SlideShare una empresa de Scribd logo
1 de 24
Singapore grouper iridovirus (SGIV) induced
parapotosis-like cell death in host cells via the
activation of MAPK signalings
Qiwei Qin
South China Sea Institute of Oceanology
Chinese Academy of Sciences
2013.7.27
programmed cell death , PCD
PCD is natural and genetically regulated process of removing unneeded
or damaged cells, and it plays critical roles in the balance between host
defense and the invading pathogen during pathogen-host co-evolution.
Research background
(Dale E. Bredesen, 2007)
Morphological and biochemical characteristics of apoptosis and
parapoptosis (Sperandio et al., 2000)
Virus infection induced PCD
Virus infection induced apoptosis. Virus infection induced autophagic PCD
So far, no report on virus infection induced parapoptosis !
MAPK signaling pathways and PCD
MAPKs have been identified: extracellular signal-
regulated kinase (ERK), c-Jun N-terminal kinases
(JNK) and p38 MAPK ( Wada et al, 2004 )。
Mitogen-activated protein
kinase (MAPK) cascades
are evolutionary
conserved intracellular
signaling pathways that
regulate gene expression,
mitosis, proliferation,
metabolism and
programmed cell death.
MAPKs and virus infection
MAPK
(Han, Nat Immunol, 2006)
TLR could recognize the PAMPs of pathogens (virus, bacterial) and
trigger the host immune responses, including the activation of MAPK signaling pathways.
Virus infection could activate MAPK
pathways in mammalian and humans.
Aquatic animal
viruses activate
MAPK signaling
pathways ??
Iridovirus is one of the most important infectious
pathgens for maricultured grouper, Epinephelus spp !
Singapore grouper iridovirus (SGIV) isolated from diseased grouper is a
novel species of ranavirus, family iridovidae ( Qin et al., 2003).
1. How the SGIV interact with grouper host cells causing
cell death ?
2 、 What kind of host cell signaling pathways involve in
the virus infection ?
Huang et al, Aquaculture, 2009
SGIV infected grouper cells
Lai et al, J Fish Dis. 2008
GIV infected barramundi cells
Apoptosis Non-apoptosis
Whether SGIV infection induced PCD is cell type
dependent ?
Research results
1.SGIV infection induced different type of cell death and nucleus
morphology in host and non-host two fish cells
Cell types Characteristics
SGIV-EAGS rounded, congregated, remain attach, condensed
nuclei, non-apoptotic bodies
SGIV-FHM rounded, detached, apoptotic bodies observed
Cells Characteristics
SGIV-FHM DNA fragementation, DNA ladder, TUNEL positive
SGIV-EAGS no fragementation,no DNA ladder, TUNEL negetive
DNA fragmentation and DAN content analysis of
SGIV-infected two fish cells
SGIV-infectedEAGS:
appearance of cytoplamic
vaculoes, distended endoplasmic
reticulum (ER), large cluster of
swollen mitochondria.
SGIV-FHM:
ER swelling
Examination of PS externalization and caspae activities
The exposure of PS on cell surface is a general marker of apoptotic cells. The exposure of PS
only occurred on the FHM cell surface during SGIV infection.
Caspase-3 and -9 were activated in SGIV-infected FHM cells, but not in infected EAGS cells.
UV-inactivated SGIV had not effect on EAGS cells, but still evoked obviously
apoptosis in FHM cells.
Virus replication was essential for SGIV induced nonapoptotic cell
death, but not for apoptosis.
SGIV infection activated MAPK signaling
molecules in grouper cells
ERK, p38 MAPK and JNK molecules were phosphorylated at the different
stages during SGIV infection in host EAGS cells.
Inhibition of ERK and JNK activities could delay the CPE and reduce the virus production.
MAPK signaling pathways were involved in SGIV
replication in grouper host cells
Inhibition of JNK pathway could inhibit SGIV gene transcription and
protein synthesis. No obvious changes for inhibition of p38 MAPK.
JNK but not p38 MAPK is important for SGIV
transcription and protein synthesis
SGIV infection evoked increased the expression of IL-8, IRF-1 and
TNFa, inhibition of JNK resulted in the reduction of 3 immune
genes, inhibition of p38 MAPK only reduced the expression of
Fish immune genes were regulated by MAPKs
IL-8 IRF-1 TNF-a
Conclusions
SGIV infection induced nonapoptotic cell death-parapotosis
in grouper host cells.
MAPK signaling pathways involved in the SGIV
infection and replication in host cells.
ERK 、 JNK signals are essential for virus replication
and virus induced parapotosis
SGIV-activated MAPK pathways could modulate the expression of
immune genes and inflammatory cytokines
Related publications:
1. Huang X., Huang Y., Ouyang Z., Cai J., Qin Q. 2011. J Gen Virol.,
Jun; 92:1292-301.
2. Huang X., Huang Y., Ouyang Z., Xu L, Yan Y, Cui H, Han X, Qin Q.
2011. Apoptosis, Aug;16(8):831-45.
23
Acknowledges: National Basical Research
Program(973 program); NSFC; CAS
Thank you very muchThank you very much
for your attentionfor your attention !!

