8. specifically designed for data
Error-tolerant system
It can handle sequencing errors that may cause:
Indels, Frameshifts, Base substitution
www.era7bioinformatics.com
9. specifically designed for data
Error-tolerant system
It can handle sequencing errors that may cause:
Indels, Frameshifts, Base substitution
Works with
Large number of contigs: There’s no need to have the genome closed
Large Bacterial Genomes: Up to 7Mb genomes annotated successfully
www.era7bioinformatics.com
10. We use
So our service is
In 24 hours you can have your bacterial genome analyzed
www.era7bioinformatics.com
11. We use
So our service is
In 24 hours you can have your bacterial genome analyzed
and
whether you need to annotate 1 genome or 100 we can handle it
www.era7bioinformatics.com
12. It’s
The annotation can be focused on particular aspects you
may be interested in:
The presence of a particular enzime activity
Resistance to antibiotics
Mobile elements…
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13. annotation
The annotation system is based on the latest releases of
Uniprot and GenBank databases
So you’ll get an up to date annotation
www.era7bioinformatics.com
17. Sequencing data
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
18. Manual selection by
an expert in bacterial
genomics Sequencing data
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
19. Manual selection by
an expert in bacterial
genomics Sequencing data
BG7
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
20. Preliminary
results
Manual selection by
an expert in bacterial
genomics Sequencing data
BG7
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
21. Preliminary
results
Automated
QC Tests
Manual selection by
an expert in bacterial
genomics Sequencing data
Results
BG7 Passing QC
tests
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
22. Preliminary
results
Automated
QC Tests
Manual selection by
an expert in bacterial
genomics Sequencing data
Results
BG7 Passing QC
tests
Definitive
results
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
23. Preliminary
results
Automated
QC Tests
Manual selection by
an expert in bacterial
genomics Sequencing data An expert in
Results Bacterial
BG7 Passing QC genomics
tests checks that
everything is
OK
Definitive
results
Info about your Reference Proteins
project, your needs and RNAs
www.era7bioinformatics.com
24. Preliminary
results
Automated
QC Tests
Manual selection by
an expert in bacterial
genomics Sequencing data An expert in
Results Bacterial
BG7 Passing QC genomics
tests checks that
everything is
OK
Definitive
results
Info about your
project, your needs
Reference Proteins
and RNAs
24 hr
www.era7bioinformatics.com
25. Preliminary
results
Automated
QC Tests
Manual selection by
an expert in bacterial
genomics Sequencing data An expert in
Results Bacterial
BG7 Passing QC genomics
tests checks that
everything is
OK
Definitive
results
Info about your
project, your needs
Reference Proteins
and RNAs
24 hr
www.era7bioinformatics.com
26. A
Input contigs (nt)
B
C
BG7 System
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27. A
Input contigs (nt)
B
C
BG7 System
A B C
Input contigs translated into the 6 reading frames (aa)
www.era7bioinformatics.com
28. A
Input contigs (nt)
B
C
BG7 System
A B C
Reference Proteins (aa)
Input contigs translated into the 6 reading frames (aa)
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29. A
Input contigs (nt)
B
C
BG7 System
A B C
Reference Proteins (aa)
are searched in the translated contigs
Input contigs translated into the 6 reading frames (aa)
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30. Genes are defined based on sequence
similarity to proteins
BG7 System
A
B
C
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31. Genes are defined based on sequence
similarity to proteins
BG7 System
A
B
C
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32. Genes are defined based on sequence
similarity to proteins
BG7 System
RNAs and then searched for in the contigs
and annotated
A
B
C
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33. Genes are defined based on sequence
similarity to proteins
BG7 System
RNAs and then searched for in the contigs
and annotated
A
B
C
www.era7bioinformatics.com
34. What would you get at the end?
A Fast and Accurate genome annotation rich in functional data:
GO, Interpro…
Deliverables in several formats:
XML, GenBank, GFF, Fasta, .xls (Excel), .doc (Word), .txt
An user friendly annotation viewer
www.era7bioinformatics.com
35. And some important things to know:
Working with us means
No IP over the results, they’re all yours
CDA
Bill by project
Of course, there are other ways…
research collaboration
www.era7bioinformatics.com
36. Follow us on:
eduardopareja marina_manrique rtobes
Eduardo Pareja Marina Manrique Raquel Tobes
Marina Manrique Raquel Tobes
Join our NGS group at LinkedIn
epareja@era7.com
rtobes@era7.com
mmanrique@era7.com
www.era7bioinformatics.com