contigs (nt)
                                                               B
                                                                   C
  BG7 System

                              A                            B                   C




  Reference Proteins (aa)




                             Input contigs translated into the 6 reading frames (aa)

www.era7bioinformatics.com
A
                                                                                      Input contigs (nt)
                                                                             B
                                                                                 C
  BG7 System

                                            A                            B                   C




  Reference Proteins (aa)
  are searched in the translated contigs




                                           Input contigs translated into the 6 reading frames (aa)

www.era7bioinformatics.com
Genes are defined based on sequence
                             similarity to proteins

  BG7 System




   A




                        B



           C




www.era7bioinformatics.com
Genes are defined based on sequence
                             similarity to proteins

  BG7 System




   A




                        B



           C




www.era7bioinformatics.com
Genes are defined based on sequence
                             similarity to proteins

  BG7 System
                             RNAs and then searched for in the contigs
                             and annotated




   A




                        B



           C




www.era7bioinformatics.com
Genes are defined based on sequence
                             similarity to proteins

  BG7 System
                             RNAs and then searched for in the contigs
                             and annotated




   A




                        B



           C




www.era7bioinformatics.com
What would you get at the end?


     A Fast and Accurate genome annotation rich in functional data:
       GO, Interpro…

     Deliverables in several formats:
       XML, GenBank, GFF, Fasta, .xls (Excel), .doc (Word), .txt

     An user friendly annotation viewer




www.era7bioinformatics.com
And some important things to know:

    Working with us means
     No IP over the results, they’re all yours
     CDA
     Bill by project



    Of course, there are other ways…
       research collaboration



www.era7bioinformatics.com
Follow us on:
         eduardopareja marina_manrique rtobes

         Eduardo Pareja Marina Manrique Raquel Tobes

          Marina Manrique Raquel Tobes


Join our NGS group at LinkedIn

                                           epareja@era7.com
                                            rtobes@era7.com
                                         mmanrique@era7.com
www.era7bioinformatics.com

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Era7 Bacterial Genome Annotation

  • 3. Our service highlights Specifically designed for data Based on So it’s and It’s according to our customer needs www.era7bioinformatics.com
  • 4. specifically designed for data www.era7bioinformatics.com
  • 5. specifically designed for data www.era7bioinformatics.com
  • 6. specifically designed for data www.era7bioinformatics.com
  • 7. specifically designed for data www.era7bioinformatics.com
  • 8. specifically designed for data Error-tolerant system It can handle sequencing errors that may cause: Indels, Frameshifts, Base substitution www.era7bioinformatics.com
  • 9. specifically designed for data Error-tolerant system It can handle sequencing errors that may cause: Indels, Frameshifts, Base substitution Works with Large number of contigs: There’s no need to have the genome closed Large Bacterial Genomes: Up to 7Mb genomes annotated successfully www.era7bioinformatics.com
  • 10. We use So our service is In 24 hours you can have your bacterial genome analyzed www.era7bioinformatics.com
  • 11. We use So our service is In 24 hours you can have your bacterial genome analyzed and whether you need to annotate 1 genome or 100 we can handle it www.era7bioinformatics.com
  • 12. It’s The annotation can be focused on particular aspects you may be interested in: The presence of a particular enzime activity Resistance to antibiotics Mobile elements… www.era7bioinformatics.com
  • 13. annotation The annotation system is based on the latest releases of Uniprot and GenBank databases So you’ll get an up to date annotation www.era7bioinformatics.com
  • 16. Sequencing data Info about your project, your needs www.era7bioinformatics.com
  • 17. Sequencing data Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 18. Manual selection by an expert in bacterial genomics Sequencing data Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 19. Manual selection by an expert in bacterial genomics Sequencing data BG7 Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 20. Preliminary results Manual selection by an expert in bacterial genomics Sequencing data BG7 Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 21. Preliminary results Automated QC Tests Manual selection by an expert in bacterial genomics Sequencing data Results BG7 Passing QC tests Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 22. Preliminary results Automated QC Tests Manual selection by an expert in bacterial genomics Sequencing data Results BG7 Passing QC tests Definitive results Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 23. Preliminary results Automated QC Tests Manual selection by an expert in bacterial genomics Sequencing data An expert in Results Bacterial BG7 Passing QC genomics tests checks that everything is OK Definitive results Info about your Reference Proteins project, your needs and RNAs www.era7bioinformatics.com
  • 24. Preliminary results Automated QC Tests Manual selection by an expert in bacterial genomics Sequencing data An expert in Results Bacterial BG7 Passing QC genomics tests checks that everything is OK Definitive results Info about your project, your needs Reference Proteins and RNAs 24 hr www.era7bioinformatics.com
  • 25. Preliminary results Automated QC Tests Manual selection by an expert in bacterial genomics Sequencing data An expert in Results Bacterial BG7 Passing QC genomics tests checks that everything is OK Definitive results Info about your project, your needs Reference Proteins and RNAs 24 hr www.era7bioinformatics.com
  • 26. A Input contigs (nt) B C BG7 System www.era7bioinformatics.com
  • 27. A Input contigs (nt) B C BG7 System A B C Input contigs translated into the 6 reading frames (aa) www.era7bioinformatics.com
  • 28. A Input contigs (nt) B C BG7 System A B C Reference Proteins (aa) Input contigs translated into the 6 reading frames (aa) www.era7bioinformatics.com
  • 29. A Input contigs (nt) B C BG7 System A B C Reference Proteins (aa) are searched in the translated contigs Input contigs translated into the 6 reading frames (aa) www.era7bioinformatics.com
  • 30. Genes are defined based on sequence similarity to proteins BG7 System A B C www.era7bioinformatics.com
  • 31. Genes are defined based on sequence similarity to proteins BG7 System A B C www.era7bioinformatics.com
  • 32. Genes are defined based on sequence similarity to proteins BG7 System RNAs and then searched for in the contigs and annotated A B C www.era7bioinformatics.com
  • 33. Genes are defined based on sequence similarity to proteins BG7 System RNAs and then searched for in the contigs and annotated A B C www.era7bioinformatics.com
  • 34. What would you get at the end? A Fast and Accurate genome annotation rich in functional data: GO, Interpro… Deliverables in several formats: XML, GenBank, GFF, Fasta, .xls (Excel), .doc (Word), .txt An user friendly annotation viewer www.era7bioinformatics.com
  • 35. And some important things to know: Working with us means No IP over the results, they’re all yours CDA Bill by project Of course, there are other ways… research collaboration www.era7bioinformatics.com
  • 36. Follow us on: eduardopareja marina_manrique rtobes Eduardo Pareja Marina Manrique Raquel Tobes Marina Manrique Raquel Tobes Join our NGS group at LinkedIn epareja@era7.com rtobes@era7.com mmanrique@era7.com www.era7bioinformatics.com