Más contenido relacionado

La actualidad más candente

Corona virus ppt by Manosh
Corona virus ppt by ManoshCorona virus ppt by Manosh
Corona virus ppt by Manoshremysuresh
 
short note on Covid-19
short note on Covid-19short note on Covid-19
short note on Covid-19Tamanna Syeda
 
Developing Ultra-Sensitive PCR Protocols for HIV Vaccine Research
Developing Ultra-Sensitive PCR Protocols for HIV Vaccine ResearchDeveloping Ultra-Sensitive PCR Protocols for HIV Vaccine Research
Developing Ultra-Sensitive PCR Protocols for HIV Vaccine ResearchKate Barlow
 
Presentation on covide19 and used drug machanism
Presentation on covide19 and used  drug machanismPresentation on covide19 and used  drug machanism
Presentation on covide19 and used drug machanismGauriShankar38
 
Lidiya Angelova-Duleva PhD thesis
Lidiya Angelova-Duleva PhD thesisLidiya Angelova-Duleva PhD thesis
Lidiya Angelova-Duleva PhD thesisguest962e0a
 
Genotype and phenotype of covid 19
Genotype and phenotype of covid 19Genotype and phenotype of covid 19
Genotype and phenotype of covid 19Bharati Somannavar
 
Transctiptomic Analysis- Samisti12.ppt
Transctiptomic Analysis- Samisti12.pptTransctiptomic Analysis- Samisti12.ppt
Transctiptomic Analysis- Samisti12.pptFarshad Hamid
 
7. molecular genetics
7. molecular genetics7. molecular genetics
7. molecular geneticsmartalpz
 
Sars co v-2-lc-introductory
Sars co v-2-lc-introductorySars co v-2-lc-introductory
Sars co v-2-lc-introductoryBindhuBrindha
 
113 phillip d. zamore - 7691995 - in vivo production of small interfering r...
113   phillip d. zamore - 7691995 - in vivo production of small interfering r...113   phillip d. zamore - 7691995 - in vivo production of small interfering r...
113 phillip d. zamore - 7691995 - in vivo production of small interfering r...Mello_Patent_Registry
 
128 phillip d. zamore - 8232260 - in vivo production of small interfering r...
128   phillip d. zamore - 8232260 - in vivo production of small interfering r...128   phillip d. zamore - 8232260 - in vivo production of small interfering r...
128 phillip d. zamore - 8232260 - in vivo production of small interfering r...Mello_Patent_Registry
 
Structure and replication .
Structure and replication . Structure and replication .
Structure and replication . shrutiPATIL130
 
Biological properties of African swine fever virus Odintsovo 02/14 isolate an...
Biological properties of African swine fever virus Odintsovo 02/14 isolate an...Biological properties of African swine fever virus Odintsovo 02/14 isolate an...
Biological properties of African swine fever virus Odintsovo 02/14 isolate an...Agriculture Journal IJOEAR
 
CORONAVIRUS AKA SARS PATENT
CORONAVIRUS AKA SARS PATENT CORONAVIRUS AKA SARS PATENT
CORONAVIRUS AKA SARS PATENT ICJ-ICC
 
Next Generation Sequencing- NGS for COVID19 PPT
Next Generation Sequencing- NGS for COVID19 PPTNext Generation Sequencing- NGS for COVID19 PPT
Next Generation Sequencing- NGS for COVID19 PPTMesele Tilahun
 
SARS-COV-2 detection by RNAscope technology
SARS-COV-2 detection by RNAscope technologySARS-COV-2 detection by RNAscope technology
SARS-COV-2 detection by RNAscope technologyCOSMO BIO
 
an mrna vaccine aganist sars cov 2
an mrna vaccine aganist sars cov 2 an mrna vaccine aganist sars cov 2
an mrna vaccine aganist sars cov 2 antomathew8
 
Us7279327 Methods for producing recombinant coronavirus
Us7279327 Methods for producing recombinant coronavirusUs7279327 Methods for producing recombinant coronavirus
Us7279327 Methods for producing recombinant coronavirusCultura Arte Sociedad (Cuarso)
 

La actualidad más candente (20)

Corona virus ppt by Manosh
Corona virus ppt by ManoshCorona virus ppt by Manosh
Corona virus ppt by Manosh
 
short note on Covid-19
short note on Covid-19short note on Covid-19
short note on Covid-19
 
Developing Ultra-Sensitive PCR Protocols for HIV Vaccine Research
Developing Ultra-Sensitive PCR Protocols for HIV Vaccine ResearchDeveloping Ultra-Sensitive PCR Protocols for HIV Vaccine Research
Developing Ultra-Sensitive PCR Protocols for HIV Vaccine Research
 
Presentation on covide19 and used drug machanism
Presentation on covide19 and used  drug machanismPresentation on covide19 and used  drug machanism
Presentation on covide19 and used drug machanism
 
Lidiya Angelova-Duleva PhD thesis
Lidiya Angelova-Duleva PhD thesisLidiya Angelova-Duleva PhD thesis
Lidiya Angelova-Duleva PhD thesis
 
Genotype and phenotype of covid 19
Genotype and phenotype of covid 19Genotype and phenotype of covid 19
Genotype and phenotype of covid 19
 
Transctiptomic Analysis- Samisti12.ppt
Transctiptomic Analysis- Samisti12.pptTransctiptomic Analysis- Samisti12.ppt
Transctiptomic Analysis- Samisti12.ppt
 
Raju Paper
Raju PaperRaju Paper
Raju Paper
 
7. molecular genetics
7. molecular genetics7. molecular genetics
7. molecular genetics
 
Sars co v-2-lc-introductory
Sars co v-2-lc-introductorySars co v-2-lc-introductory
Sars co v-2-lc-introductory
 
113 phillip d. zamore - 7691995 - in vivo production of small interfering r...
113   phillip d. zamore - 7691995 - in vivo production of small interfering r...113   phillip d. zamore - 7691995 - in vivo production of small interfering r...
113 phillip d. zamore - 7691995 - in vivo production of small interfering r...
 
PCR Congress Presentation 05 DEC 2017 CNK
PCR Congress Presentation 05 DEC 2017 CNKPCR Congress Presentation 05 DEC 2017 CNK
PCR Congress Presentation 05 DEC 2017 CNK
 
128 phillip d. zamore - 8232260 - in vivo production of small interfering r...
128   phillip d. zamore - 8232260 - in vivo production of small interfering r...128   phillip d. zamore - 8232260 - in vivo production of small interfering r...
128 phillip d. zamore - 8232260 - in vivo production of small interfering r...
 
Structure and replication .
Structure and replication . Structure and replication .
Structure and replication .
 
Biological properties of African swine fever virus Odintsovo 02/14 isolate an...
Biological properties of African swine fever virus Odintsovo 02/14 isolate an...Biological properties of African swine fever virus Odintsovo 02/14 isolate an...
Biological properties of African swine fever virus Odintsovo 02/14 isolate an...
 
CORONAVIRUS AKA SARS PATENT
CORONAVIRUS AKA SARS PATENT CORONAVIRUS AKA SARS PATENT
CORONAVIRUS AKA SARS PATENT
 
Next Generation Sequencing- NGS for COVID19 PPT
Next Generation Sequencing- NGS for COVID19 PPTNext Generation Sequencing- NGS for COVID19 PPT
Next Generation Sequencing- NGS for COVID19 PPT
 
SARS-COV-2 detection by RNAscope technology
SARS-COV-2 detection by RNAscope technologySARS-COV-2 detection by RNAscope technology
SARS-COV-2 detection by RNAscope technology
 
an mrna vaccine aganist sars cov 2
an mrna vaccine aganist sars cov 2 an mrna vaccine aganist sars cov 2
an mrna vaccine aganist sars cov 2
 
Us7279327 Methods for producing recombinant coronavirus
Us7279327 Methods for producing recombinant coronavirusUs7279327 Methods for producing recombinant coronavirus
Us7279327 Methods for producing recombinant coronavirus
 

Similar a Singapore grouper iridovirus induced parapoptosis-like death in host cells via the activation of MAPK signaling

H. Zheng - Innate immune evasion by foot-and-mouth disease virus
H. Zheng - Innate immune evasion by foot-and-mouth disease virus H. Zheng - Innate immune evasion by foot-and-mouth disease virus
H. Zheng - Innate immune evasion by foot-and-mouth disease virus EuFMD
 
Sigma xi presentation final1
Sigma xi presentation   final1Sigma xi presentation   final1
Sigma xi presentation final1mukta936
 
Adenosine deaminase (ADA) immunodeficiency
Adenosine deaminase (ADA) immunodeficiencyAdenosine deaminase (ADA) immunodeficiency
Adenosine deaminase (ADA) immunodeficiencyAref Farokhi Fard
 
Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...
Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...
Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...Mr. Suresh R. Jambagi
 
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...Allyson Luo
 
Modulation of pluripotency in the porcine embryo and i ps cells (Dec.27,2012)
Modulation of pluripotency in the  porcine embryo and i ps cells (Dec.27,2012) Modulation of pluripotency in the  porcine embryo and i ps cells (Dec.27,2012)
Modulation of pluripotency in the porcine embryo and i ps cells (Dec.27,2012) Ahmad Usama
 
gene silencing-for disease managment
gene silencing-for disease managmentgene silencing-for disease managment
gene silencing-for disease managmentBalaji Rathod
 
Cancer Signal Transduction
Cancer Signal TransductionCancer Signal Transduction
Cancer Signal TransductionDeepika Tripathi
 
Cancer signal-transduction
Cancer signal-transductionCancer signal-transduction
Cancer signal-transductionDeepika Tripathi
 
Preprint book rbd targeted covid vaccine and full length spike protein vacc...
Preprint  book  rbd targeted covid vaccine and full length spike protein vacc...Preprint  book  rbd targeted covid vaccine and full length spike protein vacc...
Preprint book rbd targeted covid vaccine and full length spike protein vacc...M. Luisetto Pharm.D.Spec. Pharmacology
 
In vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic repliconIn vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic repliconBinodGupta27
 
In vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic repliconIn vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic repliconBinodGupta27
 
Role of Autophagy in Plant-Pathogen interaction.pptx
Role of Autophagy in Plant-Pathogen interaction.pptxRole of Autophagy in Plant-Pathogen interaction.pptx
Role of Autophagy in Plant-Pathogen interaction.pptxVajrammaBoggala
 

Similar a Singapore grouper iridovirus induced parapoptosis-like death in host cells via the activation of MAPK signaling (20)

H. Zheng - Innate immune evasion by foot-and-mouth disease virus
H. Zheng - Innate immune evasion by foot-and-mouth disease virus H. Zheng - Innate immune evasion by foot-and-mouth disease virus
H. Zheng - Innate immune evasion by foot-and-mouth disease virus
 
Zakir hcv
Zakir hcvZakir hcv
Zakir hcv
 
Sigma xi presentation final1
Sigma xi presentation   final1Sigma xi presentation   final1
Sigma xi presentation final1
 
Adenosine deaminase (ADA) immunodeficiency
Adenosine deaminase (ADA) immunodeficiencyAdenosine deaminase (ADA) immunodeficiency
Adenosine deaminase (ADA) immunodeficiency
 
GKA deel 2 college 3
GKA deel 2 college 3GKA deel 2 college 3
GKA deel 2 college 3
 
publication 3
publication 3publication 3
publication 3
 
Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...
Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...
Pathogen-Driven Proteomic Changes in Haemolymph of NPV-Infected Silkworm Bomb...
 
Xie X et al 2014
Xie X et al 2014Xie X et al 2014
Xie X et al 2014
 
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...
Enhancing Solubilization of Hydrophobic ORF3 Product of HEV in Bacterial Expr...
 
1584-09
1584-091584-09
1584-09
 
sag1
sag1sag1
sag1
 
Modulation of pluripotency in the porcine embryo and i ps cells (Dec.27,2012)
Modulation of pluripotency in the  porcine embryo and i ps cells (Dec.27,2012) Modulation of pluripotency in the  porcine embryo and i ps cells (Dec.27,2012)
Modulation of pluripotency in the porcine embryo and i ps cells (Dec.27,2012)
 
gene silencing-for disease managment
gene silencing-for disease managmentgene silencing-for disease managment
gene silencing-for disease managment
 
Cancer Signal Transduction
Cancer Signal TransductionCancer Signal Transduction
Cancer Signal Transduction
 
Cancer signal-transduction
Cancer signal-transductionCancer signal-transduction
Cancer signal-transduction
 
Preprint book rbd targeted covid vaccine and full length spike protein vacc...
Preprint  book  rbd targeted covid vaccine and full length spike protein vacc...Preprint  book  rbd targeted covid vaccine and full length spike protein vacc...
Preprint book rbd targeted covid vaccine and full length spike protein vacc...
 
In vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic repliconIn vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic replicon
 
In vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic repliconIn vitro transcription and transfection of HCV genomic replicon
In vitro transcription and transfection of HCV genomic replicon
 
Reverse genetics
Reverse geneticsReverse genetics
Reverse genetics
 
Role of Autophagy in Plant-Pathogen interaction.pptx
Role of Autophagy in Plant-Pathogen interaction.pptxRole of Autophagy in Plant-Pathogen interaction.pptx
Role of Autophagy in Plant-Pathogen interaction.pptx
 

Más de mgray11

Evaluating Disease and Immunocompetency in Threatened Hellbenders
Evaluating Disease and Immunocompetency in Threatened HellbendersEvaluating Disease and Immunocompetency in Threatened Hellbenders
Evaluating Disease and Immunocompetency in Threatened Hellbendersmgray11
 
EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...
EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...
EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...mgray11
 
Pathogens vs. Pesticides: The Threat to Eastern Hellbenders
Pathogens vs. Pesticides: The Threat to Eastern HellbendersPathogens vs. Pesticides: The Threat to Eastern Hellbenders
Pathogens vs. Pesticides: The Threat to Eastern Hellbendersmgray11
 
Batrachochytrium salamandrivorans: Determining the Risk to North America
Batrachochytrium salamandrivorans: Determining the Risk to North AmericaBatrachochytrium salamandrivorans: Determining the Risk to North America
Batrachochytrium salamandrivorans: Determining the Risk to North Americamgray11
 
Averting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threat
Averting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threatAverting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threat
Averting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threatmgray11
 
Sample collection and shipping: what's important and why
Sample collection and shipping: what's important and whySample collection and shipping: what's important and why
Sample collection and shipping: what's important and whymgray11
 
Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...
Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...
Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...mgray11
 
A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...
A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...
A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...mgray11
 
Batrachochytrium salamandrivorans: A review of policy responses in North America
Batrachochytrium salamandrivorans: A review of policy responses in North AmericaBatrachochytrium salamandrivorans: A review of policy responses in North America
Batrachochytrium salamandrivorans: A review of policy responses in North Americamgray11
 
First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...
First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...
First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...mgray11
 
Ranavirus Surveillance Methods
Ranavirus Surveillance MethodsRanavirus Surveillance Methods
Ranavirus Surveillance Methodsmgray11
 
Global Ranavirus Consortium
Global Ranavirus ConsortiumGlobal Ranavirus Consortium
Global Ranavirus Consortiummgray11
 
A pathogen lurking in the darkness
A pathogen lurking in the darknessA pathogen lurking in the darkness
A pathogen lurking in the darknessmgray11
 
Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...
Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...
Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...mgray11
 
Ecology of Ranaviruses: State of Understanding
Ecology of Ranaviruses: State of UnderstandingEcology of Ranaviruses: State of Understanding
Ecology of Ranaviruses: State of Understandingmgray11
 
Ranaviruses: Cry Wolf or Real Threat?
Ranaviruses: Cry Wolf or Real Threat?Ranaviruses: Cry Wolf or Real Threat?
Ranaviruses: Cry Wolf or Real Threat?mgray11
 
Ranavirus in Costa Rica
Ranavirus in Costa RicaRanavirus in Costa Rica
Ranavirus in Costa Ricamgray11
 

Más de mgray11 (20)

Evaluating Disease and Immunocompetency in Threatened Hellbenders
Evaluating Disease and Immunocompetency in Threatened HellbendersEvaluating Disease and Immunocompetency in Threatened Hellbenders
Evaluating Disease and Immunocompetency in Threatened Hellbenders
 
EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...
EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...
EASTERN HELLBENDER (CRYPTOBRANCHUS ALLEGANIENSIS) CURRENT AND FUTURE RESEARCH...
 
Pathogens vs. Pesticides: The Threat to Eastern Hellbenders
Pathogens vs. Pesticides: The Threat to Eastern HellbendersPathogens vs. Pesticides: The Threat to Eastern Hellbenders
Pathogens vs. Pesticides: The Threat to Eastern Hellbenders
 
Batrachochytrium salamandrivorans: Determining the Risk to North America
Batrachochytrium salamandrivorans: Determining the Risk to North AmericaBatrachochytrium salamandrivorans: Determining the Risk to North America
Batrachochytrium salamandrivorans: Determining the Risk to North America
 
Averting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threat
Averting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threatAverting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threat
Averting a biodiversity crisis: AmphibiaWeb addresses the new Bsal threat
 
Sample collection and shipping: what's important and why
Sample collection and shipping: what's important and whySample collection and shipping: what's important and why
Sample collection and shipping: what's important and why
 
Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...
Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...
Risk Assessment of Batrachochytrium salamandrivorans Introduction into the Un...
 
A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...
A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...
A brief introduction to Batrachochytrium salamandrivorans (Bsal) and the thre...
 
Batrachochytrium salamandrivorans: A review of policy responses in North America
Batrachochytrium salamandrivorans: A review of policy responses in North AmericaBatrachochytrium salamandrivorans: A review of policy responses in North America
Batrachochytrium salamandrivorans: A review of policy responses in North America
 
First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...
First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...
First Steps Toward a National Strategic Plan for Batrachochytrium salamandriv...
 
Ranavirus Surveillance Methods
Ranavirus Surveillance MethodsRanavirus Surveillance Methods
Ranavirus Surveillance Methods
 
Global Ranavirus Consortium
Global Ranavirus ConsortiumGlobal Ranavirus Consortium
Global Ranavirus Consortium
 
A pathogen lurking in the darkness
A pathogen lurking in the darknessA pathogen lurking in the darkness
A pathogen lurking in the darkness
 
Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...
Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...
Ranavirus: an emerging pathogen in amphibian, fish and reptile populations in...
 
Ecology of Ranaviruses: State of Understanding
Ecology of Ranaviruses: State of UnderstandingEcology of Ranaviruses: State of Understanding
Ecology of Ranaviruses: State of Understanding
 
Ranaviruses: Cry Wolf or Real Threat?
Ranaviruses: Cry Wolf or Real Threat?Ranaviruses: Cry Wolf or Real Threat?
Ranaviruses: Cry Wolf or Real Threat?
 
Spitzen
SpitzenSpitzen
Spitzen
 
Mazzoni
MazzoniMazzoni
Mazzoni
 
Ranavirus in Costa Rica
Ranavirus in Costa RicaRanavirus in Costa Rica
Ranavirus in Costa Rica
 
Brunner
BrunnerBrunner
Brunner
 

Último

Micro-Scholarship, What it is, How can it help me.pdf
Micro-Scholarship, What it is, How can it help me.pdfMicro-Scholarship, What it is, How can it help me.pdf
Micro-Scholarship, What it is, How can it help me.pdfPoh-Sun Goh
 
Single or Multiple melodic lines structure
Single or Multiple melodic lines structureSingle or Multiple melodic lines structure
Single or Multiple melodic lines structuredhanjurrannsibayan2
 
Fostering Friendships - Enhancing Social Bonds in the Classroom
Fostering Friendships - Enhancing Social Bonds  in the ClassroomFostering Friendships - Enhancing Social Bonds  in the Classroom
Fostering Friendships - Enhancing Social Bonds in the ClassroomPooky Knightsmith
 
Understanding Accommodations and Modifications
Understanding  Accommodations and ModificationsUnderstanding  Accommodations and Modifications
Understanding Accommodations and ModificationsMJDuyan
 
Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...
Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...
Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...ZurliaSoop
 
How to setup Pycharm environment for Odoo 17.pptx
How to setup Pycharm environment for Odoo 17.pptxHow to setup Pycharm environment for Odoo 17.pptx
How to setup Pycharm environment for Odoo 17.pptxCeline George
 
Plant propagation: Sexual and Asexual propapagation.pptx
Plant propagation: Sexual and Asexual propapagation.pptxPlant propagation: Sexual and Asexual propapagation.pptx
Plant propagation: Sexual and Asexual propapagation.pptxUmeshTimilsina1
 
SOC 101 Demonstration of Learning Presentation
SOC 101 Demonstration of Learning PresentationSOC 101 Demonstration of Learning Presentation
SOC 101 Demonstration of Learning Presentationcamerronhm
 
COMMUNICATING NEGATIVE NEWS - APPROACHES .pptx
COMMUNICATING NEGATIVE NEWS - APPROACHES .pptxCOMMUNICATING NEGATIVE NEWS - APPROACHES .pptx
COMMUNICATING NEGATIVE NEWS - APPROACHES .pptxannathomasp01
 
2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx
2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx
2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptxMaritesTamaniVerdade
 
Towards a code of practice for AI in AT.pptx
Towards a code of practice for AI in AT.pptxTowards a code of practice for AI in AT.pptx
Towards a code of practice for AI in AT.pptxJisc
 
REMIFENTANIL: An Ultra short acting opioid.pptx
REMIFENTANIL: An Ultra short acting opioid.pptxREMIFENTANIL: An Ultra short acting opioid.pptx
REMIFENTANIL: An Ultra short acting opioid.pptxDr. Ravikiran H M Gowda
 
FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024Elizabeth Walsh
 
Google Gemini An AI Revolution in Education.pptx
Google Gemini An AI Revolution in Education.pptxGoogle Gemini An AI Revolution in Education.pptx
Google Gemini An AI Revolution in Education.pptxDr. Sarita Anand
 
Interdisciplinary_Insights_Data_Collection_Methods.pptx
Interdisciplinary_Insights_Data_Collection_Methods.pptxInterdisciplinary_Insights_Data_Collection_Methods.pptx
Interdisciplinary_Insights_Data_Collection_Methods.pptxPooja Bhuva
 
The basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptxThe basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptxheathfieldcps1
 
ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.MaryamAhmad92
 
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptxBasic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptxDenish Jangid
 
On National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan FellowsOn National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan FellowsMebane Rash
 
Graduate Outcomes Presentation Slides - English
Graduate Outcomes Presentation Slides - EnglishGraduate Outcomes Presentation Slides - English
Graduate Outcomes Presentation Slides - Englishneillewis46
 

Último (20)

Micro-Scholarship, What it is, How can it help me.pdf
Micro-Scholarship, What it is, How can it help me.pdfMicro-Scholarship, What it is, How can it help me.pdf
Micro-Scholarship, What it is, How can it help me.pdf
 
Single or Multiple melodic lines structure
Single or Multiple melodic lines structureSingle or Multiple melodic lines structure
Single or Multiple melodic lines structure
 
Fostering Friendships - Enhancing Social Bonds in the Classroom
Fostering Friendships - Enhancing Social Bonds  in the ClassroomFostering Friendships - Enhancing Social Bonds  in the Classroom
Fostering Friendships - Enhancing Social Bonds in the Classroom
 
Understanding Accommodations and Modifications
Understanding  Accommodations and ModificationsUnderstanding  Accommodations and Modifications
Understanding Accommodations and Modifications
 
Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...
Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...
Jual Obat Aborsi Hongkong ( Asli No.1 ) 085657271886 Obat Penggugur Kandungan...
 
How to setup Pycharm environment for Odoo 17.pptx
How to setup Pycharm environment for Odoo 17.pptxHow to setup Pycharm environment for Odoo 17.pptx
How to setup Pycharm environment for Odoo 17.pptx
 
Plant propagation: Sexual and Asexual propapagation.pptx
Plant propagation: Sexual and Asexual propapagation.pptxPlant propagation: Sexual and Asexual propapagation.pptx
Plant propagation: Sexual and Asexual propapagation.pptx
 
SOC 101 Demonstration of Learning Presentation
SOC 101 Demonstration of Learning PresentationSOC 101 Demonstration of Learning Presentation
SOC 101 Demonstration of Learning Presentation
 
COMMUNICATING NEGATIVE NEWS - APPROACHES .pptx
COMMUNICATING NEGATIVE NEWS - APPROACHES .pptxCOMMUNICATING NEGATIVE NEWS - APPROACHES .pptx
COMMUNICATING NEGATIVE NEWS - APPROACHES .pptx
 
2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx
2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx
2024-NATIONAL-LEARNING-CAMP-AND-OTHER.pptx
 
Towards a code of practice for AI in AT.pptx
Towards a code of practice for AI in AT.pptxTowards a code of practice for AI in AT.pptx
Towards a code of practice for AI in AT.pptx
 
REMIFENTANIL: An Ultra short acting opioid.pptx
REMIFENTANIL: An Ultra short acting opioid.pptxREMIFENTANIL: An Ultra short acting opioid.pptx
REMIFENTANIL: An Ultra short acting opioid.pptx
 
FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024FSB Advising Checklist - Orientation 2024
FSB Advising Checklist - Orientation 2024
 
Google Gemini An AI Revolution in Education.pptx
Google Gemini An AI Revolution in Education.pptxGoogle Gemini An AI Revolution in Education.pptx
Google Gemini An AI Revolution in Education.pptx
 
Interdisciplinary_Insights_Data_Collection_Methods.pptx
Interdisciplinary_Insights_Data_Collection_Methods.pptxInterdisciplinary_Insights_Data_Collection_Methods.pptx
Interdisciplinary_Insights_Data_Collection_Methods.pptx
 
The basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptxThe basics of sentences session 3pptx.pptx
The basics of sentences session 3pptx.pptx
 
ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.ICT role in 21st century education and it's challenges.
ICT role in 21st century education and it's challenges.
 
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptxBasic Civil Engineering first year Notes- Chapter 4 Building.pptx
Basic Civil Engineering first year Notes- Chapter 4 Building.pptx
 
On National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan FellowsOn National Teacher Day, meet the 2024-25 Kenan Fellows
On National Teacher Day, meet the 2024-25 Kenan Fellows
 
Graduate Outcomes Presentation Slides - English
Graduate Outcomes Presentation Slides - EnglishGraduate Outcomes Presentation Slides - English
Graduate Outcomes Presentation Slides - English
 

Singapore grouper iridovirus induced parapoptosis-like death in host cells via the activation of MAPK signaling

  • 1. Singapore grouper iridovirus (SGIV) induced parapotosis-like cell death in host cells via the activation of MAPK signalings Qiwei Qin South China Sea Institute of Oceanology Chinese Academy of Sciences 2013.7.27
  • 2. programmed cell death , PCD PCD is natural and genetically regulated process of removing unneeded or damaged cells, and it plays critical roles in the balance between host defense and the invading pathogen during pathogen-host co-evolution. Research background (Dale E. Bredesen, 2007)
  • 3. Morphological and biochemical characteristics of apoptosis and parapoptosis (Sperandio et al., 2000)
  • 4. Virus infection induced PCD Virus infection induced apoptosis. Virus infection induced autophagic PCD So far, no report on virus infection induced parapoptosis !
  • 5. MAPK signaling pathways and PCD MAPKs have been identified: extracellular signal- regulated kinase (ERK), c-Jun N-terminal kinases (JNK) and p38 MAPK ( Wada et al, 2004 )。 Mitogen-activated protein kinase (MAPK) cascades are evolutionary conserved intracellular signaling pathways that regulate gene expression, mitosis, proliferation, metabolism and programmed cell death.
  • 6. MAPKs and virus infection MAPK (Han, Nat Immunol, 2006) TLR could recognize the PAMPs of pathogens (virus, bacterial) and trigger the host immune responses, including the activation of MAPK signaling pathways.
  • 7. Virus infection could activate MAPK pathways in mammalian and humans. Aquatic animal viruses activate MAPK signaling pathways ??
  • 8. Iridovirus is one of the most important infectious pathgens for maricultured grouper, Epinephelus spp ! Singapore grouper iridovirus (SGIV) isolated from diseased grouper is a novel species of ranavirus, family iridovidae ( Qin et al., 2003).
  • 9. 1. How the SGIV interact with grouper host cells causing cell death ? 2 、 What kind of host cell signaling pathways involve in the virus infection ?
  • 10. Huang et al, Aquaculture, 2009 SGIV infected grouper cells Lai et al, J Fish Dis. 2008 GIV infected barramundi cells Apoptosis Non-apoptosis Whether SGIV infection induced PCD is cell type dependent ?
  • 11. Research results 1.SGIV infection induced different type of cell death and nucleus morphology in host and non-host two fish cells Cell types Characteristics SGIV-EAGS rounded, congregated, remain attach, condensed nuclei, non-apoptotic bodies SGIV-FHM rounded, detached, apoptotic bodies observed
  • 12. Cells Characteristics SGIV-FHM DNA fragementation, DNA ladder, TUNEL positive SGIV-EAGS no fragementation,no DNA ladder, TUNEL negetive DNA fragmentation and DAN content analysis of SGIV-infected two fish cells
  • 13. SGIV-infectedEAGS: appearance of cytoplamic vaculoes, distended endoplasmic reticulum (ER), large cluster of swollen mitochondria. SGIV-FHM: ER swelling
  • 14. Examination of PS externalization and caspae activities The exposure of PS on cell surface is a general marker of apoptotic cells. The exposure of PS only occurred on the FHM cell surface during SGIV infection. Caspase-3 and -9 were activated in SGIV-infected FHM cells, but not in infected EAGS cells.
  • 15. UV-inactivated SGIV had not effect on EAGS cells, but still evoked obviously apoptosis in FHM cells. Virus replication was essential for SGIV induced nonapoptotic cell death, but not for apoptosis.
  • 16. SGIV infection activated MAPK signaling molecules in grouper cells ERK, p38 MAPK and JNK molecules were phosphorylated at the different stages during SGIV infection in host EAGS cells.
  • 17. Inhibition of ERK and JNK activities could delay the CPE and reduce the virus production. MAPK signaling pathways were involved in SGIV replication in grouper host cells
  • 18. Inhibition of JNK pathway could inhibit SGIV gene transcription and protein synthesis. No obvious changes for inhibition of p38 MAPK. JNK but not p38 MAPK is important for SGIV transcription and protein synthesis
  • 19. SGIV infection evoked increased the expression of IL-8, IRF-1 and TNFa, inhibition of JNK resulted in the reduction of 3 immune genes, inhibition of p38 MAPK only reduced the expression of Fish immune genes were regulated by MAPKs IL-8 IRF-1 TNF-a
  • 20. Conclusions SGIV infection induced nonapoptotic cell death-parapotosis in grouper host cells.
  • 21. MAPK signaling pathways involved in the SGIV infection and replication in host cells. ERK 、 JNK signals are essential for virus replication and virus induced parapotosis SGIV-activated MAPK pathways could modulate the expression of immune genes and inflammatory cytokines
  • 22. Related publications: 1. Huang X., Huang Y., Ouyang Z., Cai J., Qin Q. 2011. J Gen Virol., Jun; 92:1292-301. 2. Huang X., Huang Y., Ouyang Z., Xu L, Yan Y, Cui H, Han X, Qin Q. 2011. Apoptosis, Aug;16(8):831-45.
  • 23. 23 Acknowledges: National Basical Research Program(973 program); NSFC; CAS
  • 24. Thank you very muchThank you very much for your attentionfor your attention !!

Notas del editor

  1. 我们研究成果的主要创新点是 1 )。。。, 2 )。。。, 3 )。。。